Báo cáo y học: "Evidence-ranked motif identification" pdf

... cellular glycosylation of the enzyme efficiently prevents the electron from erroneously jumping out of the protein, whereas just the opposite is required for biosensor design. Deglycosylation ... evolution heavily recycles molecules. Proteomic analysis of the mammalian neuronal synapse revealed that 25% of the synaptic proteins are conserved in as simple an organism as yeast. In yeast,...

Ngày tải lên: 09/08/2014, 20:20

... with Cy3Label with Cy5 Label with Cy5Label with Cy3 Transcription factor of interest Other transcription factors DNA methylase Methylated DNA Hybridize to microarray Hybridize to microarray Immunoprecipitate with ... be used to verify targets by PCR or dot blot, or the DNA may be sub-cloned and sequenced to identify new targets [44]. For ChIP array analysis, the purified DNA is amplified by P...

Ngày tải lên: 09/08/2014, 20:21

... was always greater than the sum of the enhancements pro- duced by each individual motif. The degree of synergy was verified using the synergy index (see Materials and methods) described by Segre ... correspond- ing single motif insertion constructs. The synergy index for promoting splicing inclusion is defined as SI = W UD - W U ×W D . SI <0 indic ates synergy, SI = 0 in dicates no syne...

Ngày tải lên: 09/08/2014, 20:22

... experimentally using both the enzyme inhibition assay as w ell as the cell growth inhibition assay. It is indeed gratifying that two of t he six compounds clearly exhibit enzyme inhibition. This clearly ... measurements were made in triplicates. The genes GARS (glyc yl-tRNA synthetase), AGPAT1 (1-acylglycero l-3-phosphate O- acyltransferase 1), ATP5G1 [ATP synthase, H + trans- porting, mitoch...

Ngày tải lên: 10/08/2014, 09:22

... = 512). Given a motif m, we con- struct a candidate set of motifs by locally varying the length and the degeneracy of m.Theextensionmove takes a k-mer as input and independently appends or prepends ... datasets analyzed bycERMIT,cMyc,nMyc,E2f1,CTCF,Esrrb,Klf4, Nanog, Oct4, Sox2, STAT3, Tcfcp2I1 , and Zfx, were used as provided by [40]. The embryonic stem cell panel additionally included dat...

Ngày tải lên: 09/08/2014, 20:21

... [27]. Protein extraction and Western blot analysis Yeast protein extracts were obtained by direct lysis of yeast cells in 2 · SDS gel-loading buffer by a freeze/thawing cycle and boiling, according to Sambrook ... by lysis of the pelleted cells in 60 lL lysis buffer A(20m M Tris, 1 m M EDTA, 0.1% Nonidet P40, 25% glycerol, 20 m M Na-molybdate, pH 6.8), with addition of 0.3 M NaCl, followed by...

Ngày tải lên: 17/03/2014, 10:20

... haracteristics. They retain 37% and 23% of the wild-type A TPase activity, respectively, and are poorly phosphorylated by ATP and P i . Low but significant ATPase activity and phosphorylation by ATP were ... P709, respectively, are located near the phosphorylated D351 and constitute part of the phosphorylation site [4]. The crystal structure by Toyoshima et al.[4]showsthat D703 may be hydrogen...

Ngày tải lên: 17/03/2014, 17:20

... transhydro- genase assay described by Gan et al.[25]. Thioredoxin reductase activity assays Assays for thioredoxin reductase activity were carried out by two methods at 30 °C. In the NBS 2 reduction assay [26], ... identity and 73% similarity) from Sulfolobus solfataricus and shows a relat- ively high homology to the TR of E. coli (34% identity and 54% similarity). This protein possesses thre...

Ngày tải lên: 23/03/2014, 21:20

... models and high-density oligonucleotide arrays, in which approximately 36,000 genes and ESTs were analyzed. We analyzed whole synovial tissues instead of using specific cell types because we are ... Sugamura K, Hinuma Y: Human Retroviruses: HTLV-I and HTLV- II Volume 2. New York: Plenum Press; 1993. 7. Iwakura Y, Saijo S, Kioka Y, Nakayama-Yamada J, Itagaki K, Tosu M, Asano M, Kanai Y, K...

Ngày tải lên: 09/08/2014, 08:22

... Antibodies to N-homocysteinylated albumin as a marker for early-onset coronary artery disease in men. Thromb Haemost 2005, 93:346-350. 38. Yokoi T, Nagayama S, Kajiwara R, Kawaguchi Y, Horiuchi R, ... organization, bristle morphology, and viability are affected by actin capping protein mutations in Drosophila. J Cell Biol 1996, 133:1293-1305. 49. Hug C, Jay PY, Reddy I, McNally JG, Bridgman...

Ngày tải lên: 09/08/2014, 08:23