Báo cáo y học: "Gene ontology analysis for RNA-seq: accounting for selection bias" pdf

... reproduction in any medium, provided the original work is properly cited. Method Gene ontology analysis for RNA-seq: accounting for selection bias Matthew D Young, Matthew J Wakefield, Gordon K Smyth and ... platforms [5]. When perform- ing systems biology analyses, failure to account for this effect will lead to biased results. One simple, but extremely widely used, systems b...

Ngày tải lên: 09/08/2014, 20:21

... profiling. I will focus on the gene expression analysis of T lymphocytes, the key players in several inflammatory diseases, and on the microarray analysis of brain tissue from patients with multiple ... variability. To exert their functions, type 1 and type 2 T lymphocytes have to home into different sites. We reported an increased expression of mRNA for fucosyltransferase VII, which co...

Ngày tải lên: 09/08/2014, 01:21

... activated PBMC cDNA significantly improves assay reliability by reducing variation and by simplifying assay development. Analysis of RA synovia indicates that six or more synovial tissue fragments should ... Similarly, synovial fluid can be obtained rel- atively easily by joint aspiration but its utility is markedly diminished by the fact that the volumes are highly variable and effusions a...

Ngày tải lên: 09/08/2014, 01:23

... unpublished data). Symbolic discriminant analysis of microarray data Relationships between gene expression variables are very complex and patterns that have clinical significance may take many forms. It ... for clinically distinct disorders within the autoimmune category. Consistent with this hypothesis, we have found that clustering analyses can be used to separate patients with early RA...

Ngày tải lên: 09/08/2014, 01:23

... genetic synovectomy, and it is the latest development in the tradition of synovectomy, as performed surgically, chemically or by radiation therapy. In RA the fibroblast-like type B synoviocyte is ... the synovium can transform into an invasive tissue (pannus) that destroys cartilage and bone. Synovectomy of a joint, performed surgically, chemically or by radiation therapy, immediately stops ....

Ngày tải lên: 09/08/2014, 01:24

... by gene array analysis were validated by real-time RT- PCR TaqMan ® analysis using the ABI Prism 7900 Sequence Detector System ® (PE Applied Biosystems, Foster City, CA, USA), as previously ... cluster analysis. Validation of microarray data for a subset of genes was performed by real-time RT-PCR Taq- Man ® (Applied Biosystems, Foster City, CA, USA) analysis, which provides a highl...

Ngày tải lên: 09/08/2014, 06:22

... this system is highly inefficient for web site devel- opers. For example, every gene portal needs to implement at some level the basic functionality for searching for genes (for example, by symbol, ... 'KEGG' layout shows biological pathways relevant to any particular gene of interest [15]. Finally, the 'Exon Atlas' layout displays a plugin showing data for a pr...

Ngày tải lên: 09/08/2014, 20:21

... rates at synonymous and non-synon- ymous sites remote from the inactivating deletion pro- vides the information necessa ry for the calculation. Given this assumption, the following equality holds: Zhang ... Nishikimi M, Ozawa T, Yagi K: Isolation and sequence analysis of a complementary DNA encoding rat liver L-gulono-gamma-lactone oxidase, a key enzyme for L-ascorbic acid biosynthesis....

Ngày tải lên: 09/08/2014, 20:21

... 2-octapre- nyl-3-methyl-6-methoxy-1,4-benzoquinol; 2omph, 2-octaprenyl-6- methoxyphenol; 2oph, 2-octaprenylphenol; 3ophb, 3-octaprenyl-4- hydroxybenzoate; ahcys, s-adenosyl-l-homocysteine; amet, ... it is used only under some environments). 2ohph, 2-octaprenyl-6-hydroxyphenol; 2ombzl, 2-octaprenyl-6-methoxy-1,4-benzoquinol; 2omhmbl, 2-octaprenyl-3- methyl-5-hydroxy-6-methoxy-1,4-benzoquinol...

Ngày tải lên: 09/08/2014, 20:21

... Biology Solutions Using R and Bioconductor. Edited by: Gentleman R, Carey V, Dudoit S, R Irizarry WH. New York: Springer; 2005:397-420. 25. Smyth GK: Linear models and empirical Bayes methods for ... Smith C, Smyth G, Tierney L, Yang JYH, Zhang J: Bioconductor: Open software development for computational biology and bioinformatics. Genome Biol 2004, 5:R80. Anders and Huber Genome Biology...

Ngày tải lên: 09/08/2014, 22:23