Báo cáo y học: " Assembling genomes using short-read sequencing technology" pptx

... BAC library of 16,848 clones using Phrap [5], confi rmed the assembly by BAC end sequencing, and refi ned it by sequencing 63 fosmid clones.  e resulting assembly contains 125,325 ... technology for mammalian-sized genomes was demonstrated by Simpson et al. using ABySS [13]. Table 1. Assembly statistics for maize, horse, panda, blue-stain fungus (G. clavigera) and P. syringae geno...

Ngày tải lên: 09/08/2014, 20:21

... folic acid, was synthesized and characterized as previously described [9,10]. Briefly, folic acid was first reacted with 2,2'-(ethylen- edioxy) bis(ethylamine) using di-isopropylcarbodimide ... arthropathy that consumes substantial personal, social, and economic costs. The synovial mem- brane in patients with RA is characterized by hyperplasia, by increased vascularity, and by an infil...

Ngày tải lên: 09/08/2014, 06:22

... the species phylogeny best supported by pre- vious phylogenetic analyses [19-21]: gains are assigned directly from the topology of gene family trees whereas the most likely positions of gene ... even though phylogeny-based orthology/paralogy identifica- tion is widely accepted as the most valid approach [1-4]. Recently, however, the problem has been largely recog- nized and increasingly addre...

Ngày tải lên: 09/08/2014, 20:21

... "If you were offered this product by your physician, how likely would you be to accept it?" using ratings of a 1–3, b 4, and c 5–7 on a 7-point scale, where 1 is 'very likely', ... only 24% indicated they would resist this type of therapy if recommended by their physician. Our results suggest that patients' perceptions of SIAT may be more positive than previousl...

Ngày tải lên: 10/08/2014, 05:21

... on early morbidity and mortality. J Card Surg 2006, 21:22-27. 2. Youn YN, Chang BC, Hong YS, Kwak YL, Yoo KL: Early and mid-term impacts of cardiopulmonary bypass on coronary artery bypass grafting Darwazah ... off- pump bypass technique. Methods: This retrospective study includes 137 patients with ejection fraction below 0.35 who underwent isolated coronary artery bypass surgery. 39 patients...

Ngày tải lên: 10/08/2014, 09:22

... variety of materials, are rela- tively inexpensive, and suitable for practical daily use due to their elasticity. In a pilot study involving 15 patients, they were found to be not significantly inferior to ... been systematically investigated in only a few studies, but have shown pro- mis ing results [4]. Relatively rigid ‘corset-like’ const ruc- tions can, as we have occasionally observed, e...

Ngày tải lên: 11/08/2014, 03:21

... efficacy of surgical interventions for secondary sternal stabilization, but individual pat ients may reject this option or may be, for other reasons, no longer operable. The task of primary care physicians ... D-06112 Halle, Germany. 2 Department of Cardiac and Thoracic Surgery, University Hospital Halle, Ernst Grube Str. 40, D-06120 Halle, Germany. Authors’ contributions MH analyzed and inte...

Ngày tải lên: 11/08/2014, 07:20

... manuscript. Author Details 1 Gastroenterology Division, Yokohama City University School of Medicine, Japan and 2 Department of Pathology, Yokohama City University Hospital, Japan References 1. Iishi ... report Tomoyuki Akiyama 1 , Yasunobu Abe 1 , Hiroshi Iida 1 , Hiroki Endo 1 , Kunihiro Hosono 1 , Kyoko Yoneda 1 , Hirokazu Takahashi 1 , Masahiko Inamori* 1 , Akihide Ryo 2 , Shoji Yamana...

Ngày tải lên: 11/08/2014, 12:20

... spectroscopy. Biochemistry 20, 952–958. 26. Schelvis, J.P.M., Kim, S .Y. , Zhao, Y. D., Marletta, M.A. & Babcock, G.T. (1999) Structural dynamics in the guanylate cyclase heme pocket after CO photolysis. ... stability of imidazole and 4-methyl imidazole ligands over the 1-methyl and 2-methyl imidazoles is likely due to the fact that the first two species are closest in binding geometry to...

Ngày tải lên: 08/03/2014, 16:20

... with the activity of bacterial RuvB, the INO80 complex contains DNA helicase activity and, moreover, ino80 null mutants display sensitivity to hydroxyurea, the alkylating agent methylmethane sulfonate, and ... cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6–4) pyrimidone photoproducts (6–4PPs), which induce a DNA bend or kink of 7–9° and 44°, respectively [11,12]. Correspondence to J....

Ngày tải lên: 31/03/2014, 21:21