Báo cáo y học: " Ubiquitous RNA-dependent RNA polymerase and gene silencing" pptx

... silencing by small RNAs other than as a defense against viruses and transposons. Elp1 is a novel RNA- dependent RNA polymerase Lipardi and Paterson [10] sought to purify the previously reported ... specificity of Elp1 for the RNAi pathway. Minireview Ubiquitous RNA- dependent RNA polymerase and gene silencing James A Birchler Address: Division of Biological Science...

Ngày tải lên: 09/08/2014, 20:20

... OA and RA synovial fluids and joint tissues, and is produced in vitro by both the synovium and chondrocytes in response to inflammatory cytokines [36]. MMP-14, produced principally by RA synovial ... since they do not divide and the matrix isolates them from each other, but gene expression and protein synthesis may be activated by injury. Since the ECM normally shields chondrocy...

Ngày tải lên: 09/08/2014, 01:22

... real-time PCR and Western blot analysis. Cells proliferation was evaluated by using flow cytometric anlysis and MTT assay. The abilities of invasion and migration were evaluated by cell migration and invasion ... cancer. Gene therapies for tumor were focused on in recent years, including gene replacement, antisense nucleic acid technique, cytokine gene therapy and RNA interf...

Ngày tải lên: 10/08/2014, 10:21

... methylation and microRNA (miRNA), can also lead to silencing of the target genes [32,33]. In fact, recently reported results suggest that DNA methylation may play an important role in XPC gene ... n/ deacetylation, and microRNA (miRNA). In regards to histone acetylation/deacetylation, it is widely known that the acetylation status of histones significantly affects transcription of targe...

Ngày tải lên: 10/08/2014, 21:23

... spliced and u nspliced genes (2,218 and 2,598 genes, respectively). Lists of spliced/un spliced genes were then ranked in descending order and split into 10 equal groups (approximately 220 and 260 genes ... microarray; FAIRE: formaldehyde-assisted isolation of regulatory elements; H3K36Me3: lysine 36 trimethylation of histone H3; ORF: open reading frame; PCR: polymerase chain reacti...

Ngày tải lên: 09/08/2014, 23:20

... PARP-1 gene, we employed post- transcriptional gene silencing technology by RNA inter- ference. Specifically, we utilized small interfering RNA (siRNA) t o silence PARP-1 gene and hypothesized that the ... direct interaction with HSF-1 and poly (ADP-ribosylation). Introduction Poly (ADP-ribose) polymerase- 1 (PARP-1) is a highly conserved chromatin bound enzyme [1,2] and plays...

Ngày tải lên: 11/08/2014, 03:20

... modulates transcription by direct interaction with AP2 [38], Oct-1 [39], YY-1 [40] and TEF-1 [41]. PAR P has been also shown to alter RNA polymerase II depen dent transcrip- tion [42] and to effectively prevent and ... in PARP-1deficientmiceisnotacompensatoryresponse to the missing PARP-1 gene, we employed post- transcriptional gene silencing technology by RNA inter- ference. S...

Ngày tải lên: 11/08/2014, 06:22

... mitogen-activated protein kinase and c-Jun NH 2 -terminal kinase by double-stranded RNA and en- cephalomyocarditis virus: involvement of RNase L, protein kinase R., and alternative pathways. Mol. Cell. Biol. ... °C. The phosphorylation reaction was resolved by SDS/ PAGE and visualized by autoradiography. PKR activity was measured as described [11]. Immunoprecipitation and Immunobl...

Ngày tải lên: 08/03/2014, 09:20

... LNCRNA: long noncoding RNA; MIRNA: microRNA; NCRNA: noncoding RNA; ORF: open reading frame; P: postnatal day; PRI-MIRNA: primary microRNA; RACE: rapid amplification of cDNA ends; RMST: rhabdomyosarcoma ... PCR4-TOPO vector (Invitrogen) and then sequenced to confirm authenticity. Sense and antisense probes were generated from selected PCR4-TOPO clones using T7 and T3 RNA polymerases...

Ngày tải lên: 09/08/2014, 20:22

... the counts and the outcome, by setting K =1ands k (·) to be the identify function; or (2) a factor model by setting K = # of groups and s k (·) =1 (y j = k). Myrna allows the user to specify either ... gene expression in large RNA- Seq datasets. We apply Myrna to the analysis of publicly available data sets and assess the goodness of fit of standard statistical models. Myrna is avai...

Ngày tải lên: 09/08/2014, 20:22