Báo cáo y học: "Advice and dissent" doc

... constantly told, acquire and retain power by deceit and salesmanship, and frequently are contemptuous of the people they profess to serve. But, true as that cliché might be (and happily there ... Martin Rees, the President of the Royal Society, sent the government a set of guidelines that they say “would enhance confidence in the scientific advisory system and help government...

Ngày tải lên: 09/08/2014, 20:20

... hipermodified base Y3 7 (wybutine) (Figs 6 and 7). Also other amino- glycosides, as kanamycin A, neomycin B and a synthetic neomycin-neomycin dimer have been shown to induce fragmentation of tRNA Phe at Y3 7 and ... 617– 618. 34. Yanagawa, H., Ogawa, Y. & Ueno, M. (1992) Redox ribonu- cleosides. Isolation and characterization of 5-ydroxyuridine, 8-hydroxyguanosine & 8-hydrox...

Ngày tải lên: 21/02/2014, 01:21

... O76865 and O9W4W6) and i ndicated by the arrowheads. The s ymbol ÔVÕ indicates a po ten tial N - glycosylation site. A putative polyadenylation signal is enclosed by a box. (B) Hydrophobicity plot ... m M phenylmethanesulfonyl¯uoride], and the samples were fractionated b y electrophoresis on a 12% SDS/polyacrylamide gel. The separated polypeptides were electotransferred to a poly(viny...

Ngày tải lên: 21/02/2014, 15:20

... structural and enzyme kinetic studies. Mutation of Tyr183 t o phenylalanine produced an enzym e with a lower k cat and a slightly higher K m than the wild-type enzyme. Mutating Asp125 and Glu127 ... (1995) Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and X-ray structure of a complex with allosamidin: evidence for substrate assisted catalysis. Biochemistry 34, 156...

Ngày tải lên: 22/02/2014, 04:20

... two-subunit enzyme (Fig. 2) and all mutants in positions 121 and 122 (W121F, W12 1Y, W121Q /Y1 22Q, W121F /Y1 22F, W121G and W12 1Y/ Y122W) were decreased to 36 m M .Ofthe remaining mutants, two showed wild-type ... analyzed by SDS/PAGE to verify their subunit composition, con- centrated and stored at )80 °C. Steady-state kinetics and determination of the ionic strength dependence C...

Ngày tải lên: 22/02/2014, 07:20

... Saccharomyces cerevisiae which is Ca 2+ -dependent, preferentially hydrolyses phosphatidyl- ethanolamine (PtdEtn) and phosphatidylserine and does not catalyse a transphosphatidylation with primary short-chain alcohols. ... catalytic proper- ties. Its activity is Ca 2+ -dependent; it preferentially hydrolyses phosphatidylethanolamine (PtdEtn) and phos- phatidylserine (PtdSer); and its...

Ngày tải lên: 22/02/2014, 07:20

... to homogeneity by HPLC and digested with t rypsin. The tryptic peptides were separated by HPLC and some of them were totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ... 2Cys-Prx enzymes previously described i n yeast, mammals and plants, Ch-Prx1 d isplayed antioxidant and peroxidase activities. The enzyme could reduce hydro- gen peroxide as we...

Ngày tải lên: 08/03/2014, 16:20

... Sequence analysis and molecular modelling predict the two EF-hand motifs characteristic of the S100 family. Metal-binding properties were studied by a direct 45 Ca 2+ -binding assay and by fluorescence ... SDS/PAGE analysis, only lungfish liver and intestine showed the calhepatin band (data not shown). Fig. 3. Primary structure of calhepatin and its sequence alignment with S100 family m...

Ngày tải lên: 08/03/2014, 22:20

... carboxylase activity. The effect of proFIX18, proPT18 and pro-e-TxIX/12 on FLEEL carboxylation by the recombinant bovine carboxylase (closed symbols) and the recom- binant Conus carboxylase (open symbols) ... on Conus carboxylase activity Our work [43] and that of Bandyopadhyay et al.[42] demonstrated the importance of the propeptide in directing c-carboxylation of Conus precursor substr...

Ngày tải lên: 17/03/2014, 10:20

... & Wolynes, P.G. (1995) Funnels, pathways and the energy landscape of protein folding: a synthesis. Proteins 21, 167–195. 2. Dill, K.A. & Chan, H.S. (1997) From Levinthal to pathways to funnels. ... Schimmel, P.R. (1980) Biophysical Chemistry. Part II: Techniques for the Study of Biological Structure and Function: Spectroscopic Analysis of Biopolymers. W. H. Freeman, New York, USA....

Ngày tải lên: 17/03/2014, 23:20