... (16,17,18). The presence of C. pneumoniae in the blood cells predetermines the possibility of pathogen dis-semination from respiratory system to different or-gans and tissues. Besides respiratory organs ... using the TA clon-ing kit (Invitrogen, San Diego, CA) similarly to Broc-colo F (25). The cycle threshold (CT) values, defined as the number of cycles at which the fluorescence of the ... MgCl2, 200 µM of each dNTPs, 1 U Taq-DNA-polymerase, 15 pmol of each primer. For-ty-five cycles of amplification were performed on a PCR Thermocycler Perkin Elmer. Each cycle consisted of denaturation...