Báo cáo y học: "High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans " potx

... Biology 2009, 10:R101 Open Access 2009Ramaniet al.Volume 10, Issue 9, Article R101 Research High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans Arun ... are defective for NMD using a combination of tiling arrays and sequencing (exactly as was done for the wild-type transcriptome above), and compare it to...

Ngày tải lên: 09/08/2014, 20:20

... implants (brachytherapy) [7]. iv) The standard initial systemic therapy for locally advanced or metastatic disease is hormonal or androgen depri- vation therapy (ADT) that may be performed by bi- lateral ... Constitutional formula of the investigated TMZ-BioShuttle Cell Cycle and Cell Death Analysis The effects on the cell viability and the cell cycle distribution were determined...

Ngày tải lên: 26/10/2012, 09:48

... corre- sponding histology (b). Areas of hypocellularity are indicated by the red arrows. Figure 4 An optical coherence tomography (OCT) image (a) and the corre-sponding histology (b) of severely degenerated ... banding pattern is dis- rupted and correlates with histologically abnormal staining and cellularity. Fig. 4 shows an OCT image (Fig. 4a) and the correspond- ing histology (Fig...

Ngày tải lên: 09/08/2014, 06:22

... -0.70 and 0.45 were chosen for homozygous deletions and multi-copy gain events, respectively. For each individual Yoruba sample, sets of probes for each CNV were analyzed separately by CBS, and ... CNVs, and type large numbers of individuals in order to gain biological insights beyond the initial discovery. Materials and methods Array synthesis Arrays were synthesized followin...

Ngày tải lên: 09/08/2014, 20:20

... thank Sandra Clauder-Muenster for technical assistance, Vladimir Benes and Tomi Baehr-Ivacevic from EMBL GeneCore Facility for technical advi ce, Yury Belyaev and Arne Seitz from EMBL-ALMF for help ... DNA-free Kit, Foster City, CA, USA) for 25 minutes at 37°C according to the manufacturer’ s instructions and subsequently reverse transcribed to single-stranded cDNA for microar...

Ngày tải lên: 09/08/2014, 20:21

... cofactor for L. donovani interaction with ECM and BM. Both Zn 2+ and free sulfhydryls may be required for LBP binding site on laminin a s e videnced by the stimulatory and inhibitory effects ... [ 35 S]methionine, lysed and the LBP w as immunoprecipi- tated by anti-LBP Ig and autoradiographed (lane 6). 1624 K. Bandyopadhyay et al. (Eur. J. Biochem. 269) Ó FEBS 2002 (Fig. 2 B). B...

Ngày tải lên: 08/03/2014, 23:20

... Lyrodus pedicellatus, were incubated in seawater in a 15 N atmosphere, fixed in formaldehyde, embedded in epon, and sectioned for MIMS analysis. Adipocytes Adipocytes were prepared and analyzed ... glutaraldehyde) for 3 days, rinsed in deion- ized water, dehydrated in ethanol and propylene oxide, infused with propylene oxide/epon, and embedded in epon. Other tissues were treated...

Ngày tải lên: 06/08/2014, 18:21

... Botany, Gregor Mendelstrasse 33, 1180 Vienna, Austria b CSIRO Forestry and Forest Products, GPO Box 252-12, Hobart, TAS 7001, Australia c CSIRO Forestry and Forest Products, Private Bag 10, Clayton ... July 2001; accepted 11 February 2002) Abstract – Wood density is the most important determinant of wood quality and a critical factor in short rotation forestry. Daily radial growth of si...

Ngày tải lên: 08/08/2014, 14:20

... 94°C for 5 min, followed by 35 cycles at 94°C for 1 min, 62-67°C for 1 min and 72°C for 1 min, followed by a final incubation at 72°C for 5 min. Screening of the TILLING library Amplicons analyzed ... kernels was deter- mined by Megazymes Total Starch Assay Kit (AA/ AMG, Megazyme Pty Ltd., Wicklow, Ireland). Acknowledgements This study is partially financially supported by the Eur...

Ngày tải lên: 11/08/2014, 11:21

... unpublished results). There is one dystrophin- and one dystrobrevin-like gene in thegenomeofC. elegans (dys-1 and dyb-1, respectively) [16,17]. C. elegans dystrophin and dystrobrevin are able t o bind ... 2¢5), greatly reduce t he interactions between Dys-1 and D yb-1 in the yeast system. Functional complementation of Dyb-1 deletions in C. elegans The only functional assay avail...

Ngày tải lên: 08/03/2014, 23:20