Báo cáo y học: "High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans " potx
Báo cáo y học: "High resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans." potx
... Biology 2009, 10:R101Open Access2009Ramaniet al.Volume 10, Issue 9, Article R101ResearchHigh resolution transcriptome maps for wild-type and nonsense-mediated decay-defective Caenorhabditis elegans Arun ... are defective for NMD using a combination of tilingarrays and sequencing (exactly as was done for the wild-type transcriptome above), and compare it to our wild-type refer-ence transcriptome ... pathways [8,9]. Recently, genome-scale tiling arrays and massively parallel sequence analysis of transcriptomes haveemerged as powerful new tools for transcriptome analysis[10-14]. Both rely on...
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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... implants (brachytherapy) [7]. iv) The standard initial systemic therapy for locally advanced or metastatic disease is hormonal or androgen depri-vation therapy (ADT) that may be performed by bi-lateral ... Constitutional formula of the investigated TMZ-BioShuttle Cell Cycle and Cell Death Analysis The effects on the cell viability and the cell cycle distribution were determined by DNA flow cytome-try. ... diploid lymphocytes was 0.8 - 1.0. Cell viability and apoptotic cells were assessed by flow cytometry with propidium iodide (PI)-method. For detection of apoptotic cells and vi-ability a FACS...
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Báo cáo y học: "High-resolution optical coherence tomographic imaging of osteoarthritic cartilage during open knee surgery" ppsx
... corre-sponding histology (b). Areas of hypocellularity are indicated by the red arrows.Figure 4An optical coherence tomography (OCT) image (a) and the corre-sponding histology (b) of severely degenerated ... banding pattern is dis-rupted and correlates with histologically abnormal staining and cellularity.Fig. 4 shows an OCT image (Fig. 4a) and the correspond-ing histology (Fig. 4b) of severely ... tape for future analysis.The protocol for OCT imaging during open knee surgerywas approved by the investigational review board of theMassachusetts Institute of Technology and West RoxburyVeterans...
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Báo cáo y học: "High resolution discovery and confirmation of copy number variants in 90 Yoruba Nigerians" pdf
... -0.70 and 0.45 were chosen for homozygousdeletions and multi-copy gain events, respectively. For eachindividual Yoruba sample, sets of probes for each CNV wereanalyzed separately by CBS, and ... CNVs, and type large numbers of individuals inorder to gain biological insights beyond the initial discovery.Materials and methodsArray synthesisArrays were synthesized following standard Affymetrix ... withearlier low resolution bacterial artificial chromosome (BAC)arrays followed by commercially available array comparativegenome hybridization (CGH) and SNP genotyping arrays,have steadily driven...
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Báo cáo y học: " High-resolution transcription atlas of the mitotic cell cycle in budding yeast" pptx
... thank Sandra Clauder-Muenster for technical assistance, Vladimir Benes and Tomi Baehr-Ivacevic from EMBL GeneCore Facility for technical advi ce,Yury Belyaev and Arne Seitz from EMBL-ALMF for help ... DNA-free Kit, Foster City, CA, USA) for 25minutes at 37°C according to the manufacturer’ sinstructions and subsequently reverse transcribed tosingle-stranded cDNA for microarray hybridization.Each ... periodi-city. Between 400 and 800 periodically expressed* Correspondence: larsms@embl.de† Contributed equally1EMBL - European Molecular Biology Laboratory, Department of GenomeBiology, Meyerhofstr....
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Báo cáo Y học: High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences doc
... cofactor for L. donovani interaction with ECM and BM. Both Zn2+ and free sulfhydryls may be required for LBP binding site onlaminin a s e videnced by the stimulatory and inhibitoryeffects ... [35S]methionine, lysed and the LBP w as immunoprecipi-tated by anti-LBP Ig and autoradiographed (lane 6).1624 K. Bandyopadhyay et al. (Eur. J. Biochem. 269) Ó FEBS 2002(Fig. 2 B). Both affinity and binding ... sequencesKeya Bandyopadhyay, Sudipan Karmakar, Abhijit Ghosh and Pijush K. DasMolecular Cell Biology Laboratory, Indian Institute of Chemical Biology, Jadavpur, Calcutta, IndiaIn the course of trying...
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Báo cáo sinh học: "High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry" docx
... Lyrodus pedicellatus, were incubated in seawaterin a 15N atmosphere, fixed in formaldehyde, embedded inepon, and sectioned for MIMS analysis.Adipocytes Adipocytes were prepared and analyzed ... glutaraldehyde) for 3 days, rinsed in deion-ized water, dehydrated in ethanol and propylene oxide,infused with propylene oxide/epon, and embedded in epon.Other tissues were treated similarly, but ... usingformaldehyde and glutaraldehyde; detached from plasticwith propylene oxide; embedded in epon-Araldite; sec-tioned at 0.5 m thickness; and mounted on silicon chips for analysis.Tuning for...
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Báo cáo khao học: "High-resolution analysis of radial growth and wood density in Eucalyptus nitens, grown under different irrigation regimes" potx
... Botany, Gregor Mendelstrasse 33, 1180 Vienna, AustriabCSIRO Forestry and Forest Products, GPO Box 252-12, Hobart, TAS 7001, AustraliacCSIRO Forestry and Forest Products, Private Bag 10, Clayton ... July 2001; accepted 11 February 2002)Abstract – Wood density is the most important determinant of wood quality and a critical factor in short rotation forestry. Daily radial growth ofsix-year-old ... of lower den-sity formed during the early growing season and higher den-sity formed during the later part [4]. Particularly theirrigated-droughted trees, with expressed cycles of soil-waterdeficits...
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báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes" doc
... 94°C for 5 min, followed by 35 cycles at 94°C for 1min, 62-67°C for 1 min and 72°C for 1 min, followed bya final incubation at 72°C for 5 min.Screening of the TILLING libraryAmplicons analyzed ... kernels was deter-mined by Megazymes Total Starch Assay Kit (AA/AMG, Megazyme Pty Ltd., Wicklow, Ireland).AcknowledgementsThis study is partially financially supported by the European Commission ... M, Yamamoto Y, Satoh H, Shimamoto K: Nonsense-mediated decayof mutant waxy mRNA in rice. Plant Physiol 2001, 125:1388-1395.50. Miura H, Sugawara A: Dosage effect of Waxy genes on amylose synthesisin...
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Báo cáo Y học: Dystrobrevin requires a dystrophin-binding domain to function in Caenorhabditis elegans doc
... unpublished results).There is one dystrophin- and one dystrobrevin-like gene inthegenomeofC. elegans (dys-1 and dyb-1, respectively)[16,17]. C. elegans dystrophin and dystrobrevin are able t obind ... 2¢5),greatly reduce t he interactions between Dys-1 and D yb-1 inthe yeast system.Functional complementation of Dyb-1 deletionsinC. elegans The only functional assay available for dystrobrevin ... the same way as theirmammalian counterparts [12], and they also bind tosyntrophin [18]. dys-1 and dyb-1 mutants d isplay a similarbehavioural phenotype c onsisting of hyperactivity, exagger-ated...
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