Báo cáo sinh học: "Assignment of PCR markers to river buffalo chromosomes" pptx
Báo cáo sinh học: "Assignment of PCR markers to river buffalo chromosomes" pptx
... syntenic relationship of ten markers in buffalo, and to map them to buffalo chromosomes on the basis of thecomparative banding homoeology between buffalo and cattle.2. ... H.A., Othman O.E., Bosma A., de Haan N., Assignment of genes to chromosome 4 of the river buffalo with a panel of buffalo- hamster hybridcells, J. Anim. Breed. Genet. ... homoeology be-tween cattle and river buffalo chromosomes [22]. Genes assigned to specific cattlechromosomes have been assigned to homoeologous buffalo chromosomes [6, 7, 10,11,...
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Báo cáo sinh học: " Assignment of CPS1, OTC, CRYD2, ARG2 and ASS genes to the chicken RH map" pot
... used to assign the five genes via a mapping tool which is a clone of the porcine IMpRH server [10]. Distances and log of odds (lod) scores werecalculated according to Lange et al. [7] relative to ... genomicDNA using the primer-pair was confirmed to be identical to that of cDNA.Assignment of five genes to chicken RH map 599[2] Campbell J.W., Excretory nitrogen metabolism, in: Prosser C.L. (Ed.),Environmental ... Enzymes of arginine metabolism in chicks, Arch. Biochem.Biophys. 102 (1963) 249–258.598 T. Shimogiri et al.CRYD2 was calculated to link to CRK with the highest lod score of 14.16and to HSA277841...
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Báo cáo sinh học: " Usefulness of Herpes Consensus PCR methodology to routine diagnostic testing for herpesviruses infections in clinical specimens" docx
... recognition isimportant to ensure adequate antiviral treatment whenavailable. The ability of Consensus PCR to yield resultsfrom ocular specimens has been of particular interest and of considerable ... mayalso be useful for monitoring the efficacy of treatment[20].According to our experience, Consensus PCR assay can beuseful to facilitate the routine diagnosis of herpesvirusesinfections, ... withthe Consensus PCR assay. To our knowledge, this is the first time that the experience of a single clinical microbiology laboratory has beenreported since the commercial availability of theimproved...
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Báo cáo sinh học: " Discovery of herpesviruses in multi-infected primates using locked nucleic acids (LNA) and a bigenic PCR approach" pdf
... sec-ond round of pan-herpes DPOL PCR was performed asreal-time PCR. The PCR mix was made up to a volume of 25 μl containing 1.5 μl of the first round reaction product,1 × PCR buffer, 2 ... respectively.Pan-herpes DPOL PCR of chimpanzee samples in the pres-ence or absence of LNAFigure 4Pan-herpes DPOL PCR of chimpanzee samples in the presence or absence of LNA. Pan-herpes DPOL PCR was carried ... 90°C), and the number of cycles was increased from 45 to 50.Long-distance PCR LD -PCR was performed with the TaKaRa-Ex PCR system(Takara Bio Inc., Japan) or the Long-template PCR system(Roche,...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... 106 pergram of stool to 7.53 × 109 molecules per gram of stool.These high levels are consistent with numbers reported inother studies of NoV levels in stools [7,10].The basis of this quantification ... step of 84°C for 10 s to melt primer dimers.For the creation of standard curves, 2 µl containing dilu-tions of 107 to 101 molecules of GI/2 plasmid or 5 × 107 to 5 × 101 molecules of ... wereperpendicular to the previous position. 15 µl of each PCR product in 135 µl of 2 × SSPE-0.1% SDS was denatured byheating to 99°C for 10 min and chilled on ice. The slotswere then filled with 150 µl of PCR...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... shortlist of potential housekeeping genes to use as normalisers for QPCR experiments.BackgroundQuantitative real-time PCR (QPCR) is an invaluable toolfor the measurement of target nucleic acid sequences ... integrity, the efficiency of the reverse tran-scription of RNA to cDNA and the amount of sampleloaded. References for normalising sample and run varia-tion include the use of total nucleic acid concentrations,rRNA ... of viral infections. Nevertheless it is of benefit for researchers to be able to determine a shortlist of potential candidates. To date the variability of housekeep-ing gene expression has been...
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Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes" pptx
... strategy to determine the complete sequence of a region of the divergent locus B of a macaque homolog of KSHVFigure 12CODEHOP strategy to determine the complete sequence of a region of the divergent ... characteristic of gammaherpesviruses, is shown. The position of the CODEHOP PCR primers used to obtain the sequence of the entire DNA polymerase gene of RFHVMn and RFHVMm is shown. The overlapping PCR ... ER, Raden BW, Tsai CC: Analysis of 4.3Kb of the divergent locus-B of macaque retroperitonealfibromatosis-associated herpesvirus (RFHV) reveals closesimiliarity to Kaposi's sarcoma-associated...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... expressed as copies of viral DNAper 100 µL of virus suspension. The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... used for PCR in a final volume of 20 µl of PCR mixture. Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension. ... batches of JCV(Mad1) (I and II) were serially dilutedtwo-fold (64 HA to 1 HA) and then 10-fold (1 HA to 0.001 HA) in DMEM, to obtain HA titers ranging from 64HAU to 0.001 HAU per 100 µL of the...
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Báo cáo khoa học: " Identification of PCR-based markers linked to wood splitting in Eucalyptus grandis" pdf
... of de-tection is drastically reduced by targeting only very closely linked markers. High numbers of potential markers need to be analysed. If,however, markers are found, these are likely to ... bands were scored on a scale rangingfrom 0 to 4 according to the intensity of the bands. A score of 0 rep-resented the absence of a band and a score of 4 a very dark band.In addition the bulked ... unlinked markers and markers in linkage equilibrium with QTL are expected to be randomly dis-tributed across bulks [1]. Only markers that are very tightly linked to the QTL are likely to show...
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Báo cáo sinh học: " Evolution of the polymorphism at molecular markers in QTL and non-QTL regions in selected chicken lines (Open Access publication)" ppsx
... thesupposedly neutral markers (c). Black circles refer to G2, black squares to G1 andwhite items refer to G11: circles refer to line 1, squares to line 2, triangles to line 3 anddiamonds to the control ... all markers. Second, in order to assess the influence of the differenttypes of markers, PCAwas based, on genotypes at the supposedly neutral markers, on the one hand and on genotypes at markers ... assumption of pure d rift: transmission of haplotypes a ndgenotypes followed Mendelian transmission rules. Second, we assumed thepresence of QTL related to one of the three traits in one of the...
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