Báo cáo sinh học: "Assignment of PCR markers to river buffalo chromosomes" pptx

... syntenic relationship of ten markers in buffalo, and to map them to buffalo chromosomes on the basis of the comparative banding homoeology between buffalo and cattle. 2. ... H.A., Othman O.E., Bosma A., de Haan N., Assignment of genes to chromosome 4 of the river buffalo with a panel of buffalo- hamster hybrid cells, J. Anim. Br...

Ngày tải lên: 09/08/2014, 18:22

... used to assign the five genes via a mapping tool which is a clone of the porcine IMpRH server [10]. Distances and log of odds (lod) scores were calculated according to Lange et al. [7] relative to ... genomic DNA using the primer-pair was confirmed to be identical to that of cDNA. Assignment of five genes to chicken RH map 599 [2] Campbell J.W., Excretory nitrogen metabolism,...

Ngày tải lên: 14/08/2014, 13:22

... recognition is important to ensure adequate antiviral treatment when available. The ability of Consensus PCR to yield results from ocular specimens has been of particular interest and of considerable ... may also be useful for monitoring the efficacy of treatment [20]. According to our experience, Consensus PCR assay can be useful to facilitate the routine diagnosis of h...

Ngày tải lên: 18/06/2014, 18:20

... sec- ond round of pan-herpes DPOL PCR was performed as real-time PCR. The PCR mix was made up to a volume of 25 μ l containing 1.5 μ l of the first round reaction product, 1 × PCR buffer, 2 ... respectively. Pan-herpes DPOL PCR of chimpanzee samples in the pres-ence or absence of LNAFigure 4 Pan-herpes DPOL PCR of chimpanzee samples in the presence or absence of...

Ngày tải lên: 18/06/2014, 18:20

... 10 6 per gram of stool to 7.53 × 10 9 molecules per gram of stool. These high levels are consistent with numbers reported in other studies of NoV levels in stools [7,10]. The basis of this quantification ... step of 84°C for 10 s to melt primer dimers. For the creation of standard curves, 2 µl containing dilu- tions of 10 7 to 10 1 molecules of GI/2 plasmid or 5 × 10...

Ngày tải lên: 18/06/2014, 18:20

... shortlist of potential housekeeping genes to use as normalisers for QPCR experiments. Background Quantitative real-time PCR (QPCR) is an invaluable tool for the measurement of target nucleic acid sequences ... integrity, the efficiency of the reverse tran- scription of RNA to cDNA and the amount of sample loaded. References for normalising sample and run varia- tion include th...

Ngày tải lên: 18/06/2014, 18:20

... strategy to determine the complete sequence of a region of the divergent locus B of a macaque homolog of KSHVFigure 12 CODEHOP strategy to determine the complete sequence of a region of the divergent ... characteristic of gammaherpesviruses, is shown. The position of the CODEHOP PCR primers used to obtain the sequence of the entire DNA polymerase gene of RFHVMn...

Ngày tải lên: 18/06/2014, 22:20

... expressed as copies of viral DNA per 100 µL of virus suspension. The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... used for PCR in a final volume of 20 µl of PCR mixture. Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of vira...

Ngày tải lên: 19/06/2014, 08:20

... of de - tection is drastically reduced by targeting only very closely linked markers. High numbers of potential markers need to be analysed. If, however, markers are found, these are likely to ... bands were scored on a scale ranging from 0 to 4 according to the intensity of the bands. A score of 0 rep - resented the absence of a band and a score of 4 a very dark band...

Ngày tải lên: 08/08/2014, 14:20

... the supposedly neutral markers (c). Black circles refer to G2, black squares to G1 and white items refer to G11: circles refer to line 1, squares to line 2, triangles to line 3 and diamonds to the control ... all markers. Second, in order to assess the influence of the different types of markers, PCAwas based, on genotypes at the supposedly neutral markers, on the one...

Ngày tải lên: 14/08/2014, 13:21