... 10 )5 m), 202 (5. 2 · 10 )5 m), 304(3.4 · 10 )5 m), 307 (3.7 · 10 )5 m), 403 (5. 0 · 10 )5 m),404 (5. 4 · 10 )5 m), and 4 05 (5. 3 · 10 )5 m). Theiraffinities for CCA were similar (3 5 · 10 )5 m), ... occurs and the concentration of oligosaccharides in the elution finally reaches a plateau,where the concentration is equal to that of the initial solu-tion (Fig. 6B). In the case of oligosaccharides ... determination of Kd, retarda-tion of the elution front relative to that of PA-lactose,i.e. V–V0, was measured for each analyte solution dilu-ted to either 2 .5 or 5. 0 nm. The amount of glycanrequired...