Báo cáo khoa hoc:"Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels" pps

... 10.1051/gse:2001006 Original article Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels Fiona C. B UCHANAN a, ∗ , Carolyn J. F ITZSIMMONS b , Andrew G. V AN K ESSEL a , ... correlated with levels of carcass fat. The T allele was associated with fatter carcasses and the C allele with l...

Ngày tải lên: 09/08/2014, 18:21

... combination of fasting insulin and triglycerides [14], 'insulin sensitivity index' (ISI), was also used as surrogate a marker of insulin sensitivity. Power calculation and statistical analysis We ... were rand- omized to dexamethasone and 12 to placebo. Baseline plasma TNFα, ghrelin, leptin and adiponectin were com- parable among subjects in the dexamethasone an...

Ngày tải lên: 11/08/2014, 08:20

... that the replacement of a Pro with Leu or Ala in the hybrid analog P18 decreases its antibacterial activity and increases its hemolytic activity [39]. In general, introduction of a Pro near the ... of a Pro with an Ala maintained or decreased the antimicrobial activity but significantly increased the hemolytic activity. In addition, Oh et al. [38] reported tha...

Ngày tải lên: 23/03/2014, 10:21

... seven ligands of the diiron site in haemeryth- rins are amino acid residues each located within the four helices, and by diiron binding generating an intramolecular cross-linking of these helices, ... analysis modelled the putative haemerythrin to a four a- helix bundle, maintaining both the conserved haemerythrin positioning of the amino acid residues coordinating the...

Ngày tải lên: 07/03/2014, 17:20

... [12]. Vertebrate FABPs are involved in cellular fatty acid transport and utilization and compartmentalization of intracellular fatty acid storage, and also in fatty acid- induced regulation of gene expression ... ligand-binding studies with recombinant msCRABP showed high affin- ity binding for fatty acids and negligible interaction with RA and other retinoids, and he...

Ngày tải lên: 23/03/2014, 10:21

... present in the databases. The two proteins migrating at  50 and  55 kDa (bands A and B; Fig. 1A) were identified from their N-terminal sequences as similar to a- amylase (accession no. AAA17751) ... N-terminus of the proprotein in- frame and adjacent to the cleavage point of the yeast a- mating factor secretion signal encoded by the vector. In addition, a (Hi...

Ngày tải lên: 23/03/2014, 12:20

... (Markham Ontario, Canada). All other reagents were of analytical quality. Expression and purification of a- Syn and a- Syn 1)60 a- Syn and a- Syn 1)60 genes were subcloned into the pET- 3a vector, and ... China 2 Shanghai Institute of Materia Medica, Shanghai, China 3 Shanghai Institute of Nuclear Research, Shanghai, China 4 Bio-X Research Center, Shanghai Jiaotong Unive...

Ngày tải lên: 23/03/2014, 15:20

... spectra of the GGN4 analogues, including the native GGN4, showed a strong negative band near 200 nm and a weak and broad band around 222 nm, indicating a predominantly random-coil conformation with a ... would be advantageous to facilitate the amphipathic interaction between the peptide and the membrane surface, as the tryptophan side chain is amphiphilic in na...

Ngày tải lên: 31/03/2014, 09:20

... in ascites, and selected for developing a sandwich ELISA. The subclass of the MAb 2B1 and MAb 7A3 were identified as IgG 1 and IgG 2a , respectively, with kappa light chain. The MAb 7A3 and ... RT. After adding ABTS (KPL, USA) and 30 min’s incubation at RT, absorbance was measured at 405 nm. Sandwich ELISA The sandwich ELISA was performed on microtiter plates. Each...

Ngày tải lên: 07/08/2014, 18:21

... was assessed by the Ratingen score [28], a modification of the Larsen score. It evaluates 38 joints separately (all proximal inter- phalangeal and metacarpophalangeal joints, four sites in the ... for each radiograph by division of the Ratingen score by the disease duration at the time that the radiograph was taken. DNA isolation Blood samples containing EDTA as anti-c...

Ngày tải lên: 09/08/2014, 01:23