... concentra-tions for half-maximal inhibition (IC50) of BPA were35.7 nm for (275Ala)-ERRc, 27% of the bindingaffinity for the wild-type ERRc, and 990 nm for (316Ala)-ERRc, only approximately 1% of that for the ... (xxx ¼ gcg for Glu275Ala, cgg for Glu275Arg, gac for Glu275Asp, and ctg for Glu275Leu);5¢-TCCTTGGTGTCGTATACxxxTCTCTTTCA-3¢ (xxx ¼gcg for Arg316 fi Ala, aag for Arg316 fi Lys, ctg for Arg316 fi ... definitive for, particular functions.Evolutionary rationale for the major role of Arg316 in arresting the ligandWhen the amino acid sequences of the LBD of all theNRs were aligned to that of ERRc,...