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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

... clearly separated by the proximity of the cells to the joint space and the bone respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic cells. ... differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transferAndre F Steinert1,2, Benedikt Proffen1, Manuela Kunz1, Christian Hendrich1, ... compare the effects of BMP-4 and BMP-2 expression on chondrogenesis of primaryMSCs and to investigate whether levels and extent of hyper-trophy in vitro is influenced by the choice of transgene.Materials...
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Báo cáo y học: "Platelet-Activating Factor Antagonists Decrease Follicular Dendritic-Cell Stimulation of Human B Lymphocytes" pptx

... 217025,26clearly had an effect,decreasing cell proliferation (as measured by [3H]-thymidine incorporation) by 35% and the produc-tion of IgM and IgG by the isolated tonsillar cells by 45 to 75%. This ... lymphocytesfrom apoptosis.32,33A novel factor secreted by FDCs, 8D6, is not involved in rescue from apop-tosis and is not solely responsible for maintaining the growth of B-lymphocyte lines ... cells in culture and stainednegatively for CD20 and CD3 and positively forCD14 and VLA-4 by flow cytometry (data notshown). In addition, viability testing indicatedthat by day 6, all B cells...
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Báo cáo y học: "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model" pdf

... stimulus.Discussion In this study, we have demonstrated expression of CD47/IAP by human articular chondrocytes with predominant expression of the type 2 isoform. The type 2 isoform of CD47/IAP, ... stimulated and unstimulated cells. Tyrosine phosphorylation of a 125-kDa protein is increased after 1 minute of mechanical stimulation but this response is diminished by the presence of the anti-CD47/IAP ... provided the CD47/IAP-null and transfected cell lines and was involved in the analysis of results of the studies on these cells. DMSconceived of the study, participated in its design and coordi-nation,...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf

... the osteogenic differentiation of human mesenchymal stem cells (MSCs); and blockade of the activity of the BMP inhibitorincreases the rate of osteogenic differentiation of human MSCs in vitro. Methods Human ... modulatedextracellularly by several binding proteins, including noggin,gremlin, follistatin and chordin. The latter is a BMP antagonistthat was initially characterised in the Spemann organizer. It is a 120 ... differ-entiation of human mesenchymal stem cellsExpression of BMP-2 and chordin transcripts during osteogenic differ-entiation of human mesenchymal stem cells. (a) Temporal progression of bone morphogenetic...
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Báo cáo y học: "Apoptosis is not the major death mechanism induced by celecoxib on rheumatoid arthritis synovial fibroblasts" pps

... the experimental work and the analysis of the data and participated in the writing of the manuscript. VD per-formed several experiments. MH participated in the analysis of the study and in the ... role in controlling cell numbers by eliminating old cells, unnecessary cells, and unhealthy cells. Deregulation of apoptosis thus can lead to the survival and hyperproliferation of unwanted cells ... the writing of the manuscript. BC and JMparticipated in the design of the study and in the writing of the manuscript. All authors read and approved the manuscript.AcknowledgementsThis work...
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Báo cáo y học: "Eosinopenia is a reliable marker of sepsis on admission to medical intensive care units"

... acquisi-tion of data. AZ participated in the coordination of the study.AAZ participated in the design of the study, and performed the statistical analysis. RA conceived of the study, participated in the ... count and C-reactive protein level for discrimination of systemic inflammatory response syndrome and infectionEosinophil cell count and C-reactive protein level for discrimination of systemic inflammatory ... eosinopenia in the diagnosis of sepsis on admission to the ICU.• Eosinopenia is a good diagnostic marker in distinguish-ing between noninfection and infection in newly admit-ted critically...
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Báo cáo y học: "Placenta Percreta-Induced Uterine Rupture Diagnosed By Laparoscopy in the First Trimester"

... evaluated for the differential diagnosis such as appendicitis and hemoperitoneum. The gradual increase in the size of the uterus with advancing pregnancy can cause a delay in the diagnosis and appropriate ... lower uterine segment; however, in cases of uterine rupture during the first trimester, the site commonly affected is the fundus, as noted in our patient [7]. The uterine ruptures in the first ... such as in the case of our patient, laparoscopy can be helpful for the early di-agnosis of hemoperitoneum due to uterine rupture. In general, the area of placenta percreta -induced uterine rupture...
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Báo cáo y học: " Vgf is a novel biomarker associated with muscle weakness in amyotrophic lateral sclerosis (ALS), with a potential role in disease pathogenesis"

... one-week training period wherein they were intro-duced to the apparatus and handled by the operator daily. Testing was conducted during the last 4 hours of the day portion of the light cycle in an ... supports the hypothesis that a mechanism thorough which abnormal metabolism may ultimately influence ALS pathogenesis is through mechanisms involving reduced expression of Vgf. This evidence is of ... intensity, were made on digitized images by delimiting the cellular area of interest free hand, using predeter-mined criteria to define the region of interest. The immune intensity of...
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Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf

Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf

... observed, even in the presence of PPARcligand, confirming the role of the enzymatic activity of visfatin and the possibility that PPARc can modulateVisfatin induction by PPARc in human macrophages ... stillcontroversial, visfatin is thought to have insulinmimetic effects and, similarly to insulin, visfatinenhances glucose uptake by myocytes and adipocytes and inhibits hepatocyte glucose release in vitro ... visfatin expression and activity by small interfering RNA (siRNA) or a spe-cific inhibitor abolished the PPARc-mediated increase of NAD+.T. H. Mayi et al. Visfatin induction by PPARc in human...
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Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf

Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf

... pools of putres-cine, spermidine and spermine by down-regulating the activity of the polyamine biosynthetic enzymes a nd up-regu-lating the activity of the catabolic enzyme spermidine/spermine ... in the sonicate was deter-mined by measuring the synthesis of [14C]acetylspermidineafter incubation with [14C]acetyl coenzyme A and spermi-dine [20].Flow cytometry and data analysis Cells ... comparingdifferent cell lines [9,12,14]. In the polyamine m etabolicpathway, the induction of SSAT results in the acetylation of spermine and spermidine, which are subsequently oxidized by polyamine oxidase...
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