Báo cáo y học: "Expression of cartilage-derived morphogenetic protein in human intervertebral discs and its effect on matrix synthesis in degenerate human nucleus pulposus cells" potx

... resulting in decreased matrix production, particularly proteoglycan synthesis, and an increase in degradation of IVD matrix by locally produced matrix metalloproteinases (MMPs) and ADAMTS (a disintegrin and ... Interleukin-1 receptor antagonist delivered directly and by gene therapy inhibits matrix degradation in the intact degenerate human interverte- bral disc:...

Ngày tải lên: 09/08/2014, 14:22

... results of the morphometric analysis indicated induction of mitochondrial biogenesis by ectopic UCP1 in the unilocular adipocytes. The stimulatory effect of UCP1 on mitochondrial content and biogenesis ... expression in adipocytes. It may be also speculated that the effect of adrenergic system on mitochondrial biogenesis represents a compensation for decreased ef®ciency...

Ngày tải lên: 31/03/2014, 15:20

... augmentation of bone and bone-ten- don junction regeneration [3,4]. Because of the role of BMP-2 in bone formation, it is commonly found in bone and is generally absent in tendon. We hypothesized ... and Kai Ming Chan 1,2 Address: 1 Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, PR China and 2...

Ngày tải lên: 20/06/2014, 04:20

... differentiation by increasing BMP-2 bioavailability. Future characterization of all the inhibitors and technological advances in suppressing their expression (including gene therapy, small interfering RNA ... problem of inadequate bone healing. In addition, high concentrations - at high cost - of single growth factors with a range of questionable carriers is the current paradigm...

Ngày tải lên: 09/08/2014, 10:23

... permeability and retention (EPR) mechanism (7). However, the use of conventional vesicles cannot fully overcome their binding with serum components and uptake by mononuclear phagocyte system (MPS). ... following re-dissolution after freeze-drying, was separated by mini-gel columns of Sephadex-G50 prepared in advance. In brief, incubation mixtures of 200μL containing unload...

Ngày tải lên: 26/10/2012, 08:57

... proteases in RA: MMP1 and MMP3 are secreted on stimulation by inflammatory cytokines; they degrade key components of cartilage and bone matrix [6]. MMP1 expression occurs in cells of the invading front ... determination of the concentration of MMP1 and MMP3 and stored at –80°C (see below). Genotyping MMP1 and MMP3 promoter polymorphisms were determined by single-stra...

Ngày tải lên: 09/08/2014, 01:23

... induction of AP [9]. Figure 2 Hematoxylin-eosin staining (a) and indirect immunofluorescence for collagen types I (b) and II (c) in comparable areasHematoxylin-eosin staining (a) and indirect immunofluorescence ... tendon, and always close to vessel or duct structures and the acinar elements in the acromial portion (Figure 4). Expression of mRNA for bone morphogenetic pro...

Ngày tải lên: 09/08/2014, 08:22

... present in inclusion body. In order to recover its function, the fusion protein in inclusion body was refolded in urea gradient refolding buffer. And then, the refolded protein was incubated ... treat type 2 daibetes by improving both insulin resistance and insulin secretion deficiency. However, we only ob- served the glucose-lowering effect of the whole fusion pro...

Ngày tải lên: 25/10/2012, 11:10

... omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification and Statistical analysis ... applying 0.01% diaminobenzidine tetrahydrochloride and 0.03% H 2 O 2 . Sections were counterstained with Meyer’s haematoxylin and mounted in Permount TM . Negative controls c...

Ngày tải lên: 03/11/2012, 09:54

... coding for the subunits A and B of V-ATPase. The intracellular localization of these isoforms and the combination of the isoforms is yet to be determined. We are now trying to express fusion proteins ... cDNA and genomic cloning of sugar beet V-type H 1 -ATPase subunit A and c isoforms: evidence for coordinate expression during plant development and cooridinate induction in...

Ngày tải lên: 08/03/2014, 23:20