Báo cáo y học: " Increased expression of matrix metalloproteinase-10, nerve growth factor and substance P in the painful degenerate intervertebral disc" pot

... differences in the cata- bolic pathways between painful and pain-free IVD degeneration. While this study focused on gene and protein expression profiling, it emphasises the importance of MMP-10 in symptomatic ... to examine the gene and pro- tein expression of MMP-10 in histologically normal human IVD and compare it with that of both non -painful and painfu...

Ngày tải lên: 09/08/2014, 14:22

... San Leandro, CA, USA), and the inten- sity of the L-PGDS bands was normalized by dividing them by the intensity of the β-actin band of the corresponding sample. 11β-PGF 2 α and PGD 2 assays The ... (IL-1β)-induced upregulation of lipocalin-type prostaglandin D synthase (L-PGDS) expressionEffect of prostaglandin D 2 (PGD 2 ) on interleukin-1-beta (IL-1β)- induced up...

Ngày tải lên: 09/08/2014, 01:22

... equally to this work. YL carried out data analysis and interpretation, and the study design, and assisted in manuscript preparation. PYL participated in date analysis and interpretation, and assisted ... infiltration of these cells to the sites of IC deposition in the kidney [48]. Since IFNα and IFNγ also stimulate the production of monocyte attractants such as...

Ngày tải lên: 09/08/2014, 01:22

... contributions US and NS performed the Affymetrix chip analysis. US, NS and CP analysed the MMP, cathepsin and cytokine expres- sion by real-time PCR. MH performed the zymography, the MMP activity assay and the ... recently reviewed by Yan and Sloane [34]. However, the expression and function of most of the cathepsins in RA are unknown. Our results support th...

Ngày tải lên: 09/08/2014, 06:22

... San Leandro, CA, USA), and the inten- sity of the L-PGDS bands was normalized by dividing them by the intensity of the β-actin band of the corresponding sample. 11β-PGF 2 α and PGD 2 assays The ... indicate increased expression and activity of L-PGDS in OA tissues. Figure 2 Expression of lipocalin-type prostaglandin D synthase (L-PGDS) protein in healthy...

Ngày tải lên: 09/08/2014, 13:22

... equally to this work. YL carried out data analysis and interpretation, and the study design, and assisted in manuscript preparation. PYL participated in date analysis and interpretation, and assisted ... infiltration of these cells to the sites of IC deposition in the kidney [48]. Since IFNα and IFNγ also stimulate the production of monocyte attractants such as...

Ngày tải lên: 09/08/2014, 13:22

... fam- ily of proteolytic enzymes containing a metal in their organic structure playing key roles in degradation of pro- teins in extracellular matrix and in tissue remodeling through complicated ... family of proteolytic enzymes, known as metzincin super-family. Their catalytic action is the degradation, mainly in neutral pH environment, of all proteins of the extra...

Ngày tải lên: 10/08/2014, 10:20

... and apoptosis, supporting the hypothesis that c-Fos and c-Jun act coordinately to increase the expression of BimEL. Increased c-Fos levels are therefore critical for BimEL expression and apoptosis ... as PTP1B [11], MKP3 [12], and LMW-PTP [13]. The cysteines in the active sites of these phosphatases are easily inactivated by ROS, resulting in activation of the...

Ngày tải lên: 06/03/2014, 00:21

... 2002 20 lL of the enzyme/inhibitor mixture to 180 lLof substrate (4NPGlc), incubating for 30 min at 37 °C, and measuring the rel ease of 4NP. Protein assays and protein sequencing Total protein in crude ... liver. Furthermore, we investigated the speci®city of the human CBG with r espect to the glycone and aglycone moieties, and in particular characterized the ef...

Ngày tải lên: 08/03/2014, 16:20

... removed completely. The pellet was broken by gently tapping on the bottom of the tube and placed in a 37 °C water-bath. Prewarmed 0.5 mL of 50% poly(ethylene glycol) 1500 (Boehringer Mannheim, Germany) was ... and hence predefined molar ratios. The result presented here show that the fusion event typically does not alter the expression level of the recombinant prote...

Ngày tải lên: 18/03/2014, 01:20