Báo cáo y học: "Immunization with an immunodominant self-peptide derived from glucose-6-phosphate isomerase induces arthritis in DBA/1 mice" pot

... arthritis induced by peptide as compared with arthritis induced by G6PI protein. In our hands, peptide- induced arthritis occurs somewhat delayed and with lower incidence and clinical severity than ... (JES6-5H4)-fluorescein isothiocyanate, anti-IL-6 (MP5-20F3)-fluorescein isothiocyanate, anti-IL-10 (JES5-16E3)-APC, and anti-RANKL (IK22/5)-phycoerythrin were purchased from ebios...

Ngày tải lên: 09/08/2014, 14:22

... microneme protein P52 (PBANKA_100220; also known as P36p) [11]. Here, we sought to quantify memory lymphocyt es, elicited b y immunizations with p52 - GAP, which may play a r ole in maintenance in the ... liver stage infection and immunizations assays, organs collection, non-parenchy mal cells extraction and staining, FACS, data analysis and participated both in the study design and...

Ngày tải lên: 11/08/2014, 08:21

... has multiple hyperpigmented nevi all over his body, short feet with pes cavus and claw toes. At age of 30 years, cytogenetic and molecular cytogenetic analysis revealed a karyotype of 50,XY,+min(6)(:p11.1-> ... 30-year-old Spanish Caucasian man; the third child from healthy and non-consanguineous parents. The first child was a healthy boy a nd the sec- ond child was also a boy who died...

Ngày tải lên: 11/08/2014, 03:21

... (Tokyo) 16, 769–774. 15. Anastasi, A., Bertaccini, G. & Erspamer, V. (1966) Pharmacolo- gical data on phyllokinin (braykinyl-isoleucyl-tyrosine-0-sulphate) and bradykinyl-isoleucyl-tyrosine. ... may provide an insight into the apparent absence of a circulating kallikrein-kinin system in anuran amphibians [17,18,23]. The high degree of primary structural variability of bradykinins in...

Ngày tải lên: 21/02/2014, 15:20

... expressed in Escherichia coli and in Pichia pastoris, and analyzed for monoclonal antibody and IgE antibody binding by ELISA and/or immunoblotting. Optimal rBla g 1 expression was obtained using methanol- inducible ... eight Mut s transformants expressing one and two duplexes, respectively, were obtained from the KM17 strain grown on media containing 100 lgÆmL )1 zeocin. Using a single...

Ngày tải lên: 08/03/2014, 23:20

... colony-stimulating factor) by human monocytes [34]. The uPA-induced arthritis may be mediated, besides its proteolytic and cytokine triggering effects, by a direct interaction with the uPAR, a high affinity ... full serine protease activity and the amino-terminal fragment interact- ing with the uPAR, appeared to be less efficient both in giving rise to the cytokine production and in...

Ngày tải lên: 09/08/2014, 01:21

... (WNT8B, SEC31B, NDUFB8, and HIF 1AN) , they are no longer syntenic as a group in teleost. WNT8B and HIF 1AN are in synteny at one locus, and SEC31B and NDUFB8 in the other, showing a 'split-up' ... the approach taken by Synorth can aid in adding missing gene annotation and detecting likely cases of genome mis-assem- bly. Returning to PAX2 GRB as an example (Figure 5), we...

Ngày tải lên: 09/08/2014, 20:20

... co-stained for DNA with Propidium Iodine (Upper left panel) and stained with anti-GFP antibody and secondary FITC labeled antibody (Upper right panel). Lower left panel – tissue image in reflected ... Immunostaining for actin used as control. C: Expression of PRXV is moderately up-regulated in the A549 cells by P. aeruginosa PAO1 and by co- culture with RAW 264.7, with and with...

Ngày tải lên: 11/08/2014, 08:21

... 7-chloro-4-nitrobenzo-2-oxa- 1,3-diazole, ethacrynic acid, p-nitrobenzyl chloride, dehydroascorbate, bromosulfophthalein, Evan’s blue, hem- atine, and p-chloranil were obtained from ICN; hydroxy- ethyl disulfide (HEDS) and vinylene trithiocarbonate ... expressed more in parasitic stages than in free living stages during the S. mansoni life cycle. Characterization of recombinant SmGSTO en...

Ngày tải lên: 21/02/2014, 01:21

... iodide staining, according to the method of Nicoletti and colleagues [19], and by the binding of FITC- conjugated Annexin V, using the Apoptest binding kit, contain- ing annexin V-FITC and binding ... an essential role in cytokinesis in yeast and mammalian cells [24]. Nedd5 is predominantly expressed in the nervous system and may contribute to the formation of neurofibrillary tangles...

Ngày tải lên: 09/08/2014, 06:23