Báo cáo y học: "Degradome expression profiling in human articular cartilage" potx

... predominantly intracellularly [26,27]. Cathepsin H, cathepsin C, cathepsin F, cathepsin W, cathepsin B and cathepsin S are also increased in expression in OA tissue Table 3 Fold change and threshold cycle ... identified), particularly in the nervous system since the ADAM22 null mouse displays ataxia and peripheral nerve hypomyelination [20]. Following ectodomain shedding, some transme...

Ngày tải lên: 09/08/2014, 14:22

... by genes encoding interleukins, chemokine receptors and ligands, and by those involved in antigen recognition and processing. Keywords: DNA expression array, differential gene expression, inflammation, ... of molecular interactions in an inflamed joint, deduced from gene expression studies in both RA and its corresponding animal models, involves pro- teins participating in immuni...

Ngày tải lên: 09/08/2014, 06:22

... significantly overexpressed at baseline in poor responders are induced by several cytokines in FLSs, thereby suggesting a role for these cytokines in the resistance to TNF blockade in RA. ANOVA: analysis ... thereby preventing disease progression in these patients and saving unnecessary costs. TNF antagonists interfere with many pathways involved in RA synovial inflammatory proces...

Ngày tải lên: 09/08/2014, 14:20

... arthritic inflammation was induced 14 days after intra -articular injection (designated day 0) by intravenous injection of 200 µg PG-PS. This resulted in monoarticular arthritis involving the joint ... homogenized joints can provide useful information about dysregulated genes, not only in synoviocytes but also in other, neighbouring cells (myocytes, osteocytes and chondrocytes) that ma...

Ngày tải lên: 09/08/2014, 06:22

... photosynthesis on our planet. Morpholog ica lly, they exhibit different shapes and symmetries, the centric diatoms being radially symmetric and the pennates dis- playing bilateral symmetry. In spite ... fused into a consensus sequence using PHRAP [55]. Sequence analysis The complete set of cDNAs was subject to a preliminary analysis as previously described in [22], with a variation in o...

Ngày tải lên: 09/08/2014, 20:22

... BCL-2 (Figure 2), may enhance their binding to BCL-2, thereby inhibiting the anti-apop- totic function. Thus, we analyzed the BCL-2 protein expression level by flow cytometry in Molt-4 cells at ... phosphoprotein 1 (SPP1), cAMP- response element binding protein (CREBBP), and b2- MG were determined by real-time PCR using a SYBR Green I qPCR Master Mix kit [15]. Flow cytometry assay Cells f...

Ngày tải lên: 10/08/2014, 21:23

... in human RBCs encoding initiation, activation and regulation of transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD finger- DNA-binding proteins, cysteinyl, lysyl-tRNA ... Transcript profiling of human platelets using microarray and serial analysis of gene expression. Blood 2003;101:2285-2293. 16. Weyrich AS, Lindemann S, Tolley ND et al. Change in p...

Ngày tải lên: 03/11/2012, 10:58

... GPVI, glycoprotein VI; PtdIns3K, phosphatidylinositol 3-kinase; PVDF, poly(vinylidene difluoride); PKC, protein kinase C; PP1, (4-amino-4-(4-methylphe- nyl)-7-(t-butyl)pyrazola[3,4-d]pyrimidine); ... using GST fusion protein con taining the RAS-b inding domain o f RAF. Precipi- tated proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig (top). Proteins...

Ngày tải lên: 08/03/2014, 16:20

... especially focusing on clinically relevant endpoints like radiation induced cell death, proliferation, migration and metastasis. Introduction MicroRNAs are small non-coding RNAs of typically 20 - 22 ... response in vivo in C. elegans and in vitro in human breast cancer cells. Oncogene 2009, 28:2419-2424. 12. Maes OC, An J, Sarojini H, Wu HL, Wang E: Changes in MicroRNA Expression...

Ngày tải lên: 09/08/2014, 09:20

... passage 7-9 into T75 cm 2 flasks on day 0 and cultured in DMEM/10% FCS. The dose of bleomycin sulphate was previously determined by cell cycle analysis using fluorescence activated cell sorting (FACS) ... using an Affymetrix GeneChip Instrument System and hybridised to HGU133plus2 arrays. Arrays were scann ed in an Affymetrix GeneChip Scanner 3000 system. Data pre- processing was carried...

Ngày tải lên: 09/08/2014, 09:20