Báo cáo y học: "Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzyme" pot

... 11 No 2 Research article Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzymes Vincent Goëb 1 , Marlène Thomas-L'Otellier 2 , ... antigens, enzymes of the glycolytic family and molecular chaperones are also targeted by the early untreated RA autoAb response. For som...

Ngày tải lên: 09/08/2014, 13:22

... of analysis and measurement time. Identifying proteins by mass spectrometry Intact proteins are difficult to identify by MS because their sequence cannot be obtained by fragmentation and so ... Analysis of complex peptide mixtures by LC-MS 2 . Peptide mixtures are resolved by liquid chromatography, ionized through electrospray and resolved by MS 1 . Selected peptid...

Ngày tải lên: 09/08/2014, 20:20

... lipopolysaccha- rides, and cytokines, especially interleukin-1 (IL-1), in cer- tain cell types involved in inflammatory processes, e.g. fibroblasts and macrophages [15,16]. Crofford and co- workers ... LB, Lipsky PE: Cyclooxygenase in biology and disease. FASEB J 1998, 12:1063-1073. 13. Yokoyama C, Tanabe T: Cloning of human gene encoding pros- taglandin endoperoxide synthase a...

Ngày tải lên: 09/08/2014, 01:23

... chromatog- raphy, immunologically and by high-performance liquid chromatography. The biological activity of (2–5)A oligo- mers was verified by inhibition of the protein synthesis in rabbit reticulocyte lysate ... they contain proliferating cells and their interior is surrounded by an almost complete single-cellular layer of epithelial-like cells, pinacocytes; the cells inside the p...

Ngày tải lên: 21/02/2014, 15:20

... interleukin-8 (IL-8) levels in joint fluids of patients with rheumatoid arthritis and the induction by IL-8 of leuko- cyte infiltration and synovitis in rabbit joints. Lymphokine Cytokine Res 1991, 10:245-252. 32. ... IL-17-induced inflammation and joint damage, implying a pathway independent of IL-1 [40]. The interaction of the cytokines IL-1β, IL-17, and TNF-α sus- tained...

Ngày tải lên: 09/08/2014, 01:22

... detected by silver staining on polyacrylamid gel. Statistical Analysis Allele frequencies were determined by gene counting and compared using the א² test. The data was analysed using SPSS ... PCR condition were: 96°C for an initial 5 minutes (min.) followed by 96°C 1 min., 65°C 1 min., 72°C 1 min. for 43 cycles and final extension at 72° for 10 min. MS-PCR created two fragmen...

Ngày tải lên: 31/10/2012, 17:03

... quantified by in vitro binding autoradiography. Circulating leptin levels, following a chronic forced swimming stress, were measured by radioimmunoassay (RIA). Data were statistically analyzed by analysis ... improving their cognitive abilities. This effect on behavior could be mediated by the handling-induced increase in hippocampal GR/MR ratio and BDNF levels. The handling-ind...

Ngày tải lên: 08/08/2014, 21:20

... activity and high activity did not show any difference. We could not find any correlation with sex and age (whole group/division into two patient groups ≤ 65 years and ≥ 66 years, P = 0.32), whereby ... essential role in osteoclast function and in the degradation of protein components of the bone matrix by cleaving proteins such as collagen type I, collagen type II and osteone...

Ngày tải lên: 09/08/2014, 06:22

... and/ or draining lymph nodes, for instance in tolerated allografts [28], in Langerhans islets and pancreatic lymph nodes in inflammation-induced dia- betes [29], in chronically inflamed skin in a Leishmania infection ... responses to the inducing antigen (methylated bovine serum albumin; mBSA) and autoantigens, and to an exacerbation of arthritis, as indicated by clinical...

Ngày tải lên: 09/08/2014, 06:22

... patients carrying a PTPN22 1858T allele was 5.5 years younger com- pared to patients without such a genotype (CT and TT geno- type 45.5 years versus CC genotype 51.0 years; p = 0.029), in line with ... (2.51–7.96) Values are the frequency in percent with absolute number of genotypes given in parentheses. Rheumatoid arthritis (RA) patients and controls were stratified by se...

Ngày tải lên: 09/08/2014, 08:22