Báo cáo y học: "Collagen-specific T-cell repertoire in blood and synovial fluid varies with disease activity in early rheumatoid arthritis" pot

... antigen-specific T-cell repertoire is spontaneously enriched in synovial fluid. The specific T-cell repertoire in the periphery was modulated by therapy and decreased with the remission of the disease. ... prominent role in joint inflammation. The T-cell mediated self-reactivity CDR3: third complementarity-determining region; CFSE: carboxyfluorescein diacetate succinim...

Ngày tải lên: 09/08/2014, 13:22

... of IFN-inducible chemokines in peripheral blood cells were closely associated with disease activity and organ damage in SLE, and may be useful in disease monitoring and prognostication. Materials and methods Patients ... 2 Association of chemokine and IFN scores with disease activity in SLE patientsAssociation of chemokine and IFN scores with disease a...

Ngày tải lên: 09/08/2014, 13:22

... changes in car- diac index during PLR were examined. An increase in cardiac index of 10% or more during PLR predicted an increase in car- diac index following VE of 15% or more with a sensitivity of 91% ... significantly correlated both during PLR and following VE [30]. Lafanechère and colleagues examined 22 intubated and fully sedated patients with an esophageal Doppler monit...

Ngày tải lên: 25/10/2012, 10:06

... poly- (b-lysine) chain of nourseothricin must be synthesized by an NRPS-like system in a mechanism that uses NspA, a stand-alone b-lysine adenylating enzyme (A-domain) and NspB, a b-lysine binding ... of the genes of actinomycin synthetase I and a 4-methyl-3-hydroxyanthranilic acid carrier protein involved in the assembly of the acylpeptide chain of actinomycin in Streptomyces. J. B...

Ngày tải lên: 17/03/2014, 17:20

... aminoethylcarbazole staining (red) for active caspase-3 (hematoxylin-counterstained) before (a) and after (b) treatment and diaminobenzidine staining (brown) for TUNEL (hematoxylin-counterstained) ... were then stained with mouse IgG1 allophycocyanine-conjugated anti- CD3 antibody (555335; BD Biosciences) and incubated with Annexin V and 7-amino-actinomycin D (7-AAD) as specified...

Ngày tải lên: 09/08/2014, 01:22

... or oxygen deprivation, inhibited synthesis of type I and type II collagen, both in monolayer and alginate cultures. Conclusion This study demonstrates that factors present in serum interact with ... Ltd., Paisley, UK). Freshly isolated NP cells are chondrocytic in phenotype, but during monolayer cul- ture, they become adherent and fibroblast-like in morphology and enter the...

Ngày tải lên: 09/08/2014, 10:23

... were then stained with mouse IgG1 allophycocyanine-conjugated anti- CD3 antibody (555335; BD Biosciences) and incubated with Annexin V and 7-amino-actinomycin D (7-AAD) as specified by the manufacturer ... IgG1 phycoerythrin-conjugated anti-CD3 antibody (HIT3a; BD Biosciences), followed by incu- bation with Annexin V (TA5532; R&D Systems, Minneapolis, MN, USA) and flow cytomet...

Ngày tải lên: 09/08/2014, 13:22

... away from home 7(21.2%) and being busy with other things 7(21.2% ) and the rest reasons included simply forgetting, having no food to take with the medication, fear of side effect and feeling ... 9(27.3%), being away from home 7(21.2%) and being busy with other things 7(21.2%) and the rest reasons included sim- ply forgetting, having no food to take with the medica- tion, fe...

Ngày tải lên: 10/08/2014, 05:21

... light stimulus. Methods General housing and care Mice were housed singly in optically clear polycarbonate cages (L × H × W: 11 × 8 × 7 in) with approximately 250 ml of Sani-chip ® (Harlan Teklad) bedding. They were acclimated ... nm only. Care in the darkroom consisted of ten min per day and each mouse was inspected for less than a minute. Daily visits occurred at random times bet...

Ngày tải lên: 10/08/2014, 09:20

... the monitoring of general motor activity of 12 mice housed individually in standard breeding cages. Methods Electronic system for the recording of locomotor activity The locomotor activity of the ... left in their breeding cages, thus avoiding a change of environment and the resulting changes in exploratory activity [31]. The computerized recording system also permits very lon...

Ngày tải lên: 10/08/2014, 09:20