Báo cáo y học: "Identification of intra-group, inter-individual, and gene-specific variances in mRNA expression profiles in the rheumatoid arthritis synovial membrane" pot

... liverFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●YYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYY Y YYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYY Y YY Y YYYYYYYYYYYYYYYY Y YYYYYYYYYWetterbom et al. Genome Biology 2010, 11:R78http://genomebiology.com/2010/11/7/R78Page ... 133 in brain and n = 39 in liver) could notbe located in either the human or the macaque genomes,thus indicating sequence gain in the chimpanzee lineage.For the remaining part of novel TRs the ... kb upstream of the other exons). Removal of exon 5 in human causes a shift in the reading frame and introduces premature termination codons in the pre-dicted protein sequence. Since the novel...

... suppression of MM12.10 labeling after UIC2 binding) may be intrinsic to the normal catalytic cycle, or, alternatively, the CsA-typedrugs may induce a special conformation adopted by Pgponly in the ... Thesefindings may be interpreted suggesting that changes in the UIC2 reactivity of Pgp are brought about by nucleotidebinding and release [18,30], or assuming that the hydrolysis of ATP is the ... respectively. Cells were trypsinized2–3 days prior to the experiments and maintained withoutvinblastine or doxorubicin until use. The cells were occa-sionally checked for mycoplasma3by the mycoplasma...

... none of them could name it. Interestingly, while the interviewees could not name the disease that was infecting the crabs in the region, all of them could identify the major clinical symptoms of ... the period just prior to the arrival of the disease. Interestingly, none of the crab harvesters knew the official name of this disease. According to most of the interviewees (about 70%), the ... of the use of “redinhas” in the region, although none of the interviewees claimed to use them. When asked about the best period for collecting crabs all of the interviewees indicated that the...

... this study. The presence of dyspeptic com-plaint and its severity calculated by presence of ab -dominal pain, abdominal pain with hungry and an -xiety, abdominal distension, nausea, vomiting, ... history, and certain laboratory data may guide the choice of which endoscopic in -vestigation to perform first in patients with IDA, the- reby, potentially reducing the frequency of negative Int. ... predict the outcome of up-per/lower endoscopy in outpatients with iron defi-ciency anemia. The aim of our study was to investi-gate the incidence of GI pathological findings in symptomatic and...

... determine the identity of the interacting proteins. 3. Results and Discussion 3.1 Principle of Split-Ubiquitin Screening System Studies have shown that the use of yeast two hybrid system, which is ... Construction of cDNA Library in S. cerevisiae DSY-2 Strain The cDNA library was prepared by transforming the yeast S. cerevisiae DSY-2 strain, in which the cDNA mixture was combined with pDL2-XN ... proteins that interact with HBsAg and thereby contributing to HBV morphogenesis. Using the yeast split-ubiquitin system, a number of cellular membrane proteins have been isolated in this study. These...

... isobutyl-methylxanthine and indomethacin. About10–30% of the OC cells were reproducibly inducedtoward the adipogenic lineage as early as 2 weeks afterinduction (Fig. 5g,5h). Using PCR, the expression of peroxisome ... and analyzed the kinet-ics of cultivation and the potential for differentiation. The data analysis after sorting is exemplarily shown for onepatient in Fig. 4. The gates used for cell sorting ... staining. The distribution of OC cells in forward/side scatter exhib-ited no difference between isotype and antibody staining. Amaximum of 2% positive cells by staining with isotype anti-body...

... summa-rized in Fig. 2, including intra-assay and interassayvariability (Fig. 2a), linearity (Fig. 2b), and ROC analysis,PPV, NPV and efficiency (Fig. 2c).To determine the cutoff, the focus was ... shown). The absence of inhibition can beexplained by the variety of different anti-Sm antibody sub-populations and by the variety of corresponding epitopes.Recently, two polyclonal antibodies (SYM10, ... quotient.ResultsEpitope fine mapping of the carboxyl-terminal extensions of SmD1 and SmD3To evaluate the effect of arginine dimethylation on the anti-genicity of SmD1 and SmD3 and to localize relevantepitopes...

... integrins to the cytoskeleton and recruit sig-naling molecules [33]. A new study reported that a kinectinisoform lacking a major portion of the kinesin-binding domainis very probably the most ... throughout the study. SLC and EMT con-ceived the study, participated in the design and helped in the analysis of the data. EKLC participated in the design of the study, interpreted data and helped ... two-hybrid screen studies from several laboratories have revealed the interaction of the Rho family of GTPase with kinectin, and have shown the functional links among RhoG, kinectin and kinesin,...

... 6Localisation of citrullinated proteins in synovial membranesLocalisation of citrullinated proteins in synovial membranes. (a) Immunostaining of citrullinated proteins by the anti-modified citrulline kit ... confirming the identity of the autoantigen.Conversion of arginine to citrulline results in a mass increase of 0.984 Da and a loss of the positive charge from the sidechain, resulting in a significant ... patientswith RA. The identity of the antigen was established by massspectrometry, and the sites of nine citrulline residues within the protein were determined by tandem mass spectrometry.Further confirmation...

... RNA and poly (A)+ RNA from jointsAfter removal of the skin and muscle, portions of the leg con-taining the knee, ankle, and finger joints were rapidly frozen in liquid nitrogen. Frozen joints ... synovial lining and fluid of the periarticular spaces [2]. The infiltrating cells expressadhesion molecules and produce a variety of inflammatorycytokines and chemokines to contribute to the complex ... chemokines, and cytokines isfrequently seen in the joints of patients with RA [27,29], indi-cating that the gene expression profiles obtained from theseRA models well represent those of patients...