... 90 min. Sections werewashed in PBS, incubated in biotinylated secondary anti-body for 60 min, washed again in PBS, and then incu-bated in an avidin-rich solution for 60 min. After a finalwash, ... Bonnefoy R, Pasdeloup M,Mouret C, Bigard X, Peinnequin A, Freyssenet D: Ectopic expression of myostatin induces atrophy of adult skeletal muscle by decreasingmuscle gene expression. Endocrinology ... range of factors in skeletal muscle (e.g., anti-oxi-dant therapy or strength training p aradigms) may provemost beneficial to maintain skeletal muscle mass.The IL-6 family of cytokines, including...