Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx

... article Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular ... analysis and immunohistochemical identifi- cation of ECM components. Immunohistochemistry showed that expression of collagen type I,...

Ngày tải lên: 09/08/2014, 10:23

... spatially distinct cellular zones, with the superfi- cial layer resembling hyaline cartilage, and immunohisto- chemically detectable collagen type II and cartilage proteoglycan link protein within ... for cell-based tissue engineering and regeneration. This review focuses on the biology of MSCs, including their differentiation potentials in vitro and in vivo, and the a...

Ngày tải lên: 09/08/2014, 01:21

... trehalose oligosaccharides (Eur. J. Biochem. 269) 3143 The origin of these cytosolic oligosaccharides is not known. They could be synthesized in the cytosol by glycosyltransferases which add glucosyl ... 5% to precipitate the protein. After removal of the protein by centrifugation, the trichloroacetic acid was removed by extraction with diethyl ether, and then alcohol was added...

Ngày tải lên: 22/02/2014, 07:20

... scan. The reversibility of the thermal transitions was checked by examining the reproducibility of the calorimetric trace in a second heating of the sample immediately after cooling from the first ... donor:hydrogen-peroxide oxido- reductase) are enzymes that are widely distributed in the living world and that are involved in many physiological processes, including abiotic a...

Ngày tải lên: 31/03/2014, 15:20

... signaling via Tie2 [68]. Figure 4 Tie1-751 interacts with Tie1 and Tie2Tie1-751 interacts with Tie1 and Tie2. (a) Specific binding of 125 I- Tie1-751(6His) to human umbilical vein endothelial cells ... manuscript. Figure 5 Inhibition of murine collagen- induced arthritis by Tie1-751Inhibition of murine collagen- induced arthritis by Tie1-751. On the day of arthritis onset,...

Ngày tải lên: 09/08/2014, 10:23

... efficiency of masking repetitive elements during the capture probe design and of reducing their nonspecific hybridization by adding Cot-1 DNA in the solution-hybridization step by determining how many ... vari- ant-detection sensitivity was only 64% to 80% within the exonic sequences, likely because of insufficient coverage uni- formity. In this study, we further assessed the soluti...

Ngày tải lên: 09/08/2014, 20:20

... have migrated into the tissue away from the injection site, but the majority of the MSCs remained in a cellular cluster within the injection site (Figure 1). Cell viability/metabolic activity Few ... post injection (Figures 4 and 5b). Aggrecan matrix staining within the vicinity of the injected cells was also observed 1 week following injection, and the intensity of t...

Ngày tải lên: 09/08/2014, 01:22

... prolactin and growth hormone from pituitary cells in vitro [7]. Since then, various biological activities of TS such as inhibition of acetylcholine esterase activity [8] and induction of apoptosis in ... various stimuli was quickly oxidized to nitrite. Nitrite in the culture medium was measured colorimetrically using Griess reagent (1% sulfa- nilamide, 0.1% N-1-naphthyl-ethylenedi...

Ngày tải lên: 22/02/2014, 04:20

... live and divide well either with Glu-tubulin or with tubulin in which tyrosine is replaced by nitrotyrosine (present study). However, we cannot yet exclude the possibility that nitrotyrosination ... succes- sive cycles of nitrotyrosination, de-nitrotyrosination, and re-nitrotyrosination (Fig. 3). This indicates strongly that substitution of C-terminal tyrosine by nitrotyrosine is re...

Ngày tải lên: 17/03/2014, 10:20

... all skin types, the phaeomelanin/eumelanin ratio is higher in individuals with red hair and fair skin [18]. Activation of MC1R by its agonists promotes the switch from phaeo- to eumelanogenesis, and ... determinant of agonist binding. Within this region, results by others have shown that removal of the negative charge at position 94 strongly diminishes agonist binding affinity [3...

Ngày tải lên: 17/03/2014, 10:20