Báo cáo y học: "Caveolin-1 expression and stress-induced premature senescence in human intervertebral disc degeneration" docx

... article Caveolin-1 expression and stress-induced premature senescence in human intervertebral disc degeneration Sarah Kathleen Heathfield 1 , Christine Lyn Le Maitre 2 and Judith Alison Hoyland 1 1 Tissue ... Hoyland JA, Freemont AJ: Interleukin-1 receptor antagonist delivered directly and by gene therapy inhibits matrix degradation in the intact degenerate human in...

Ngày tải lên: 09/08/2014, 10:23

... cystatins, the kininogens, are larger proteins containing three tandemly repeated type 2-like cystatin domains. Type 2 cystatins identified in higher animals include cystatins C, D, S, SA, and SN [2], ... are readily secreted in mammalian [20] and insect [5] cells, respectively. Cystatin F is an unusual type 2 cystatin in that it is a glycoprotein [5,6], and it is known that many p...

Ngày tải lên: 21/02/2014, 01:21

... reactions were incubated at 30 °Cfor 90 min and the products analyzed on 4.5% nondenaturing PAGE. Following staining in Coomassie blue and destain- ing, the gels were soaked in Amplify solution (Amersham), dried ... symmetry and is composed of seven to nine distinct polypeptides (designated as a, b, c, etc.) [8] whereas GroEL has a sevenfold symmetry and is made of a single polype...

Ngày tải lên: 23/03/2014, 21:20

... nitroalkane by NaoA. Keywords: enzymatic properties; expression; gene cloning; nitroalkane-oxidizing enzyme; Streptomyces. Nitroalkane compounds are widely used in chemical indus- try as intermediates, ... Electro- phoresis Power Supply and a Mini IEF Cell (Bio-Rad). Protein standards of different pI were as follows: amylo- glucosidase, pI 3.6; b-lactoglobulin A, pI 5.1; myoglobin, pI 6....

Ngày tải lên: 31/03/2014, 08:20

... an increase in Cyp7b activity and Cyp7b mRNA level in synovial biopsies [9]. Recently, we reported that Cyp7b mRNA expression and Cyp7b activity are present in FLS from patients with RA [10]. In ... Cyp7b expression. Use of the MAPK inhibitors PD98059 and SB203580 did not result in convincing changes in TNF-α-induced Cyp7b mRNA expression. We then determined Cyp7b enzy...

Ngày tải lên: 09/08/2014, 07:20

... MMPs, proinflammatory cytokines and chemokines [13]. There is increasing evidence that SFs are key players in the pathogenesis of RA by invading and eroding hyaline cartilage. SFs, activated locally, ... growth factor, insulin-like growth factor-binding proteins), morpho- gens, enzymes, numerous cytokines and chemokines, inter- leukins, and so forth [52]. It can be hypothesized that...

Ngày tải lên: 09/08/2014, 14:20

... affected initially, and sepsis leads to severe injury in lung tissue [5]. It has been shown that pericytes in lung tissue produce proinflammatory cytokines in response to lipopolysaccharide ... and clinical studies in animal models and human beings are needed to un- derstand molecular elements, ligand and receptors, involved in the mode of action of ozone and HBO....

Ngày tải lên: 25/10/2012, 11:18

... an invariantpartofallthreeclassesoftheplantperoxidase superfamily [44]. This motif (shown in grey shading in Fig. 1) includes Ser96 and Asp99 located at the beginning of helix D and connecting to the following long loop in a tight hydrogen bonding network ... promoter activity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C...

Ngày tải lên: 21/02/2014, 01:21

... aliquot of Cy3 or Cy5 Mono NHS Ester (CyDye postlabeling reactive dye pack; GE Health- care) and purified using Megaclear. Incorporation of Cy5 and Cy3 was measured using 1 μl of probe in a Nanodrop spectrophotometer ... set version 2.2 (QIAGEN, Hilden, Germany) were obtained from the Genomic and Microarrays Laboratory (Cincin- nati University, Cincinnati, OH). Information about print...

Ngày tải lên: 20/06/2014, 01:20

... quality of life (QOL) by maximising functions affected by the disease and its therapy. Preliminary research suggests that exercise may be an effective intervention for enhancing QOL in cancer ... preliminary evidence for the safety, feasibility, and efficacy of exercise training in breast cancer survivors. The aim of this study was to assess the association between physical exerci...

Ngày tải lên: 08/08/2014, 16:23