Báo cáo y học: "Caveolin-1 expression and stress-induced premature senescence in human intervertebral disc degeneration" docx
Báo cáo y học: "Caveolin-1 expression and stress-induced premature senescence in human intervertebral disc degeneration" docx
... articleCaveolin-1 expression and stress-induced premature senescence in human intervertebral disc degenerationSarah Kathleen Heathfield1, Christine Lyn Le Maitre2 and Judith Alison Hoyland11Tissue ... Hoyland JA, Freemont AJ: Interleukin-1 receptorantagonist delivered directly and by gene therapy inhibitsmatrix degradation in the intact degenerate human interverte-bral disc: an in situ zymographic ... glycoxidation product N(epsilon)-(carboxymethyl)lysine in human tissues in diabetes and aging. J Clin Invest 1997,99:457-468.37. Le Maitre CL, Freemont AJ, Hoyland JA: The role of interleukin-1in...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... cystatins, thekininogens, are larger proteins containing three tandemlyrepeated type 2-like cystatin domains. Type 2 cystatinsidentified in higher animals include cystatins C, D, S, SA, and SN [2], ... arereadily secreted in mammalian [20] and insect [5] cells,respectively. Cystatin F is an unusual type 2 cystatin in thatit is a glycoprotein [5,6], and it is known that many proteinsfound in intracellular ... N-glycosylationmotifs at positions 36–38 and 88–90 (cystatin C numbering).Recombinant cystatin F expressed in Sf9 insect cells isindeed glycosylated [5].Compared to other cystatins, cystatin...
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... reactions were incubated at 30 °Cfor90 min and the products analyzed on 4.5% nondenaturingPAGE. Following staining in Coomassie blue and destain-ing, the gels were soaked in Amplify solution (Amersham),dried ... symmetry and is composed of sevento nine distinct polypeptides (designated as a, b, c, etc.) [8]whereas GroEL has a sevenfold symmetry and is made of asingle polypeptide chain. In addition, and in ... respectively), and mapped on tubulin secondarystructure as determined by Incla`n and Nogales [47]. Arrows and rectangles indicate b-sheets and a-helices, respectively. The labels ÔLÕ and ÔMÕindicate...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... nitroalkane by NaoA.Keywords: enzymatic properties; expression; gene cloning;nitroalkane-oxidizing enzyme; Streptomyces.Nitroalkane compounds are widely used in chemical indus-try as intermediates, ... Electro-phoresis Power Supply and a Mini IEF Cell (Bio-Rad).Protein standards of different pI were as follows: amylo-glucosidase, pI 3.6; b-lactoglobulin A, pI 5.1; myoglobin, pI6.8/7.2; trypsinogen, pI 9.3. ... The pI of NaoA was determinedfrom a standard curve of pI and migration distance (cm) ofprotein standards.Enzyme assay and analytical methodsE. coli BL21(DE3) containing plasmid pNA101 was grownat...
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Báo cáo y học: " Tumour necrosis factor-α stimulates dehydroepiandrosterone metabolism in human fibroblast-like synoviocytes: a role for nuclear factor-κB and activator protein-1 in the regulation of expression of cytochrome p450 enzyme 7b" doc
... an increase in Cyp7bactivity and Cyp7b mRNA level in synovial biopsies [9].Recently, we reported that Cyp7b mRNA expression and Cyp7b activity are present in FLS from patients with RA [10]. In ... Cyp7b expression. Use of the MAPK inhibitors PD98059 and SB203580 did notresult in convincing changes in TNF-α-induced Cyp7b mRNA expression. We then determined Cyp7b enzymatic activity in FLS ... 7α-OH-DHEA. In addition, intra-articular delivery of 7α-OH-DHEA and/ or Cyp7b expression systems should add to our understanding of the role played byCyp7b in the arthritic process.The inhibitory effect...
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Báo cáo y học: "Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblast" potx
... MMPs,proinflammatory cytokines and chemokines [13].There is increasing evidence that SFs are key players in thepathogenesis of RA by invading and eroding hyaline cartilage.SFs, activated locally, ... growthfactor, insulin-like growth factor-binding proteins), morpho-gens, enzymes, numerous cytokines and chemokines, inter-leukins, and so forth [52]. It can be hypothesized that astringent control ... involved in mineral and vitamin Dmetabolism. Kidney Int 2007, 71:730-737.7. Nyirkos P, Golds EE: Human synovial cells secrete a 39 kDaprotein similar to a bovine mammary protein expressed duringthe...
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Báo cáo y học: "Ozone Therapy and Hyperbaric Oxygen Treatment in Lung Injury in Septic Rats"
... affected initially, and sepsis leads to severe injury in lung tissue [5]. It has been shown that pericytes in lung tissue produce proinflammatory cytokines in response to lipopolysaccharide ... and clinical studies in animal models and human beings are needed to un-derstand molecular elements, ligand and receptors, involved in the mode of action of ozone and HBO. Finally, according ... reduced oxidative stress indices and myeloperoxidase activity, levels of serum proinflammatory cytokines and histopathological injury scores considering lung tissue in septic rats. Compared...
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx
... aninvariantpartofallthreeclassesoftheplantperoxidasesuperfamily [44]. This motif (shown in grey shading in Fig. 1) includes Ser96 and Asp99 located at the beginningof helix D and connecting to the following long loop in atight hydrogen bonding network ... promoteractivity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2,responded strongly to wounding. The AtP38 gene mightrespond similarly.The Arabidopsis ... in tandem by AtPCb on chromosome 3, whereasAtP16 is single (gene numbers and names in Table 1;protein similarity in Fig. 1). The genes for AtP38 and At4g08780 are also in tandem, however, in...
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Báo cáo hóa học: " Subcellular forms and biochemical events triggered in human cells by HCV polyprotein expression from a viral vector" doc
... aliquot of Cy3 or Cy5 Mono NHSEster (CyDye postlabeling reactive dye pack; GE Health-care) and purified using Megaclear. Incorporation of Cy5 and Cy3 was measured using 1 μl of probe in a Nanodropspectrophotometer ... setversion 2.2 (QIAGEN, Hilden, Germany) were obtainedfrom the Genomic and Microarrays Laboratory (Cincin-nati University, Cincinnati, OH). Information aboutprinting and the oligonucleotide set can ... impact of HCV polyprotein expression in human cellshas been hampered by limitations of different cell systemsto express the entire HCV polyprotein in high yields and in all cells. Vaccinia virus...
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Báo cáo y học: "Physical Exercise and Quality of Life in Breast Cancer Survivors" potx
... quality of life (QOL) by maximising functions affected by the disease and its therapy. Preliminary research suggests that exercise may be an effective intervention for enhancing QOL in cancer ... preliminary evidence for the safety, feasibility, and efficacy of exercise training in breast cancer survivors. The aim of this study was to assess the association between physical exercise and ... oncology departments. Key words: Physical exercise, quality of life, breast cancer survivors, Italian Oncology INTRODUCTION Physical activity has many and varied effects on the human body. The...
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