... that RNAi-mediated silencing of EGFR mayprovide an opportunity to develop a new treatment strat-egy for NSCLC.Materials and methodsCell lines and cell culture A5 49 and SPC -A1 are well-characterized ... Pulmonary Diseases, Zhong Shan Hospital, Fudan University, Shanghai, PR China and 2Department of Medicine, University of Queensland, Prince Charles Hospital, Brisbane, AustraliaEmail: Min Zhang ... production of EGFR protein. Quantitative reverse-transcriptase PCR was used to detect the level of EGFR mRNA. Cell count, colony assay, scratch assay, MTT assay in vitro and tumor growth assay in athymicnude...