Báo cáo y học: "Activation of proteinase-activated receptor 2 in human osteoarthritic cartilage upregulates catabolic and proinflammatory pathways capable of inducing cartilage degradation: a basic science study" pot

... of data, statistical analysis, and manuscript preparation. NA participated in acquisition of data, analysis and interpretation of data, statistical analysis, and manuscript preparation. JMP and ... citation purposes) Vol 9 No 6 Research article Activation of proteinase-activated receptor 2 in human osteoarthritic cartilage upregulates catabolic and proin...

Ngày tải lên: 09/08/2014, 10:22

... hospitalizations each year. 1 Previously characterized as a disease of air- way smooth muscle, asthma is currently defined by the National Heart, Lung, and Blood Institute as a chronic inflammatory ... and adults, result- ing in increased physical activity and quality of life. Figure 2 Comparison of montelukast (ϫ) with sal- meterol (•) in change from baseline in maximu...

Ngày tải lên: 08/08/2014, 20:23

... JR and VK undertook some of the genotyping assays on DNA prepared in the laboratory of EAJ and RWO, who partic- ipated in the original design of the study. FP and MA gave invaluable advice during ... IIIB-NA1PR. The NA2 assay similarly included the IIIB-NA2F and IIIB-NA2R ARMS primers and internal control primers IIIB-NA2PR and IIIB-NA2PR from the gene MCSD1. Statistical...

Ngày tải lên: 09/08/2014, 07:20

... 8 325 -4 and its mutants lacking aureolysin, serine protease, and cysteine protease, demonstrated in a murine SA model that inactivation of the Figure 4 Potentiation of matrix metalloproteinase ... an inflammatory cytokine-mediated pathway in the observed induction of MMPs by S. aureus. S. aureus culture supernatants and cell lysates have a wide variety of proteins, and...

Ngày tải lên: 09/08/2014, 08:23

... CGGTATTTCACACCG CAcATGGTGCACT CTCAGTACAATC. The primer to mutate Q288 to alanine in the ligand-binding pocket was: GTGCCAAGTGGTCA ACAAA gcGCTCTTCCAGATGGTCGAATAC. A pri- mer that targeted two amino ... transcriptionally activate or antagonize an orphan receptor would aid the discovery of regulatory pathways in which the receptor participates. Finally, transcriptional agonists an...

Ngày tải lên: 23/03/2014, 21:20

... protein involved in the regulation of gamma secretase cleavage generating amyloid beta protein [3] and appears to play a crucial role in the normal metabolism of beta amyloid precursor protein as well ... the constructs are indicated below the graph. m1 is a mutation from CCGGAAATGACGA to CC ttAAATGACGA eliminating the )10 Ets site. In m6 the mutation to CCGGAAAT cgaGA elimina...

Ngày tải lên: 08/03/2014, 09:20

... observation that OMPLA variants having an impaired dimeric interface also lacked high a nity calcium binding. A covalent linkage was not able to restore high a nity calcium binding in these variants, ... noteworthy that all mutations at the dimer interface are located at more than 15 A ˚ from the catalytic calcium ion (Fig. 1B). In the standard assay, wild- type OMPLA has a calciu...

Ngày tải lên: 24/03/2014, 03:21

... suppressors of cytokine signaling (SOCS-1 and 3). The SOCS proteins act as negative regulators of the JAK/STAT1 pathway either by binding and inhibiting JAK tyrosine kinases or by inhibiting binding of ... phospho-specific Tyr1 022 /1 023 JAK1 or Tyr1007/1008 JAK2 antibody (Cell Signaling Technology, Beverly, MA) and then stripped and finally analyzed by total JAK1 or JAK2 anti...

Ngày tải lên: 19/06/2014, 22:20

... Freeman GJ: The B7-CD28 superfamily. Nat Rev Immunol 20 02, 2: 116- 126 . 20 . Matsuyama T, Yamada A, Kay J, Yamada KM, Akiyama SK, Schlossman SF, Morimoto C: Activation of CD4 cells by fibronectin and ... 12- myristate 13-acetate (PMA) and ionomycin for 24 h or frozen directly in TriReagent for RNA isolation and real-time polymerase chain reaction, as described in Materials...

Ngày tải lên: 09/08/2014, 01:23

... joint and recorded in proximal interphalangeal joints, metacarpophalangeal joints, carpal joints, wrist joints and met- atarsophalangeal joints. Thirty-nine patients out of 62 had ero- sive disease. ... that soluble RAGE may block the ligand–RAGE interaction on the cell surface by directly bind- ing leukocyte 2- integrin Mac-1 and thereby decreasing influx of inflammatory cells i...

Ngày tải lên: 09/08/2014, 06:23