... a1-antitrypsin, a1-antichymotrypsin, and anti-thrombin III resulted in an increased conformationalstability and a decreased specific inhibitory activity[23,24]. Lys335, localized in b strand 5A, ... chains of theamino acids in positions 53 and 56 in a helix B, 186 in bstrand 3A, and position 334 in b strand 5A (Fig. 1; thenumbering of amino acids in PAI-1 is according to thea1-antitrypsin ... pole of the serpin [7– 10]. Thetranslocation results in distortion of the proteinase [11] and inactivation of the enzymatic machinery [10]. Delayed RCLinsertion results in hydrolysis of the...