... a single set of identical independent bind-ing sites, using the Bindwork software supplied with the instrument, which provided the stoichiometry of binding (nligand : protein), the change in ... function of the molar ratio, indicate a binding stoichiometry of 1 : 1 for ERB-hRNase and Erbicin and of 0.5 : 1 for ERB-hcAb and Herceptin, i.e. each identical antigenbinding site binds one ... immunoagents in NaCl ⁄ Pisolu-tion were injected into the ErbB2-ECD solution in NaCl ⁄ Piat 400 s intervals. Binding curves involved the addition of 25 injections. The heat of dilution of the immunoagentsinto...