Báo cáo y học: "Human meniscus cells express hypoxia inducible factor-1α and increased SOX9 in response to low oxygen tension in cell aggregate culture" ppsx

... article Human meniscus cells express hypoxia inducible factor-1α and increased SOX9 in response to low oxygen tension in cell aggregate culture Adetola B Adesida 1,2 , Lisa M Grady 1 , Wasim ... determine whether hypoxia inducible factor (HIF)-1α and downstream target genes that are involved in the adaptive response of cells and tissues to...

Ngày tải lên: 09/08/2014, 10:20

... α-chymotrypsin in 50 mmol/l Tris-HCl (pH 7.6; with the following proteinase inhibitors: 1 mmol/l EDTA, 1 mmol/l iodoacetamide, and 10 μg/ml pepstatin A). After the α-chymotrypsin activity was inhibited ... to inhibit protein kinase C activ- ity and translocation [20,49], and protein kinase C was shown to contribute to MMP-1 and MMP-13 expression [50,51]. 15-HETE and 13-HODE...

Ngày tải lên: 09/08/2014, 14:20

... performed using BrdU and CFSE staining. Flow cytometry was performed to determine Foxp3 expression, cell proliferation, viabilities and phenotype analyses of T cells. Results: Both CD4+ and CD8+ T cells ... CD4+ and CD8+ T cells on pooled samples were determined on days 0, 3, and 6 post-culture by flow cytometry analysis (B). Expression of FoxP3 and CD25 were determined...

Ngày tải lên: 18/06/2014, 15:20

... antibody in the sec- ond cycle. Astrocytes were detected by GFAP staining with a Texas Red tagged secondary antibody (Fig. 3G and 3J) in the first cycle and CD59 staining (Fig 3H) or C1-inh stain- ing ... clusterin [22]. Astro- cytes [23], neurons and Schwann cells have been reported to express CD59 [24] and neuroblastoma cell lines C1-inh [18]. Astrocytoma cell lines ha...

Ngày tải lên: 19/06/2014, 22:20

... induce the expression of cytokines (such as IL-6, granulocyte/macrophage colony- stimulatory factor and granulocyte colony-stimulatory factor) and chemokines (such as IL-8, CXC chemokine ligand ... with systemic lupus erythematosus [72] or systemic sclerosis [73]. Recently, increased expression of CC chemokine ligand 20 (the chemokine able to bind CC chemokine receptor 6 expressing Th...

Ngày tải lên: 09/08/2014, 10:23

... radial and circumferential strain and strain rate by 2DST in normo-, hypo-, akinetic and dyskinetic seg- ments of post-infarct patients 8-13, 30-37 , which were not reliably accessible by TDI. ... Chen J, Cao T, Duan Y, Yuan L, Yang Y. Velocity vector imag- ing in assessing the regional systolic function of patients with post myocardial infarction. Echocardiography 2007; 24(9...

Ngày tải lên: 25/10/2012, 11:15

... surface staining showed the IPFP cell population to be fairly homogeneous and to express a group of epitopes commonly found on other adult stem cells. Clonally expanded infrapatellar fat pad cells Freshly ... neural, or myogenic progenitors or stem cells. Evidence for pericytes in the IPFP tissue and IPFP- derived cells 3G5 distinctively stains pericytes and these cel...

Ngày tải lên: 09/08/2014, 10:23

... currently analyzing the levels of pro-inflammatory cytokine in T-MPC culture medium. In support of our theory that expo- sure to pro-inflammatory cytokines suppressed the differentia- tion capacity ... proliferation (both for naive and memory T cells) , cytotoxic T-lymphocyte formation, IFN-γ production by Th1 cells, and interleukin-4 pro- duction by Th2 cells. [39,40]. The abili...

Ngày tải lên: 09/08/2014, 10:23

... cell types that include neurons, hematopoietic cells, cardiomyocytes, and insulin-secreting cells, to name a few [3-9]. Many new studies are currently directed towards expanding the use of hES cells ... analysis of differentiating DCs from hES and FL CD34+ cellsFigure 2 FACS analysis of differentiating DCs from hES and FL CD34+ cells: CD34+ cells were cultured in cytokine m...

Ngày tải lên: 10/08/2014, 05:20

... RNA-activated protein kinase. Results: In immunohistochemical studies, TLR3 mainly expressed in the cytoplasm of ganglion cells and in some neuroblastic cells, but not in the stromal cells in human NB ... TLR3 agonist, polyinosinic-polycytidylic acid (poly(I:C)), on the growth of human NB cells was evaluated by WST-1 cell proliferation assay, flow cytometry analysis, and...

Ngày tải lên: 10/08/2014, 10:20