Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx

... (f) An area of an OA synovium demonstrates a lining layer completely devoid of c19orf10 staining. (g) Intense staining of a hyperplastic RA synovial lin- ing cell layer. This staining was typical ... Expression of c19orf10 in OA synovium. (a) Intense staining of the synovial lining layer and perivascular regions of RA (OCT sec- tion) tissue.(b) Intense staining of t...

Ngày tải lên: 09/08/2014, 10:20

... 10 )5 M forskolin, an adenylate cyclase activator. A pattern similar to the thyrotropin stimulation was observed in these experimental conditions. Activation of the tyrosine kinase/MAP kinase pathway by EGF ... intermediate filaments, and with the appearance of a marked cytokeratin and actin immunoreactivity at the cell junctions [28,42]. The latter cytoskeleton changes...

Ngày tải lên: 23/03/2014, 21:20

... resulting supernatant was used for purification of the recombinant protein. Purification of the recombinant phosphohistidine phosphatase A 9-mL sample of the supernatant of the bacterial lysate was ... Institute of Cancer Research, Uppsala. The O-phosphopeptides GRRPsLFG and APDyTTPEMY-NH 2 were obtained from Dr A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analy...

Ngày tải lên: 21/02/2014, 01:21

... identity and 51.2% similarity) and N-acyl- D -asparate amidohydrolase (48.5% identity and 56.5% similarity) from Alicaligenes xylosoxydans ssp. xylosoxydans A- 6 and the D -aminoacylase from Alicaligenes ... specificity analysis To study the substrate specificity of the N-D-AAase protein, the activity of the enzyme against N-acyl- D -or L -amino acids and other D -ami...

Ngày tải lên: 08/03/2014, 16:20

... of NaoA was determined from a standard curve of pI and migration distance (cm) of protein standards. Enzyme assay and analytical methods E. coli BL21(DE3) containing plasmid pNA101 was grown at ... recombinant NaoA after DEAE-Sepharose Fast Flow chromatography; lane 6, standard molecular mass markers (phosphorylase b,97kDa;BSA,66kDa; ovalbumin, 45 kDa). Fig. 3. Assay of NaoA act...

Ngày tải lên: 31/03/2014, 08:20

... CaLP and CaM appeared as a single band with an apparent molecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence of cal- cium, the apparent molecular mass was ... by a slight blue shift of the peak in 209 nm. The calcium binding and conformational changes of oyster CaLP and CaM were further investigated by monitoring intrinsic p...

Ngày tải lên: 16/03/2014, 23:20

... temperature in SOC medium and then plated on Luria–Bertani agar plates containing kanamycin. Kanamycin resistant (Km R )trans- formants were selected and colony-purified on agar plates incubated ... The ability of the mutant strain to resume growth after the stationary phase was then compared with that of the parental strain. As shown in Fig. 6A, after transfer from station...

Ngày tải lên: 22/02/2014, 07:20

... that the main contamination, seen in lane 1, run s only m arginally above the hA2aR fusion pro tein and is the main band in lane 2. In this pu ri®cation, 7% of speci®c ligand binding sites loaded ... hA2aR fusion protein in quantity and quality suf®cient for biophysical characteriza- tion and crystallization. The f ollowing points m ade the puri®cation of large amo...

Ngày tải lên: 24/03/2014, 00:21

... enzyme Liver enzyme SNA (Sambucus nigra A. ) – + + GNA (Galanthus nivalis A. ) + – – MAA (Maackia amurensis A. ) – – – DSA (Datura stramonium A. ) – – – ConA (Concanavalin A) + + + WGA (Wheat germ A. ) ... m M DFP and 1 m M 2-iodoacet- amide for p ost proline cleaving enzyme and pyroglutamate aminopeptidase, respectively). As a measure for the enzy- matic activity, the initial...

Ngày tải lên: 31/03/2014, 21:21

... with glycoproteins and sugars were helpful in deriving the binding affinity of the humoral agglutinin. BSM contains the sialic acids, N-acetylneuraminic acid, N-glycolylneu- raminic acid, N-acetyl 9-O-acetylneuraminic ... sialic acid affinity of the Paratelphusa lectin was further proved by its inability to inhibit sialidase-treated BSM and bovine thyroglobulin. Also the lec...

Ngày tải lên: 21/02/2014, 00:20