Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
... (f) An area of an OA synovium demonstrates a lining layer completely devoid of c19orf10 staining. (g) Intense staining of a hyperplastic RA synovial lin-ing cell layer. This staining was typical ... Expression of c19orf10 in OA synovium. (a) Intense staining of the synovial lining layer and perivascular regions of RA (OCT sec-tion) tissue.(b) Intense staining of the synovial lining layer and ... minimal staining of the lymphocytes although some mononuclear cells in the aggregates stained positively (arrow). (e) Intense staining of individual cells in the lining layer of a typical OA synovium....
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... 10)5Mforskolin,an adenylate cyclase activator. A pattern similar to the thyrotropin stimulation was observed in these experimentalconditions.Activation of the tyrosine kinase/MAP kinase pathwayby EGF ... intermediatefilaments, and with the appearance of a marked cytokeratin and actin immunoreactivity at the cell junctions [28,42]. The latter cytoskeleton changes in culture may be related to the in vivo secretory ... moresustained increase was observed, suggesting that newlysynthesized protein(s) may be involved in the destabilization of the mRNA. Thus p76RBEbehaves as an immediate earlygene of the cyclic AMP...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... resulting supernatant was used for purification of the recombinant protein.Purification of the recombinant phosphohistidinephosphatase A 9-mL sample of the supernatant of the bacterial lysatewas ... Institute of CancerResearch, Uppsala. The O-phosphopeptides GRRPsLFG and APDyTTPEMY-NH2were obtained from Dr A ˚keEngstro¨m at the Peptide Synthesis and Analysis Laboratory of the Department of ... methanol at 1 mLÆmin)1. The absorbanceat 315 nm was monitored and the degree of dephosphory-lation was calculated from the peak areas of phosphorylated and dephosphorylated peptides. In a control...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... identity and 51.2% similarity) and N-acyl-D-asparate amidohydrolase(48.5% identity and 56.5% similarity) from Alicaligenesxylosoxydans ssp. xylosoxydans A- 6 and the D-aminoacylasefrom Alicaligenes ... specificity analysisTo study the substrate specificity of the N-D-AAase protein, the activity of the enzyme against N-acyl-D-orL-amino acids and otherD-amino acid derivates was determined (Table ... process of N-D-AAase and N-acylamino acid racemase. However,some N-D-AAases isolated from bacteria have someL-aminoacylase activity [18,33]. Therefore, it is necessaryto avoidL-aminoacylase interference...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... of NaoA was determinedfrom a standard curve of pI and migration distance (cm) of protein standards.Enzyme assay and analytical methodsE. coli BL21(DE3) containing plasmid pNA101 was grownat ... recombinant NaoAafter DEAE-Sepharose Fast Flow chromatography; lane 6, standardmolecular mass markers (phosphorylase b,97kDa;BSA,66kDa;ovalbumin, 45 kDa).Fig. 3. Assay of NaoA activity. (A) ... identify whether the protein encoded by the completeDNA fragment can catalyze the oxidation of nitroalkanes. In this paper, we describe the cloning and characterization of a novel gene (naoA) that encodes...
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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
... CaLP and CaM appeared as a single band with an apparentmolecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence of cal-cium, the apparent molecular mass was ... by a slightblue shift of the peak in 209 nm. The calcium binding and conformational changes of oyster CaLP and CaM were further investigated bymonitoring intrinsic phenylalanine and tyrosine ... reactions. The first round of PCRreaction was performed with a forward primer UPM (a mixture of primers 5¢-CTAATACGACTCACTATAGGGCAAGCAGTGGTAACAACGCAGAGT-3¢ and 5¢-CTAATACGACTCACTATAGGGC-3¢) and...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... temperature in SOCmedium and then plated on Luria–Bertani agar platescontaining kanamycin. Kanamycin resistant (KmR)trans-formants were selected and colony-purified on agar platesincubated ... The ability of the mutant strain toresume growth after the stationary phase was then comparedwith that of the parental strain. As shown in Fig. 6A, aftertransfer from stationary cultures into ... 142–144 amino-acids long; they are acidic and presumably located in the cytoplasm like UspA.Members of this family share a strikingly similar hydro-pathy profile (data not shown), and UP12 shares...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... that the main contamination,seen in lane 1, run s only m arginally above the hA2aR fusion pro tein and is the main band in lane 2. In this pu ri®cation, 7% of speci®cligand binding sites loaded ... hA2aR fusion protein in quantity and quality suf®cient for biophysical characteriza-tion and crystallization. The f ollowing points m ade the puri®cation of large amounts of functional hA2aR ... ligand, a half-life of 33 dayswas obtained. This increased to 77 days in the presence of NECA (agonist) and 53 days in the presence of theophylline(antagonist) (one experiment, eight time points...
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Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc
... enzyme Liver enzymeSNA (Sambucus nigra A. ) – + +GNA (Galanthus nivalis A. ) + – –MAA (Maackia amurensis A. ) – – –DSA (Datura stramonium A. ) – – –ConA (Concanavalin A) + + +WGA (Wheat germ A. ) ... mMDFP and 1 mM2-iodoacet-amide for p ost proline cleaving enzyme and pyroglutamateaminopeptidase, respectively). As a measure for the enzy-matic activity, the initial rate of TRH-degradation ... p lasma of neonatal rats, whereas TRH is rapidly inactivated byplasma of adult rats [39]. The endocrinological importance of this enzyme was subsequently questioned by the findingsthat the activity...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... withglycoproteins and sugars were helpful in deriving the binding affinity of the humoral agglutinin. BSM contains the sialic acids, N-acetylneuraminic acid, N-glycolylneu-raminic acid, N-acetyl 9-O-acetylneuraminic ... sialic acid affinity of the Paratelphusa lectin wasfurther proved by its inability to inhibit sialidase-treatedBSM and bovine thyroglobulin. Also the lectin failed toagglutinate desialylated rabbit ... for preparing asialo-erythrocytes was that of Mercy and Ravindranath [24].Sialidase treatment of sialoglycoprotein. Asialo-glycopro-teins were prepared by incubating 2 mg of glycoprotein with0.1...
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