Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study ppsx

... to 28 days (chronic phase of inflammation) after induction of AIA. The patella and the menisci of the joints with adjacent synovial tissue were separated and cultured in 24-well plates in DMEM (Gibco, ... 1:153-171. 23. Dray A, Perkins M: Bradykinin and inflammatory pain. Trends Neurosci 1993, 16:99-104. 24. Heppelmann B, Pawlak M: Sensitisation of articular affer...

Ngày tải lên: 09/08/2014, 10:20

... GTTTGAATTGGCCAGAGGAA reverse, TCTGTTGGAAAATCCCGTTC Cxcl10 forward, CCCACGTGTTGAGATCATTG reverse, GAGGAACAGCAGAGAGCCTC Cxcl10 pre-mRNA forward, AGCAGAGGAAAATGCACCAG reverse, CACCTGGGTAAAGGGGAGTGA Dusp16 ... CCTAGCTTGGCTGACAGAGG; reverse, CTGCTCCTTCTCCTTCATGC Asns forward, TACAACCACAAGGCGCTACA; reverse, AAGGGCCTGACTCCATAGGT Trb3 forward, CAGGAAGAACCGTTGGAGTT; reverse, TTGCTCTCGTTCCAAAAGGA Actb...

Ngày tải lên: 07/03/2014, 03:20

... (5¢-AG CTGCCTGCAGGTCGACTCTAGAGGATCCTAACCAA TTTTGCTGCC-3¢); for OR17-40 (5¢-CGTCAAGGAG AAAAAACCCCGGATCTAAAAAATGGAGCAGAAAC TCATCTCTGAAGAGGATCTG-3¢) and (5¢-GCATG CCTGCAGGTCGACTCTAGAGGATCTCAAGCCAGT GACCGCCTCCC-3¢); ... was performed on DNAse- treated RNA extracts. Primers used for RT-PCR were: for the I7 OR (5¢-CGTCAAGGAGAAAAAACCCCGGATCT AAAAAATGGAGCGAAGGAACCACAG-3¢) and (5¢-AG CTGCCTGCAGG...

Ngày tải lên: 07/03/2014, 16:20

... substantially in Afghanistan and Guatemala and the application of active management of third stage of labor (AMTSL) in several countries including Niger, Mali, Afghanistan, and Ecuador increased ... substantially.  Essential Newborn Care: In Uganda, the ability of the health facility staff to detect neonatal asphyxia and immediately apply resuscitation increas...

Ngày tải lên: 23/03/2014, 06:20

... in circulating leukocytes. The data suggest that PPARα activators may be useful in the attempt to decrease the risk of atherosclerosis and may act as modulators of systemic inflammation and the ... manuscript. JJ, JC and JMA con- tributed to the final version of the manuscript. GA, RB-D and AR helped in the data analysis and interpretation. JJ and JC supe...

Ngày tải lên: 10/08/2014, 05:21

... intracellular flow cytometric analysis of signaling pathways, such as the NF-κB and MAPK cascades, can be used routinely to assess the activation status of a small number of cells and thus delineate abnormalities ... the number of samples is great, such as in a clinical trial. Advantages and disadvantages of multiparameter intracellular flow cytometric analysis...

Ngày tải lên: 09/08/2014, 01:23

... and careful discus- sion of the data. CB coordinated the study, was involved in the critical discussion of results and their interpretation and helped to draft the article. All authors read and ... the synovial membrane and the formation of a pannus, which leads to swollen joints and finally to joint destruction. Inflammatory cells such as monocytes a...

Ngày tải lên: 09/08/2014, 06:23

... Henan Provincial Institute of Cancer, Zhengzhou, Henan Province, China. 2 Department of Pathology, Henan Provincial Cancer Hospital, Henan Provincial Institute of Cancer, Zhengzhou, Henan Province, ... RK, Williams NS, et al: Adjuvant chemotherapy versus observation in patients with colorectal cancer: a randomised study. Lancet 2007, 370:2020-9. 3. Shanmugam Chandrakumar, Hines Rob...

Ngày tải lên: 10/08/2014, 21:23

... chromatin-bound proteins (Fig. 3 and Table 2). In contrast, the amounts of total Fig. 4. Abundance of Mcm2 mRNA in HeLa and WI-38 cells. (A) Total mRNA was purified from HeLa and WI-38 cells, and the ... fragment. These fragments are indicated. (B) Total RNA was purified from HeLa and WI-38 cells and analyzed by Northern blot analysis. Increasing volumes (0.7, 1....

Ngày tải lên: 20/02/2014, 23:20

... would have to take into account the evolution of real wages as well as of wage arrears. However, a remarkable point is that a substantial proportion of firms (31.5% of the sub-sample) has increased ... variables -the coordinates of each firm along axis f1 and f2- which are continuous and orthogonal, by construction, and incorporate a large fraction of the inform...

Ngày tải lên: 06/03/2014, 08:20