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Báo cáo khoa học: " Systematisation of spatial uncertainties for comparison between a MR and a CT-based radiotherapy workflow for prostate treatments" pptx

Báo cáo khoa học:

Báo cáo khoa học: " Systematisation of spatial uncertainties for comparison between a MR and a CT-based radiotherapy workflow for prostate treatments" pptx

... AccessResearch Systematisation of spatial uncertainties for comparison between a MR and a CT-based radiotherapy workflow for prostate treatmentsTufve Nyholm*1, Morgan Nyberg2, Magnus G Karlsson3 and Mikael Karlsson4Address: ... we compared the spatial uncertainties associated with a MR- based workflow for external radiotherapy of prostate cancer to a standard CT-based workflow. The MR- based workflow relies on target ... KR, Takai Y, Mitsuya M, Nemoto K, Ogawa Y, Yamada S:Evaluation of inter- and intrafraction organ motion duringintensity modulated radiation therapy (IMRT) for localized prostate cancer measured...
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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... fragmentation pattern. For quantification of SE, lipid extracts were applied toSilica Gel 60 plates with the aid of a sample applicator (Auto-matic TLC Sampler 4; CAMAG, Muttenz, Switzerland) and chromatograms ... in membranes butalso the amount of sterols derived from SE that areTable 1. Sterol analysis of cells lacking STE20 and CLA4. Data are mean values with standard deviation from at least two independentexperiments.lg ... indicated strainswere grown to stationary phase and then lipids were extracted and separated by TLC. The amount of SE of the wildtype strain was setat 100%. Data are mean values of three independent...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... were purchasedfrom Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade.AcknowledgementsThanks are due to Prof G.L. Sottocasa and Dr Anto-nella Bandiera (University ... legitimate to apply theScrutton and Utter equation [40] to the inhibition data.Data analysesData were analysed by means of sigmaplot 2001 (SPSSScience Software Gmbh, Erkrath, Germany). Data for ... simple anatomical structure,with a single layer of epidermal cells, featured by a large vacuole containing anthocyanins. On the otherhand, carnation petals have already provided a suit-able material...
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Tài liệu Báo cáo khoa học: Control of the coagulation system by serpins Getting by with a little help from glycosaminoglycans pptx

Tài liệu Báo cáo khoa học: Control of the coagulation system by serpins Getting by with a little help from glycosaminoglycans pptx

... concentration of AT and therates of interaction in the presence and absence of hep-arin pentasaccharide (Table 1), one can calculate thatthe half-life of enzyme activity in the absence of hep-arin ... of serine proteases of the coagulation system including thrombin (factorIIa) and factors IXa, Xa, XIa and XIIa. The princi-pal targets of the serpin are usually regarded as beingthrombin and ... [42,45,49,50,53].Instead, the major mechanism of GAG enhancementappears to be allosteric and uses conformational acti-vation of the serpin. Heparin cofactor II possesses a unique amino-terminal extension that...
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Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx

Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx

... repression of transactivation activity and self-association of GRIP1 (Figs 1–5), we examined therelationship between transactivation and co-activation of GRIP1, using a series of C-truncations ... for its co-activationin the AR system, AD1 activity is necessary for its co-activation in the TR system, and cross-talk between AD 1and AD2 activities is necessary for maximal co-activation in ... two autonomous activationdomains (AD1 and AD2) in the C-terminal region. AD1 activity appearsto be mediated by CBP ⁄ p300, whereas AD2 activity is apparently mediatedthrough co-activator-associated...
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Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

... bp [51];human b-actin, 5¢-ACAATGAGCTGCTGGTGGCT-3¢ and 5¢-GATGGGCACAGTGTGGGTGA-3¢; murine b-actin,5¢-TGGAATCCTGTGGCATCCATGAAAC-3¢ and 5¢-TAAAACGCAGCTCAGTAACCGTCCG-3¢. Human GAPDHprimers were ... Clontech, Mountain View,CA, USA), and 20 lL was used for PCR amplification usingthe following primers: human and murine ArgII, 5¢-GATCTGCTGATTGGCAAGAGACAA-3¢ and 5¢-CTAAATTCTCACACGTGCTTGATT-3¢ [50], ... Supernatants were analyzed for VSV titer using a standard plaque assay. Plaques were counted and titers calculatedas pfuÆmL)1. Values are representative of two experiments and areexpressed as...
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Báo cáo khoa học: Phosphorylation of NF-jB proteins by cyclic GMP-dependent kinase A noncanonical pathway to NF-jB activation potx

Báo cáo khoa học: Phosphorylation of NF-jB proteins by cyclic GMP-dependent kinase A noncanonical pathway to NF-jB activation potx

... Repository)was Flag-tagged at the 5¢-end by PCR using the pri-mers 5¢-CTGCAGCATGGACTACAAGGACGACGATGACAAGGAGAGTTGCTACAACCCAGGTCTG-3¢ and 5¢-GAGAGTTGCTACAACCCAGGTCTG-3¢with pRSV-p49 as a template. The amplified ... 5¢-AAACGTAAAAGGGCATATGAGACCTTCAAGAGCATC-3¢ for T30 5A (mutations in bold). The accuracy of the mutations wasconfirmed by DNA sequencing.p49 (obtained from the NIH AIDS Reagent Repository)was ... promoter,mediated most notably through SRE, FAP, and CRE [42].TheconnectionofPKGandNF-jB may have broadimplications, as a pathway that involves phospholipase A 2,hydroxyl radical, guanylate cyclase, and...
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Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc

Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc

... amounts of mRNA (Molecular Dynamics, CA,USA). The mRNA levels were analyzed in duplicatesamples and normalized to equivalent values for GAPDHto compensate for variations in loading and transfer.Messenger ... time periods and the amount of GTP-loaded RhoA (active form of RhoA) was deter-mined by pull-down assay as described in Materials and methods.Total amount of RhoA in the same samples was determined ... onRhoA activation was sustained for up to 15 min and did notalter the cellular levels of total RhoA. We also analyzed p38 and JNK phosphorylation status by Western blot and immunodetection analysis...
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Báo cáo khoa học: Inhibition of the NF-jB transcriptional activity by protein kinase A pot

Báo cáo khoa học: Inhibition of the NF-jB transcriptional activity by protein kinase A pot

... activation of PKA appear tomodulate the transcriptional activity of NF-jBinanegative way.In this report, we examined whether cAMP and PKAmodulates NF-jB activity and found that the cAMP/PKAsignaling ... Health, Labor and Welfare, the Ministry of Education, Culture, Sports, Science and Technology, and the Japanese Health Sciences Foundation. We thankDrs Shunsuke Ishii, Masatoshi Hagiwara, and David ... Takahashi, Toshifumi Tetsuka, Hiroaki Uranishi and Takashi OkamotoDepartment of Molecular Genetics, Nagoya City University Medical School, JapanThe cAMP-dependent protein kinase (PKA) signalingpathway...
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Báo cáo khoa học: Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism doc

Báo cáo khoa học: Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism doc

... thermal stability of the cis form between Azo and DM-azo. (A) Illustration of the reversible photoisomeriza-tion of Azo and thermal isomerization of cis-Azo. (B) Quantitativecalculation of cis-form ... involving one Azo and one DM-azo wasmaintained at 90 °C for 1 h after trans fi cis isomerizationas described above. Then, the template strand was added and annealed at 37 °C for 30 min. Thereafter, ... of the transcription reaction (ATPincorporation rate) for (A) trans–trans and (B) cis–cis forms by T7RNAP as a function of promoter concentration. The concentration of RNAP was maintained at...
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