... mammalian cells hadan N-terminal 3XFLAG tag and a C-terminal Myc tag. USP7-FL con-structs used for proteomics analysis contained either N- or C-termi-nal CBP-Protein A tags separated by a ... sera werepurchased from Sigma. Rabbit anti-mouse-HRP and goatanti-rabbit-HRP secondary sera conjugates were fromSigma and Biorad (Hercules, CA, USA), respectively. Goatanti-rabbit-Texas red ... BrendanKerins, Magali Perret, Sonia Buri (all from Novartis,Basel, Switzerland) and Markus Schirle, ManfredRaida, Anne-Marie Michon and Sonja Ghidelli (Cell-zome AG, Heidelberg, Germany) for...