Báo cáo khoa học: " The radiosensitizer 2-benzoyl-3-phenyl-6,7-dichloroquinoxaline 1,4-dioxide induces DNA damage in EMT-6 mammary carcinoma cells" potx

... stages in the mitotic phase. Immunofluor- escent staining with alpha-tubulin was used to identify the cells located at which stage during the mitotic phase. In Figure 4B, the majority of the mitotic ... CycleTEST plus DNA reagent kit (Becton Dickinson, Lincoln Park, NJ, USA) in the dark. Analysis of DNA histogram was performed on a FACScaliber flow cytometer (Becton Dick...

Ngày tải lên: 09/08/2014, 09:20

... accu- mulation induced by IR appear together in combination treatments. Despite our intriguing findings that the com- bination treatment DCQ+IR induces DNA damage, including DSBs, and slows repair, the ... Topoisomerase II inhibitor mitoxantrone [17]. Another major kinase activated by DNA damage is DNA- PK, which is activated by binding to the damaged sites on DNA [18]....

Ngày tải lên: 09/08/2014, 10:20

... he binding of the a-carboxylate group of the s ubstrate, when the external aldimine is formed. The anchoring of a-carboxylate and a-amino group in the external aldimine defines automatically the ... quinonoid intermediate structure [18], but not in the external aldimine. The observed predominant formation of the quinonoid intermediate in the reaction of TPL with L -me...

Ngày tải lên: 19/02/2014, 16:20

... are located in the cores of the large and small domains, respectively, and appear to stabilize their protein scaf- folds, allowing the proper positioning of the residues involved in PLP binding, substrate ... at the value determined from the amino acid sequence (45 320 Da). Measurement of the K d of PLP binding equilibrium PLP binding equilibria were analyzed taking advantag...

Ngày tải lên: 07/03/2014, 03:20

... observe a corresponding shift in the macroscopic ionization. Deuteration of the atoms bonded to the amine nitrogen is known to cause an increase in the amine pK a ; in part due to: (a) the shorter C-D ... steady-state kinetic studies on the pH dependence of monoamine oxidase led to the sug- gestion that it is the protonated form of the amine substrate that binds to th...

Ngày tải lên: 07/03/2014, 06:20

... tubulin in the presence of microtubule-associated proteins. The binding of sanguinarine to tubulin induces conformational changes in tubulin. Together, the results suggest that sanguinarine inhibits ... polymer-bound sanguinarine by sedimenting the polymers. The incor- poration of sanguinarine per tubulin dimer into the polymer increased with increasing concentration of sang...

Ngày tải lên: 07/03/2014, 12:20

... flexibil- ity in the A-chain resulting in fewer A–B interchain electrostatic ⁄ H-bonding contacts in the DK9 mutant. These A-chain folding effects should be allosterically transmitted to the active ... structures of human thrombin show that the A-chain closely follows the contour of the catalytic B-chain, hinging the two interacting six-stranded bar- rel-like domains of th...

Ngày tải lên: 07/03/2014, 12:20

... [10,12]. Nevertheless, it is not possible to assume that the same is occurring in the mutant R97M, because there is no data on the interactions between the substrate and the arginine residue. Finally, another ... m M Bicine buffer 7.0–8.5). The pH stability of Sfbgly50 was checked by incubation in the same buffers for a time equal to the assay time and then determining th...

Ngày tải lên: 23/03/2014, 15:21

... the interaction of the intrinsic strength of a boundary with the strengths of other boundaries in the sentence, as well as the distance between these boundaries. The most salient of the interactions ... material in the comptemennzer Wltla the material in the core sentence as they are analy.zing the material in the core bentence or after that analysis IS...

Ngày tải lên: 24/03/2014, 02:20

... after 3 min and then declined, while the formation of [ 3 H]ATP was increased linearly with time within the first 15 min. After 30 min, the reaction reached equilibrium (Fig. 4A). Further experiments ... resulted also in a high level of incorporation into DNA, but the level of labelled dTTP was very low [18]. In organisms where NDK is present, the rate-limit- ing step in the...

Ngày tải lên: 30/03/2014, 08:20