... G-protein-coupled receptor; and this binding activates thedownstream signaling pathways including the Gqheterotrimeric G protein, and then further increasesthe intracellular calcium level and ... with an MEK inhibi-tor, PD98059; a PLC inhibitor, U73122; a PKCinhibitor, bisindolylmaleimide I; and a general COXinhibitor, indomethacin, or a selective COX-2 inhibi-tor, NS-398, in the presence ... obtained from the Human ScienceResearch Resources Bank (Osaka, Japan), cultured in DMEM (Sigma, St Louis, MO, USA) containing 10%(v ⁄ v) fetal bovine serum and antibiotics, and maintained in a...