Báo cáo khoa học: "In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs" pdf

... RESEARCH Open Access In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs Takamitsu A Kato 1,2,3 , ... A):A1-A13. doi:10.1186/1748-717X-6-116 Cite this article as: Kato et al.: In vitro characterization of cells derived from chordoma cell line U-CH1...

Ngày tải lên: 09/08/2014, 09:21

... treated with 50 l M o-phenanthroline; P, untreated PTOX-expressing; PO, expres- sing PTOX and treated with o-phenanthroline; POF, expressing PTOX and treated with both o-phenanthroline and 125 ... in E. coli and studied its substrate preferences, the factors influencing its activity and the nature of suitable metal cofactors and inhibitors in vitro. Materials and method...

Ngày tải lên: 31/03/2014, 07:20

... 4. CD spectra and differential spectra of DDI-1A and DDI-1B and their complexes with selected DNA sequences. Solid line, 20 l M free DNA. Dashed line: (A,C,E,G,I) complex of DNA with DDI-1A (50 ... than those of their complementary strands, ATT and GT (Fig. 1B,C). As such, the enhancement effects of AB CDE F Fig. 1. Expansion of the various repeats and the effect o...

Ngày tải lên: 07/03/2014, 06:20

... pre-RNA derived from a PvuII-linearized pCOBsal DNA template was 632 nucleotides (nt) in length and contained 311 nt of intron sequence, 112 nt of 5¢ exon and 209 nt of 3¢ exon sequence. HindIII -derived ... demonstrate the specificity of endonuclease inhibition by prebound AnCOB RNA. The assay of 5A was repeated with and without 5 n M AnCOBDP9 pre-RNA and with KCl a...

Ngày tải lên: 17/03/2014, 10:20

Ngày tải lên: 07/08/2014, 18:21

... mM citrulline, 2.5 mM sodium ascorbate and 0.1% polyvinyl pyrrolidone 40 000) to avoid the browning of explants, and finally put into culture medium. Some aspects of in vitro ... superiority and then be distributed afterwards as selected clones. In vitro micropropagation is applied because of the poor development of cuttings. Materials and...

Ngày tải lên: 09/08/2014, 02:21

... FLAG-positive clones from each of rounds 1 and 2 (10 )3 dilution) and rounds 2 and 3 (10 )6 dilution) were sequenced. In all cases the sequence obtained was identical with that of the input clone from the ... FEBS 3695 In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase Wayne M. Patrick 1,2 and Jonathan M. Blackburn...

Ngày tải lên: 30/03/2014, 20:20

... 3D structure of VpUcyt in the presence of DPC micelles was calculated from a large set of proton–proton distance constraints. The topology of micelle-associated VpUcyt was derived from paramagnetic ... 6561 Biological implications of the observed VpUcyt structure One of the most studied activities of VpU is the induc- tion of CD4 degradation in the endoplasmatic reticu-...

Ngày tải lên: 18/02/2014, 06:20

... R * , ADP-ribosylated Arg102, with a mass shift of 541.06 m ⁄ z. Signals from the singly and doubly charged b- and y-ion ser- ies, as well as ions from the fragmentation of the post-translational ... 3627 MALDI-TOF analysis. This work was supported by grants to B.R H. from the Deutsche Forschungsgeme- inschaft (Re756 ⁄ 5-2) and to B.R H. and T.H. from the German Federa...

Ngày tải lên: 07/03/2014, 02:20

... strand. These interactions determine the formation of an open complex at the CT element composed of unpaired strands, CNBP, hnRNP K, and additional factors [7]. Modulation of the concentrations ... representing the apparent K d values obtained from EMSAs (means ± SEM, n ¼ 3). Band intensities from EMSAs were used as estimations of relative concentrations of binding reaction sp...

Ngày tải lên: 16/03/2014, 12:20