Báo cáo khoa học: "Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high-dose radiotherapy" pdf

... Access Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high- dose radiotherapy Luis Alberto ... Henríquez-Hernández et al.: Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance...

Ngày tải lên: 09/08/2014, 09:20

... for 10 min at 4 °C in a Sorvall RC 5B centrifuge. The pellet was slowly resuspended in NE-1 buffer containing 2.5% Triton · 100, and incubated at 4 °C with slow shaking (to lyse the chloroplast) and ... the amount of enzyme that unwinds 30% of the DNA helicase substrate at 37 °Cin30min(1 %in one min). For examining the effect of DNA- interacting compounds on DNA unwinding act...

Ngày tải lên: 20/02/2014, 11:20

... by the N-domain and C-domain varies according to the ACE species. For rat and human enzymes, estimation of IC 50 values for the binding of the inhibitor to the N-domain and C-domain can be deduced ... free of inhibitor. For each species and each inhibitor, ACE was incubated with the inhibitor for 45 min before substrate addition. The kin- etic parameters were determined using th...

Ngày tải lên: 16/03/2014, 13:20

... Over-expression of dominant-negative caveolin-1 and short hairpin RNA-mediated knock- down of caveolin-1 significantly inhibited infection without affecting the initial internalization [63]. In addition, ... receptor binding and for the induction of neutralizing antibodies. Indeed, binding sites of some neutralizing antibodies have been mapped to the C-terminal arm [15]. Under forced...

Ngày tải lên: 23/03/2014, 04:20

... IgE-binding epi- topes characterized to date in 2S albumin allergens are located in the large chain. Therefore, a common IgE epitope has been described in the large chain of 2S albumins from yellow ... signal and propeptide regions, and therefore would not be present in the mature protein, but the remainder corresponded to the large chain, with two also containing cysteine r...

Ngày tải lên: 30/03/2014, 15:20

... nigrostriatal pathway and intrinsic circuits provide the striatum with dopamine, acetyl choline, GABA, NO and adenosine. Together, these neurotransmitter systems interact with each other and with voltage-dependent ... dissolved in saline) before AlCl 3 administration; (4) L-NAME group (n = 10) - animals were treated with L-NAME with 1 single dose (1 × 10 -4 g dissolv...

Ngày tải lên: 07/08/2014, 23:22

... expression of apoptogenic protein bax, and also the release and translocation of apoptosis inducing factor AIF and cytochrome c from the mitochondria to the nucleus, without altering the subcellular distribution ... corresponds with apoptosis induction, a critical parameter impaired in cancer cells, and this induction is selectively exerted in cancer and transformed...

Ngày tải lên: 10/08/2014, 22:20

... sample was treated with proteinase K before being solubilized in digitonin-containing buffer and subjected to BN-PAGE and phosphorimaging. (B) 35 S-labeled complex IV subunits were incubated with mitochondria ... then be transferred into the maturing complex IV. Integration of nDNA-encoded subunits into supercomplexes in patient mitochondria Using digitonin solubilization of m...

Ngày tải lên: 07/03/2014, 00:20

... suggest that dietary curcumin and capsaicin individually are protective to LDL oxidation both in vivo and in vitro, to iron-induced hepatotoxi- city and to carrageenan-induced in ammation. These beneficial ... oxidation both in vivo and in vitro, to iron-induced hepatotoxicity and to carrageenan-induced in ammation. This beneficial effect was higher when the two co...

Ngày tải lên: 08/03/2014, 08:20

... removing a larger portion of the DNA. In NER, a short oligonucleotide including the damaged site is excised by nicking one DNA strand on either side of the damage and removing the intervening DNA ... licensing factor MCM2 [57,58]. Perhaps this provides a means to target histone acetylation to chromatin in the vicinity of origins in higher eukaryotes in order to regulate...

Ngày tải lên: 16/03/2014, 16:20