Báo cáo khoa học: "Gene expression profiling of human dermal fibroblasts exposed to bleomycin sulphate does not differentiate between radiation sensitive and control patients" pptx

... 6:42http://www.ro-journal.com/content/6/1/42Page 2 of 6SHOR T REPOR T Open AccessGene expression profiling of human dermal fibroblasts exposed to bleomycin sulphate does not differentiate between radiation sensitive and control patientsCharlotte ... fibroblasts exposed to bleomycin sulphate does not differentiate between radiation sensitive and control patients. Radiation Oncology 2011 6:42.Submit your next manuscript to BioMed Central and take ... Isacke2* and John R Yarnold1*AbstractBackground: Gene expression profiling of the transcriptional response of human dermal fibroblasts to in vitro radiation has shown promise as a predictive test of...

Báo cáo khoa học: Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A⁄C pot

... The amount of extracts was adjusted to obtain equal level of the various hsMOK2 proteins. The32P-labeleddouble-stranded oligonucleotide corresponds to the 18-bp MOK2binding site of human IRBP ... 3143Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A⁄CMaryannick Harper, Jeanne Tillit, Michel Kress and Miche`le Ernoult-LangeCNRS-FRE2937, Institut Andre´Lwoff, Villejuif, ... indicateddomains of hsMOK2 and the human polypeptide JLP 1–141 wereco-transformed into yeast. The speciﬁcity of the interaction between bait and prey was determined by estimating the degree of color development...

Báo cáo khoa học: Gene expression silencing with ‘speciﬁc’ small interfering RNA goes beyond speciﬁcity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx

... considered to be the best and easi-est approach to loss -of- function experiments. However, several recent stud-ies underscore the off-target and potential cytotoxic effects of siRNAs,which can lead to ... numbers of target mRNAs.Nature 433, 769–773.20 Alexopoulou L, Holt AC, Medzhitov R & Flavell RA(2001) Recognition of double-stranded RNA and acti-vation of NF-kappaB by Toll-like receptor ... is not necessary forsiRNA off-target effects. Indeed, homology between mRNA and siRNA seed region (encompassing nucleo-tides 2–8 or 2–7 of the antisense strand) was shown to be sufﬁcient to...

Báo cáo khoa học: Unconventional translation initiation of human trypsinogen 4 at a CUG codon with an N-terminal leucine A possible means to regulate gene expression pdf

... isoform (Isoform A) and notraces of the shorter isoform (Isoform B). By contrast,the expression of only Isoform B was detected in thecells transfected with the p28LT4 construct (Fig. 2B) and ... to which extracts from post mortem human brain samples did not convert the recombinantIsoform A (tag-p72-trypsinogen 4) to an Isoform B-likeprotein (Fig. 1F). Furthermore, expression of Isoform ... 4, both Isoforms A and B were detected. The exclusive interpretation of thisexperiment is that the generation of Isoform B of try-psinogen 4 occurs at the level of translation and not post-translationally.Recently,...

báo cáo hóa học:" Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers" ppt

... genes and cyclin E. The expression patterns of genes of the centromer/kinetochorecomplex and genes involved in DNA repair were similar to the expression patterns in SCC (Table 4).Corroboration of ... degree of similarity between the values. Significantly expressed genes wereuploaded to KEGG (Kyoto Encyclopedia of Genes and Genomes) and functional annotation was performed.Genes that were not ... and pulmo-nary carcinoid by gene expression profiles. Cancer Res 1999,15:5119-22.7. Hellmann GM, Fields WR, Doolittle DJ: Gene expression profiling of cultured human bronchial epithelial and...

Báo cáo y học: "Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation" pps

... Animal models of RA can facilitate the interpretation of genome-wide gene expression by providing genetic and clinical homogeneity, and an opportunity to monitor theonset and progression of the disease ... MatsuiN, Okamoto T: Gene expression profile analysis of rheumatoidsynovial fibroblast cultures revealing the overexpression of genes responsible for tumor- like growth of rheumatoidsynovium. ... regulatory network and to unravel themechanisms involved in the development and progression of autoimmune arthritis.ConclusionsThe development and progression of a complex polygenicautoimmune...

Báo cáo y học: "Gene expression profiling in the synovium identifies a predictive signature of absence of response to adalimumab therapy in rheumatoid arthritis" doc

... addition of several cytokines or combinations of cytokines: TNF-α, IL-1β,the association of TNF-α and IL-17, and the association of TNF-α and IL-1β.Materials and methodsPatients and synovial ... A-LM helped to acquire the data. BJVdE, PD, and FAH helped to design the study and contributed to the writing of the manuscript. BRL helped to design the study and to acquire, analyze, and interpret ... gene expression profiles at T0 according to thepattern of clinical response to therapy, while baseline clinical and histochemical characteristics were not different between responders and non-responders....

Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt

... induction of cytokines and adhe-sion molecules that are implicated in immune and inﬂammatory responses [24,25]. Mitogen-activatedprotein kinases (MAPKs) are important mediators of cytokine expression; ... presence of cytokines.Natural ligands of ICAM- 1and LFA-1 are expressedon the surface of T and B lymphocytes, natural killercells, monocytes, macrophages and granulocytes [33], and interaction between ... activates monocytes and macrophages to produce cytokines such as tumor necrosis factor- a and interleukins IL-1b and IL-6. These cytokines appear to be responsible for the neurotoxicityobserved...

Báo cáo khoa học: Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana ppt

... mixture of the two types of probes. For analysis of differential gene expression, 1473 and 1703 beads were collected in fractions U1 and U2 of the up-regulated genes, and 3550 beads infraction D of ... i.e., enhancement of the refolding and degradation of unfolded proteins, attenuation of translation [20,21], and apoptosis [24]. ER stress has not been found to cause attenuation of translation ... appeared to have a strategy for overcomingER stress through enhancement of protein folding activity, degradation of unfolded proteins, and regulation of apoptosis, but not regulation of trans-lation.AbbreviationsAARE,...

Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

... suggests a nonubiquitous expression (Table 1 shows a human ⁄ mouse comparison togetherTable 1. Comparison of human and mouse STK33 ⁄ Stk33 expression levels between our data and expression databases.Organ ... theWeizmann Institute of Science (http://bioinformatics.weizmann.ac.il/cards), which is restricted to human genes and does not provide results from testis, shows expression of STK33 in lung and heart. ... signals of Stk33 in certain stages of sperma-togenesis, in some embryonic fetal organs and itsinvolvement in child diseases, let us to postulate a rolein gametogenesis and organ ontogenesis...

Xem thêm