Báo cáo y học: "Antiphospholipid reactivity against cardiolipin metabolites occurring during endothelial cell apoptosis" doc
Báo cáo y học: "Antiphospholipid reactivity against cardiolipin metabolites occurring during endothelial cell apoptosis" doc
... NHS indicates healthy donors. (a) anticardiolipin reactivity; (b) antimonolysocardiolipin reactivity; (c) antidilysocardiolipin reactivity; (d) anti-hydrocardiolipin reactivity. The average immunoreactivities ... (a, b). aCL, anticardiolipin antibody; aDCL, antidilysocardiolipin antibody; aHCL, antihydrocardiolipin antibody; aMCL, antimonolysocardiolipin antibody; OD, optical density.Available online ... hybrid proper-ties between a diacyl-lipid (bilayer-forming) and a lyso-lipid(micelle-forming), which may physically facilitate autoantibody reactivity. In contrast, there are fundamental physico-chemicalreasons...
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Báo cáo y học: "Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation" doc
... and phenotypic analysis by flow cytometry.Numbers of CD45+ cells increased during the first 6 days, withincreases in CD45+MHC (major histocompatibility complex) II+monocyte-like cells from ... inflammatory cells obtained from SRT by enzymaticdigestion (Figure 3c). Importantly, they show that Mϕ are anumerically small subset of the total CD45+ cells in theinflamed synovial tissues during ... inflammatory mono-cytes did not increase until days 9 to 12 and days 12 to 14,respectively. The delayed appearance of MHC II- cells (mainlyPMN cells), which comprised approximately 92% ofCD45+MHC...
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Báo cáo y học: "Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain"
... administered intrathecally once daily for 7 days, starting from 1 day before CCI surgery. Evaluation of tactile allodynia and thermal hyperalgesia The paw withdrawal latency (PWL) to radiant heat ... microscope and the fluorescence intensity was quantified by flow cytometry. To identify the knockdown efficacy of different siRNA oligonucleotides, HEK-293 cells were cotrans-fected with pEGFPC1-TLR4 ... quantification of TLR4-EGFP fluorescence intensity upon siRNA knockdown was evaluated by flow cytometry analysis. Immunofluorescence and flow cytometry results revealed that all 3 siRNAs had efficient...
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Báo cáo Y học: IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1 docx
... ELISA reactivity, IgE antibodybinding, and skin test reactivity. J. Allergy Clin. Immunol. 107,S219.26. Romanos, M.A., Makoff, A.J., Fairweather, N.F., Beesley, K.M.,Slater, D.E., Rayment, ... media byaffinity chromatography over a 10A6 monoclonal antibodycolumn as described previously [11] with modifications.Bound rBla g 1 was eluted with 0.05Mglycine in 50%ethylene glycol, pH ... cockroach allergens: analysis with monoclonal antibody-basedenzyme immunoassays. J. Allergy Clin. Immunol. 87, 505–510.16. Finn, P.W., Boudreau, J.O., He, H., Wang, Y. , Chapman, M.D.,Vincent,...
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Báo cáo Y học: The reactivity of a-hydroxyhaem and verdohaem bound to haem oxygenase-1 to dioxygen and sodium dithionite pdf
... ferrouscomplex was mainly formed through reduction of freea-hydroxyhaem by dithionite followed by its direct bindingto the enzyme, rather than by reduction of the enzyme-boundferric a-hydroxyhaem, as no ... Chemistry,Vol. 51 (Sykes, A.G., ed.), pp. 359–407. Academic Press, SanDiego.16. Davydov, R.M., Yoshida, T., Ikeda-Saito, M. & Hoffman, B.M.(1999) Hydroperoxy-heme oxygenase generated by cryoreductioncatalyzes ... Biochemistry, Kurume University School of Medicine, Japan;2Department of Biochemistry and Cell Biology,Rice University, Houston, Texas, USARecently we have shown that ferric a-hydroxyhaem boundto...
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Báo cáo y học: "Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia" ppt
... checked by indirect en-zyme-linked immunosorbent assay (ELISA), myeloma cells line SP2/0 (106) were fused with splenocytes (107) by the addition of 45% polyethylene glycol (PEG-4000). Hybridomas ... detection by immunocytochemistry. Blood. 2002; 99: 409-26. 17. Falini B, Mecucci C, Tiacci E, et al. Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype. N Engl ... and antibody production were subjected to subcloning. Antibodies secreted by the 2G3 clones were found to be IgG iso-type. The specificity of the mAbs against NPM-mA was assessed by indirect...
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Báo cáo y học: "Antiphospholipid syndrome" pot
... serine proteases [19].Cellular activation by β2GPI–antibody complex in APSβ2GPI–antibody complex is able to bind several cell types,such as endothelial cells, monocytes, and platelets. ... thromboticmicroangiopathy, such as thrombotic thrombocytopenicpurpura or hemolysis, elevated liver enzymes, and low plateletcount syndrome [53]. There is usually accompanying hemo-lytic anemia, often ... measures. Prophylaxiswith heparin administered subcutaneously should certainly begiven to cover higher-risk situations, such as surgery.Moreover, hydroxychloroquine may be protective against thedevelopment...
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Báo cáo y học: "Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice" pps
... RA, inflammatorycytokines, chemokines, growth factors and adhesion mole-cules are produced by leukocytes and resident synoviocytes.These factors perpetuate chronic inflammation by the recruit-ment ... PARP-1 activa-tion induced by genotoxic injury developed during theCAIA = collagen antibody-induced arthritis; Ccl5 (RANTES) = small inducible cytokine A5; COX-2 = cyclooxygenase; DMEM = Dulbecco's ... = 3,4-dihydro-5- [4-(1-piperidinyl)butoxy]-1(2H)-isoquinolinone; FLS = fibroblast-like synoviocyte; H&E = hematoxylin and eosin; IL = interleukin; iNOS = inducible nitric oxide synthase;...
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Báo cáo y học: "Human autoantibodies against the 54 kDa protein of the signal recognition particle block function at multiple stages" ppt
... 5–15 is from a polymyositis patient without detect-able myositis autoantibodies, and serum 1–24 is from a polymyositis patient with autoantibodies directed against histidyl-tRNA synthetase. PPL, ... from the SRP54M-domain.IntroductionBoth secretory and membrane proteins destined for entry intothe eukaryotic secretory pathway carry hydrophobic signalsequences that direct them to the ... previous study ofmyositis autoantibody inhibition of autoantigen function foundthat all six of the anti-aminoacyl-tRNA synthetase antibodiesthat were tested inhibited enzymatic activity [43].The...
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Báo cáo y học: "Enhanced reactivity to pain in patients with rheumatoid arthritis" ppsx
... multipletypes of stressors on the physiology and symptomatology ofpatients with RA. Collectively, laboratory research has docu-mented a maladaptively pro-inflammatory response to stressamong RA ... natu-ral killer cell cytotoxicity in humans and this response is abol-ished by local anaesthesia. Br J Anaesth 1999, 83:235-240.48. Kurtais Y, Tur BS, Elhan AH, Erdogan MF, Yalcin P: Hypotha-lamic-pituitary-adrenal ... study, we compared psychophysical and physiologicalresponses to standardized noxious stimulation in 19 RA patientsand 21 healthy controls.Methods Participants underwent a single psychophysicaltesting...
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