Báo cáo y học: "Antiphospholipid reactivity against cardiolipin metabolites occurring during endothelial cell apoptosis" doc

... NHS indicates healthy donors. (a) anticardiolipin reactivity; (b) antimonolysocardiolipin reactivity; (c) antidilysocardiolipin reactivity; (d) anti- hydrocardiolipin reactivity. The average immunoreactivities ... (a, b). aCL, anticardiolipin antibody; aDCL, antidilysocardiolipin antibody; aHCL, antihydrocardiolipin antibody; aMCL, antimonolysocardiolipin antibody; OD, optical density....

Ngày tải lên: 09/08/2014, 08:23

... and phenotypic analysis by flow cytometry. Numbers of CD45 + cells increased during the first 6 days, with increases in CD45 + MHC (major histocompatibility complex) II + monocyte-like cells from ... inflammatory cells obtained from SRT by enzymatic digestion (Figure 3c). Importantly, they show that Mϕ are a numerically small subset of the total CD45 + cells in the inflamed synovial tissu...

Ngày tải lên: 09/08/2014, 10:22

... administered intrathecally once daily for 7 days, starting from 1 day before CCI surgery. Evaluation of tactile allodynia and thermal hyperalgesia The paw withdrawal latency (PWL) to radiant heat ... microscope and the fluorescence intensity was quantified by flow cytometry. To identify the knockdown efficacy of different siRNA oligonucleotides, HEK-293 cells were cotrans- fected with...

Ngày tải lên: 25/10/2012, 11:48

... ELISA reactivity, IgE antibody binding, and skin test reactivity. J. Allergy Clin. Immunol. 107, S219. 26. Romanos, M.A., Makoff, A.J., Fairweather, N.F., Beesley, K.M., Slater, D.E., Rayment, ... media by affinity chromatography over a 10A6 monoclonal antibody column as described previously [11] with modifications. Bound rBla g 1 was eluted with 0.05 M glycine in 50% ethylene glycol, pH ... c...

Ngày tải lên: 08/03/2014, 23:20

... ferrous complex was mainly formed through reduction of free a-hydroxyhaem by dithionite followed by its direct binding to the enzyme, rather than by reduction of the enzyme-bound ferric a-hydroxyhaem, as no ... Chemistry, Vol. 51 (Sykes, A.G., ed.), pp. 359–407. Academic Press, San Diego. 16. Davydov, R.M., Yoshida, T., Ikeda-Saito, M. & Hoffman, B.M. (1999) Hydroperoxy-heme oxygenase gene...

Ngày tải lên: 23/03/2014, 21:20

... checked by indirect en- zyme-linked immunosorbent assay (ELISA), myeloma cells line SP2/0 (10 6 ) were fused with splenocytes (10 7 ) by the addition of 45% polyethylene glycol (PEG-4000). Hybridomas ... detection by immunocytochemistry. Blood. 2002; 99: 409-26. 17. Falini B, Mecucci C, Tiacci E, et al. Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype. N...

Ngày tải lên: 08/08/2014, 18:21

... serine proteases [19]. Cellular activation by β 2 GPI–antibody complex in APS β 2 GPI–antibody complex is able to bind several cell types, such as endothelial cells, monocytes, and platelets. ... thrombotic microangiopathy, such as thrombotic thrombocytopenic purpura or hemolysis, elevated liver enzymes, and low platelet count syndrome [53]. There is usually accompanying hemo- lytic anem...

Ngày tải lên: 09/08/2014, 01:22

... RA, inflammatory cytokines, chemokines, growth factors and adhesion mole- cules are produced by leukocytes and resident synoviocytes. These factors perpetuate chronic inflammation by the recruit- ment ... PARP-1 activa- tion induced by genotoxic injury developed during the CAIA = collagen antibody-induced arthritis; Ccl5 (RANTES) = small inducible cytokine A5; COX-2 = cyclooxygenase; DMEM =...

Ngày tải lên: 09/08/2014, 07:20

... 5–15 is from a polymyositis patient without detect- able myositis autoantibodies, and serum 1–24 is from a polymyositis patient with autoantibodies directed against histidyl-tRNA synthetase. PPL, ... from the SRP54 M-domain. Introduction Both secretory and membrane proteins destined for entry into the eukaryotic secretory pathway carry hydrophobic signal sequences that direct them to the .....

Ngày tải lên: 09/08/2014, 07:20

... multiple types of stressors on the physiology and symptomatology of patients with RA. Collectively, laboratory research has docu- mented a maladaptively pro-inflammatory response to stress among RA ... natu- ral killer cell cytotoxicity in humans and this response is abol- ished by local anaesthesia. Br J Anaesth 1999, 83:235-240. 48. Kurtais Y, Tur BS, Elhan AH, Erdogan MF, Yalcin P: Hypo...

Ngày tải lên: 09/08/2014, 14:20