Báo cáo y học: "Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reacti" potx

... 1 of 9 (page number not for citation purposes) Vol 8 No 6 Research article Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction Dylan ... followed by 40 cycles of 95°C for 1 minute and 60°C for 15 seconds, as recommended by the manufacturer (Applied Biosystems). Real-time data were ana- lysed by us...

Ngày tải lên: 09/08/2014, 08:22

... degree of staining in KO MRL mice and CTRL MRL mice as measured by serial dilution of antibody or by scoring of representative glomeruli by blinded observers (P = 0.55 and P = 1.0, respec- tively; ... is characterized by lymphadenopathy, complement activation, severe immune complex renal disease, and 50% lethality by 20–24 weeks of life [35]. We report here that C4bp d...

Ngày tải lên: 09/08/2014, 10:21

... 6271 dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitina- tion of Lys, and phosphorylation of Ser and Thr [16– 18]. Combinations of these ... identify phosphophoryla- tion sites were limited to analysis of tryptic peptides by low-energy CAD. In an earlier study, a yeast whole cell lysate was proteolytically digested...

Ngày tải lên: 18/02/2014, 16:20

... University. Genetic analyses DNA was extracted by the salting-out method described by Miller and colleagues [18]. Analysis of genetic polymor- phism was predominantly performed by polymerase chain reaction ... lymphocytotoxicity test or by restriction fragment length polymorphism as described before [2]. C4A gene deletion was determined by PCR as described by Grant and c...

Ngày tải lên: 09/08/2014, 01:24

... analyses of the frequency of occurrence and mutations of the highly mutable motifs RGYW and WRCY revealed that mutations in nonproductive V L rearrange- ments of B cells from the parotid gland ... 4%) but not by non- productive rearrangements of the gland and by productive rearrangements of the peripheral blood (Jλ1, 0%; Jλ7, 7%) and the parotid gland (Jλ1, 0%; Jλ7, 7%...

Ngày tải lên: 09/08/2014, 03:24

... cultural examination of common bacteria and fungi, and of direct acid-fast bacilli smears (Kinyoun method) and cultures for mycobacteria, and for the microscopic examination of Pneumocystis jiroveci ... represen- tation, and σ x and σ y were the SD of the spot's Gaussian dis- tribution in the directions of the x and y axes, respectively) normalized the value...

Ngày tải lên: 09/08/2014, 08:22

... Schumacher H, Arayssi T, Branigan P, Gerard HC, Klippel J, Pando J, Getsy J, Bulbul R, Yarboro C, Rothfuss S, et al.: Survey- ing for evidence of synovial Chlamydia trachomatis by polymerase chain reaction ... under- standing of the contribution made by intestinal microflora to human biology is needed to elucidate the potential role played by microflora in the pathogenesis of...

Ngày tải lên: 09/08/2014, 10:23

... polymorphism that is tagged by some haplotypes containing the T allele of rs582757 (Table 2). No significant change was detected by stratifying by sex (data not shown) or by the presence of rheumatoid ... the study and in the coordination of acquisition of clinical data and collection of samples, and supervised genotyping, statistical analysis, inter- pretation of re...

Ngày tải lên: 09/08/2014, 14:20

... AITDs (n = 145) and age-matched healthy women (n = 257). XCI analysis was performed by enzymatic digestion of DNA with a methylation sensitive enzyme (HpaII) followed by PCR of a polymorphic CAG ... from 10 ml of peripheral blood lymphocyte of patients and controls using standard methods [16]. Genotyping of a polymorphic site in the androgen recep- tor (AR) gene was performe...

Ngày tải lên: 09/08/2014, 14:22

... sample of 153 exon-skipping events predicted by the microarray analysis, 73 were successfully validated by RT- PCR and sequencing (a 48% validation rate). This initial study has made a very substantial ... together by an alternative splicing event. Alternative splicing also poses new challenges for microarray data analysis. The overall expression level of a gene can be represen...

Ngày tải lên: 09/08/2014, 20:20