... NirF, which aligns with His200 of the d1heme domain of cd1, is essential both for this binding and for theproduction of d1heme; replacement of His41 by Ala, Cys, Lys and Met allgave nonfunctional ... Expression of NirF in Paracoccus pantotro-phus DnirF strain.Fig. S2. Multiple sequence alignment of NirF with d1domain of cd1, NirS.Fig. S3. Sequence alignment of NirF with Met8p and CysG.Fig. ... number of mutationsusing the following primer combinations on both thepET22b- and pEG276-based clones: H41A – SB82 and SB83, H41K – SB114 and SB115, H41C – SB116 and SB117,H41M – SB118 and SB119,...