Báo cáo y học: "Analog peptides of type II collagen can supp

Báo cáo y học: "Analog peptides of type II collagen can suppress arthritis in HLA-DR4" docx

... response to type II collagen. A panel of analog peptides containing amino acid substitutions was tested for the ability to stimulate the proliferation of type II collagen (CII)-primed T cells. ... containing substitutions within the immunodominant determinant of type II collagen that effectively suppress autoimmune arthritis in DR4 transgenic mice. Both of...

Ngày tải lên : 09/08/2014, 08:22

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Báo cáo Y học: The refolding of type II shikimate kinase from Erwinia chrysanthemi after denaturation in urea pot

... means of investigating the integrity of this region of the protein. In the present paper, we have undertaken a study of the unfolding and refolding of the type II SK from E. chrysanthemi, using ... of signiﬁcant contaminant by nucleotide. Assay of enzyme activity The activity of the shikimate kinase was determined by a double coupled assay involving pyruvate kinase (PK...

Ngày tải lên : 08/03/2014, 23:20

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Báo cáo y học: "Delayed resolution of acute inflammation during zymosan-induced arthritis in leptin-deficient mice" pptx

... we investigated the effects of leptin deficiency on zymosan-induced arthritis (ZIA), which is independent of adaptive immunity. Arthritis was induced by injection of zymosan into the knee joint. ... joint swelling in zymosan-induced arthritis Arthritis was induced in ob/ob and control +/? C57BL/6 mice by injecting zymosan A into the right knee joint. To assess whether leptin...

Ngày tải lên : 09/08/2014, 01:23

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Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"

... immunoglobulin chains were also found to be significantly increased in aged thymocytes. Understanding the changes in gene expression in thymocytes with age may hold the key in determining thymocyte ... thymus, involution, aging, microarray, AGEMAP, thymocytes, caloric restriction Introduction The aging immune system is often characterized by a general decline in the ability to...

Ngày tải lên : 03/11/2012, 11:35

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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot

... recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing the coding sequence ... the C-terminus of CCR5 would interfere with the protein’s ability to function as a chemokine receptor or HIV coreceptor, we ﬁrst determined the binding of MIP-1a to the CCR5-myc-his expr...

Ngày tải lên : 08/03/2014, 09:20

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Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot

... access [14]. In contrast to all Fe-hydrogenases including HydA of S. obliquus,the enzyme of C. reinhardtii has an interesting additional protein domain. A small insertion of 45 amino acids between ... synthesis of s ome enzymes involved in glycolysis or sugar-phosphate metabolism [54]. HydA belongs to a new class of Fe-hydrogenases HydA of C. reinhardtii, the ﬁrst isolated gen...

Ngày tải lên : 08/03/2014, 22:20

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Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf

... expression of a variety of single mitochondrial -type AACs in E. coli. After IPTG-induction we were able to obtain functional integration of the various mitochondrial -type AACs into the cytoplasmic ... trans- ferredtonewreactionvesselsandheatedto95°Cfor 10 min to prevent any further reaction. An aliquot of the exported radioactive solution was used for separation by thin-layer ch...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Supermolecular organization of photosystem II and its associated light-harvesting antenna in Arabidopsis thaliana docx

... packing of the supercomplex is very similar to the packing in the crystals, as is shown in the ﬁtting of crystal features into the spinach type III megacomplex (Fig. 4F). Finally, the CP26 area in ... addition was not performed in the analysis of the spinach crystals, where crystallinity in one speciﬁc part of a layer did not strongly correlate to crystallinity in the ot...

Ngày tải lên : 24/03/2014, 04:21

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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

... of cofactor for TDP enzymatic activity was examined by determining the speciﬁc activity of TDP by following the cleavage of 1 m M of substrate by 0.125 l M of enzyme in reaction buffer containing ... linking the active site tyrosine residue of topoisomerase I with the 3¢ terminus of DNA in topoisomerase I–DNA complexes which accumulate during treatment of cancer with cam...

Ngày tải lên : 31/03/2014, 21:21

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Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/triﬂuoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot

... a 1 Gly56 2.6H Tyr50 a 2 Gly56 3.5H Tyr50 a 2 Gly56 2.6H Tyr50 a Ala59 3.5H Tyr50 a Ala59 2.6H Tyr50 b Ala59 3.5H Tyr50 b Ala59 b 1 Tyr50 a 1 Gly56 b 2 Tyr50 a 1 Gly56 b 1 Tyr50 a 2 Gly56 b 2 Tyr50 ... during the early stages of HIV-1 replication, binding of NCp7 to the C-terminal domain (80–96)Vpr [8] may expose the N-terminal (1–39) portion of the protein and allow its tight binding t...

Ngày tải lên : 31/03/2014, 23:20

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