Báo cáo y học: "tatin-induced expression of CD59 on vascular endothelium in hypoxia: a potential mechanism for the anti-inflammatory actions of statins in rheumatoid arthritis" pptx
Báo cáo y học: "tatin-induced expression of CD59 on vascular endothelium in hypoxia: a potential mechanism for the anti-inflammatory actions of statins in rheumatoid arthritis" pptx
... and pros-Figure 6Hypoxia increases atorvastatin-induced decay-accelerating factor expressionHypoxia increases atorvastatin-induced decay-accelerating factor expression. Analysis of decay-accelerating ... 7Atorvastatin-induced CD59 and decay-accelerating factor in hypoxia enhance endothelial cell cytoprotectionAtorvastatin-induced CD59 and decay-accelerating factor in hypoxia enhance endothelial ... hypoxic conditions may contribute to an anti-inflamma-tory action of statins in RA. The combined effects of DAF, at the level of C3 and C5 convertases, and of CD59 inhibiting the terminal attack...
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Báo cáo y học: "Granulocyte-CSF induced inflammation-associated cardiac thrombosis in iron loading mouse heart and can be attenuated by statin therapy" pptx
... Harada M, Qin Y, Takano H, Minamino T, Zou Y, Toko H, Ohtsuka M,Matsuura K, Sano M, Nishi J, Iwanaga K, Akazawa H, Kunieda T, Zhu W,Hasegawa H, Kunisada K, Nagai T, Nakaya H, Yamauchi-Takihara ... Hornung CA, Dawn B:Granulocyte colony-stimulating factor therapy for cardiac repair afteracute myocardial infarction: a systematic review and meta-analysis of randomized controlled trials. Am Heart ... of inflammatory signaling in the heart tissue.Elevated pAkt and eNOS expression in simvastatinsupplemented heartsTo elucidate the molecular pathway of statin’ santi-inflammation therapy on...
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Báo cáo y học: "ene expression analysis of human red blood cells"
... in human RBCs encoding initiation, activation and regulation of transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD finger- DNA-binding proteins, cysteinyl, lysyl-tRNA ... findings. But gene expression profiling of human erythrocyte could be an important key for under-standing the machinery of anucleate protein synthesis and its meaning in the pathophysiology ... a protein synthesis in human platelets by megakaryo-cyte-derived mRNAs 19. The finding of RNA in anu-cleate cells like erythrocytes support the idea of nu-cleus independent protein synthesis...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... most cystatins inhi-biting cathepsin L also inhibit cathepsin S, an inhibitoryactivity of cystatin F against cathepsin S has not yet beenreported. The expression of the cystatin F (CST7)andC(CST3)genes ... [5] a totalabsorption of the signal was apparent.Fig. 6. Double staining of cystatin F and ER. (A) Cystatin F was stained with a humancystatin F specific polyclonal antiserum and a FITC labelled ... tofractionate cells to determine the localization of the inhibitor. Initially cells were fractionated in a debris fraction(containing nuclei and cell debris), a heavy and a lightfraction containing...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... b-Glucosidase-containing fractions were pooled and checked for purityby SDS/PAGE.Enzyme assaysFractions g enerated during isolation of CBG from humanliver were assayed for CBG a ctivity u sing a ... largelythrough an increase in kcat(Table 2). CBG was tested for activity against a series of ¯avonol glycosides that differedonly in the glycone moiety [Q3Glu, Q3Gal, Q3Xyl, Q3Ara,Q3GlA, Q3Rha and Q3GlcMal; ... (1997) The crystal structures of Sinapis alba myro-sinase and a covalent glycosyl±enzyme intermediate provideinsights into the substrate recogn ition and active-site machinery of an S-glycosidase....
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Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx
... between the IgA and SClevels in all the clones demonstrated that a sufficient amount of J-chain was available for SIgA complex formation. OneSIgA-producing clone (SIgA-3) along with pIgA-D andIgA-29 ... continuous stirring for 1 min.An additional 3 mL of prewarmed medium was added the same way. The fusion mixture was then slowly mixed with10 mL of prewarmed medium and incubated for 5 min. The cells ... absorbance was read byTitertekÒ Multiskan (ICN Flow, USA). The amount of IgA present in each supernatant was calculated relative to a standard preparation with known concentration.Verification of...
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... National de la Recherche Scientifique, Gif-sur-Yvette, FranceMammalian tubulins and actins attain their native con-formation following interactions with CCT (the cytosolicchaperonin containing ... reaction products wereanalyzed by nondenaturing PAGE; the ratios of labeled to unlabeledtubulins are indicated on the top of each lane and the arrow indicates the position of the b-tubulin/CCT ... apical domain of the CCT ring[9,10] in a nearly native conformation [10,11]. Substratebinding to CCT is accompanied by a change in the conformation of CCT [9,10,12]. Nucleotide exchange andhydrolysis...
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Báo cáo Y học: Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids pot
... results in the same end product cathenamine and involves the sameintermediate 4,21-dehydrocorynantheine aldehyde. The formation of the 3-isocorreantine A after hydrolysis of dolichantoside is an indication ... in uencing the biosynthesis on a rational basis. The best known pathways comprise those of the flavonoid biosynthesis [1,2], the isoquinoline alkaloidformation [3,4] and the biosynthesis of indole alkaloids ... vincaleucoblastineand vincristine used in cancer chemotherapy, the toxinstrychnine, the vasodilative yohimbine, the neurolepticreserpine, the antihypertensive ajmalicine and the anti-arrhythmic...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... arehazardous and can result in environmental contamination.So the conversion of nitro groups by biocatalysts is useful in industry as well as in environmental conservation. Enzymesthat can convert ... indicated the production of nitrite, was seen in assays containing nitromethane (Fig. 3). In addition to nitrite formation, carbonyl compoundsreleased in the oxidation of nitroalkanes by NaoA weredetermined ... recombinant NaoAafter DEAE-Sepharose Fast Flow chromatography; lane 6, standardmolecular mass markers (phosphorylase b,97kDa;BSA,66kDa;ovalbumin, 45 kDa).Fig. 3. Assay of NaoA activity. (A) ...
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Báo cáo y học: "Quantitative expression of osteopontin in nasal mucosa of patients with allergic rhinitis: effects of pollen exposure and nasal glucocorticoid treatment" pdf
... expression of OPN in the cells of the nasal mucosa of AR patientsdoes not increase over a natural pollen season, althoughan increase in several cytokines and cells in the nasalmucosa over the pollen ... other healthy tissues [7], our data pro-vides the first example of the distribution in the nasalmucosa of AR patients. The expression of OPN protein in the nasal mucosa of healthy and AR subjects ... using quantitative imagingsoftware (Leica QWin) with the same threshold used for all sections. The data was expressed as a percentage of total tissue area.GraphPad 5 (GraphPad Software, Inc....
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