Báo cáo y học: "Inhibition of protein geranylgeranylation induces apoptosis in synovial fibroblasts" pdf

... restores the viability of synovial fibroblasts in the presence of statinsGeranylgeranylpyrophosphate (GGPP) restores the viability of synovial fibroblasts in the presence of statins. Synovial fibroblasts ... in viability in the presence of statins. Geranylgeranylpyrophosphate restores the viability of RA synovial fibroblasts in the presence of statins The dec...

Ngày tải lên: 09/08/2014, 08:22

... respiration in the presence of oligomycin of L6 cells showed that they were very well coupled. The oligomycin resistant respiratory activity of the cells could be strongly stimulated by the addition of ... that of UCP1) specifically interact with members of the 14.3.3 protein family [37]. The 14.3.3 proteins are located in the cytosol where they regulate various aspects o f c...

Ngày tải lên: 21/02/2014, 15:20

... Representative micrographs of this grading system are presented in Figure 1. Quantification of inflammatory cytokines in synovial fluid Cytokine profiles in synovial fluid were determined using a BD™ cytometric ... stop taking aspirin and all cytokine inhibitors 10 to 14 days prior to surgery. Histology of synovial biopsies and levels of cytokines Haematoxylin and eosin h...

Ngày tải lên: 09/08/2014, 10:23

... mediators of tis- sue destruction in many immunoinflammatory diseases such as RA [1-3]. The two cytokines induce, in synergy, the production of high levels of matrix metalloproteinases by synovial ... E 2 , whereas interleukin-1β, a COX-2 inducer, strongly increased diosgenin- induced apoptosis of these synoviocytes. These findings suggest that the proapoptotic effect of dio...

Ngày tải lên: 09/08/2014, 01:23

... mediated, at least in part, by G -protein coupled receptors (GPCRs), stimulation of membrane phospholipid hydrolysis, and activation of the diacylglycerol sensitive isoforms of protein kinase C (PKC) [28]. ... inhibition of ERK1/2 phosphorylation may be lying upstream of ERK1/2. Using a serine/threonine kinase SPA assay kit, the capability of NAMI-A to affect the ERK1/2 phosp...

Ngày tải lên: 21/02/2014, 01:21

... splicing apparatus. The inhibitory effect of erythromycin and Cl-tetracycline were not allevi- ated by Mg 2+ (Fig. 3). Kinetic analyses using increasing concentrations of erythromycin, Cl-tetracycline ... was reduced by 60% in the presence of 500 l M Cl-tetracycline and by 95% in t he presence of 500 l M erythromycin and streptomycin (Fig. 2B,C). Tetracycline at 500 l M inhibited...

Ngày tải lên: 21/02/2014, 15:20

... some of the complexes or by other experimental means. CONCLUSIONS We have analysed the possibility of predicting the residues forming part of protein protein interacting surfaces in proteins of ... recently, methods were devised for predicting residues involved in protein interaction s ites in the absence of any structural reports. By analysing hydrophobicity distribution,...

Ngày tải lên: 21/02/2014, 15:20

... level of the has operon containing the hyaluronan synthase gene or the protein level of hyaluronan synthase. Solubilization of the enzyme from membranes of MU-treated cells and addition of the ... protein utilizing the principle of protein- dye binding. Anal. Biochem. 72, 248–254. 32. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of...

Ngày tải lên: 08/03/2014, 09:20

... activity of a number of key enzymes such as cyclooxygenase [9], protein kinase C [10], protein tyrosine kinases [11] and a Ca 2þ -dependent endonuclease [12]. Many of these processes/enzymes are ... processes including: activation of apoptosis in Jurkat T-cells [5], inhibition of platelet aggregation [6,7] and inhibition of in ammatory cytokine production in macrophages...

Ngày tải lên: 08/03/2014, 23:20

... transduction in macrophages by glycosylphosphatidylinositols of Plasmodium, Trypanosoma, and Leishmania: activation of protein tyrosine kinases and protein kinase C by inositolglycan and diacylglycerol moieties. ... organisms. In all systems investigated so far, mannosamine was able to inhibit GPtdIns biosynthesis. In T. brucei, mannosamine inhibits the incorporation of ethano...

Ngày tải lên: 31/03/2014, 23:20