Báo cáo y học: "Expression of bioactive bone morphogenetic proteins in the subacromial bursa of patients with chronic degeneration of the rotator cuff" pptx
Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx
... zinc-containing protein with the zinc finger or zinc cluster exist in yeast, which, however, lacks proteins with the hormone receptor-type zinc-binding motif. This type of zinc-binding proteins ... co-ordination sphere [3]. After the finding of a number of zinc-containing DNA- binding proteins in higher eukaryotes, many different types of the zinc-binding motif hav...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... be quickly separated from the bulk of other bacterial proteins by using metal chelate affinity chromatog- raphy [11]. Because N-terminal His-tag may influence the function of protein, we designed ... present in inclusion body. In order to recover its function, the fusion protein in inclusion body was refolded in urea gradient refolding buffer. And then, the refolded p...
Ngày tải lên: 25/10/2012, 11:10
Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
... counterstained with Meyer’s haematoxylin and mounted in Permount TM . Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized ... epithelial layers of reticular subtype of oral lichen planus patient (streptavidin-biotin amplified system, x 400). Figure 2. Increased labelling of hMSH2...
Ngày tải lên: 03/11/2012, 09:54
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... protein as judged by SDS/ PAGE. The activity of the pure recombinant protein was 80% of that of t he wild-type protein in both the lysozyme and chitinase assays. Attempts to further purify the recombinant ... to the formation of the oxazolinium intermediate in concert with Asp125, but not to the binding of the substrate. Comparison of our kinetic data with...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
... feasible explanation for the complementability of the group 2 proteins for the yeast vma3-mutant. A comparison of the structures, especially in the four hydrophobic domains (underlined sequences in Fig. 1A), ... induction, accumulation of the dye in the vacuoles of both transformants was observed, and the intensity was weaker than that in the transformants...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
... polypep- tides, all the proteins of interest were present in the soluble fraction of the induced cell in the case of the expression of pET22b(+)/touBEA and /touC, whereas the product of the expression of pET22b(+)/touF ... functional. To identify the hydroxylase component of the complex we performed single-turnover assays, in the absence of the To...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
... conserved using BLAST analysis comparing all of the vitamin K-dependent carboxylases. In contrast, the C-terminal 25% of the protein shows no homology to any of the other vitamin K-dependent carboxylases. Fig. ... doi:10.1046/j.1432-1033.2002.03335.x by the addition of proFIX18 and proPT18, indicating that the Conus carboxylase, like the bovine carboxylase, is activate...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx
... be the start of the protein coding domain. Sequence analysis of the 3¢ end cDNA revealed that there is a polyadenylation signal upstream of the poly(A). Analysis of the primary structure of M. ... 2002 Recently, the a subunit of a G protein of the Gq family has been immunolocalized in olfactory sensilla preparations of the silkmoth Antherea pernyi [23]. Using...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx
... penaeidins. These observations argue in favour of a coating, by released penaeidin, of the bacteria before their internalization into penaeidin-devoid hyaline cells. Finally, the diversity, the large ... defensins [38] or in mussel haemocytes for mytilins [17]. The question remains about the function of these intracellular penaeidins and their potential involvement in t...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx
... obtained on the same gel. The density of UCP3 protein (i.e. the intensity of the signal expressed per mg of proteins) was similar in the SS mitochondria of the two glycolytic muscles, tibialis anterior and ... secondary anti- body. The signals were detected by chemiluminescence using a standard ECL kit and developed Hyperfilm ECL film. They were quantified by scanning p...
Ngày tải lên: 24/03/2014, 04:21