Báo cáo y học: "Human, viral or mutant human IL-10 expressed after local adenovirus-mediated gene transfer are equally effective in ameliorating disease pathology in a rabbit knee model of antigen-induced arthritis" ppt
Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... group maintained at 37oC. Additionally, normal karyotype was maintained in all low temperature-exposed groups, as assessed by fluorescence in situ hybridization (FISH) of metaphase spreads with ... (1–22 and X and Y) is painted a different color, using combinatorial labeling, so that any interchromosomal translocations are observed as color junctions on individual chromosomes. Painting ... effects, the same cannot be said of human embryos, which are much less robust and hardy compared to mouse embryos. Hence laboratories involved in handling human embryos in clinical assisted reproduction...
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Báo cáo y học: "ene expression analysis of human red blood cells"
... biological meaning of these findings. But gene expression profiling of human erythrocyte could be an important key for under-standing the machinery of anucleate protein synthesis and its meaning ... DNA-binding proteins, cysteinyl, lysyl-tRNA syn-thetase), important RNA-stabilising factor - poly (A) binding protein, anti-apoptotic proteins (for instance beclin 1, reticulon 4, BCL2, IAP) ... with genes for RNA degradation (for example ribonuclease T2) as well as genes encoding typical apoptotic proteins such as cyclooxygenase, apoptotic protease activating fac-tor, caspase 8....
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Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
... Arias, I.M. (1990) Serial quanti-tative image analysis and confocal microscopy of hepatic uptake,intracellular distribution and biliary secretion of a fluorescentbile acid analog in rat hepatocyte ... twoYb3+bind to apo-Tf in the specific iron binding site and twotyrosines are involved in binding of Yb3+ in both the N- andC-lobe as the case for Fe3+. The X-ray crystal structure of Sm2-lactoferrin ... (1999)Biologicaleffectofrareearth(I)-contentanddistributionofrareearth in normal human plasma. Chem.J.Chin.University20, 5–8.14. Luk, K.C. (1971) Study of the nature of the metal-binding sitesand estimate of...
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Báo cáo Y học: The a1b1 contact of human hemoglobin plays a key role in stabilizing the bound dioxygen Further evidence from the iron valency hybrids potx
... University, Hiroshima, Japan;4PHP Laboratory for Molecular Biology, Sendai, JapanWhen the a and b chains were separated from human oxyhemoglobin (HbO2), each individual chain was o xidizedeasily ... Shikama, PHP Laboratory for MolecularBiology, Nakayama-Yoshinari 1-16-8, Sendai 989±3203, Japan.Fax: + 81 22 278 6180, E-mail: shikama@mail.cc.tohoku.ac.jpAbbreviations: p-MB, sodium p-hydroxymercuribenzoate.(Received ... chains [8]. In the autoxidation reaction of HbO2,aswellasMbO2,pH can affect the rate in many d ifferent ways. Recent kineticand thermodynamic studies of the stability of mammalianoxymyoglobins...
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Báo cáo Y học: Substrate specificity of human kallikrein 2 (hK2) as determined by phage display technology docx
... pentamer library. DNA sequence analysis reveal thatan arginine appears in 40 clones at the P1 site and only onepeptide is cleaved at a lysine. Among the substrateshydrolysed at an arginine, ... [8]. In addition, in vitro studies have shown thathK2 can activate urokinase-type plasminogen activator [9]and inactivate plasminogen activator inhibitor-1 [10] leadingto the activation of urokinase ... selectiveinhibitors for cancer chemotherapy as well as acceleratethe discovery of new targets. Phage display has alreadyopened new avenues in kallikrein research that may furtherreinforce the...
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Báo cáo y học: "Chlamydia trachomatis Infection of Human Trophoblast Alters Estrogen and Progesterone Biosynthesis: an insight into role of infection in pregnancy sequelae." doc
... tested for mycoplasma contamination periodically by staining with 4,6 diamine-2-phenyl indole dihydrochloride (Boehringer, Mannheim, Germany). Chlamydia trachomatis culture Chlamydia trachomatis ... following chlamydia infection. The possibility of replicating these findings is the basis of an on-going in vivo study in our laboratory. Chlamydia trachomatis uniquely harbors eukaryotic ... (Kodak, Chicago, IL) Protein Estimation Protein assay on each sample was done by the bicinchoninic acid-copper (II) sulphate reagent assay system [21]. Statistical Analysis All data are expressed...
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Báo cáo y học: "Comparison between single antiplatelet therapy and combination of antiplatelet and anticoagulation therapy for secondary prevention in ischemic stroke patients with antiphospholipid syndrome"
... any difference in effectiveness for secondary preven-tion between single antiplatelet (aspirin) and single anticoagulant (warfarin) therapy. Our results indicate that combination therapy may ... (63 years) was significantly older than that in typical APS studies (34 years). Third, the target INR in WAPS [8] was for cardiogenic stroke caused by non-valvular Af. The dosage of aspirin in ... think sin-gle antiplatelet therapy may be less effective for the secondary prevention of stroke than the combination of antiplatelet and anticoagulant therapy. We have examined endothelial...
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Báo cáo y học: "Thioglycosides as inhibitors of hSGLT1 and hSGLT2: Potential therapeutic agents for the control of hyperglycemia in diabetes"
... the inhibitory effect of some thioglycosides synthesized in our laboratory on human hSGLT1 and hSGLT2 –as a potential therapeutic alternative for the control of hyperglycemia, particularly for ... Dort-mund, Germany; 2. Max Planck Institute for Chemical Ecology, Dortmund, Germany Correspondence to: Francisco Castaneda, MD, Laboratory for Molecular Pathobiochemistry and Clinical Research, ... thioglycosides are im-portant to consider because they are not hydrolysed by β-glucosidases in the intestine and can be adminis-tered orally [27]. Therefore, the aim of the present study was to evaluate...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... GGC AGA GAA TAC GTT TAT ACT AA 3¢C14L 5¢ TCT CAG ATT GAC TAC TAC AAC TT 3¢C15U 5¢ CAT GAA AAG CAT TTC AAA AA 3¢C15L 5¢ TAA AAA ACA GTG GTT ACC AA 3¢C16U 5¢ CTA AAG AGT ACA CTA TCC TCA C 3¢C16L ... amplificationbetweenC7UandC8L.Primersinitalicswereusedinthegenotyping of allele M.C1U5¢ GAG AGG AAC TGA ACA GAA CAT TG 3¢C1L 5¢ CAA CTG CGT ATT AGT GAA GAA TG 3¢C2U 5¢ AAT CAA ATT TTA TTA TAA GAC C 3¢C2L5¢ AAT AGC TAA TTA GAG ... TTC CAT GGC TTG ATC 3¢C19L 5¢ ATA CAC ACA AAC TCA CAA GG 3¢MWU 5¢ CAA CAT ATT TTA AAT AAA ATT GAC AAT 3¢C9LM* 5¢ ATA AAA ATG GTA TAC CTC ACT TGT*C 3¢C9UM1 5¢ TAA CAA TGA TTC TCT TTC TTT TAG...
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Báo cáo Y học: Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase b subunit in the energy coupling mechanism of the Na+ pump ppt
... of Y2 29, which subsequently can be replaced by a Na+-ion again, initiating the decarboxylation of the newlybound carboxybiotin. This interpretation can explain why in the S38 2A mutant decarboxylation ... recently that the initial velocity of oxaloacetate decarboxylation has sigmoidal dependence onFig. 2. SDS/PAGE analysis of a selection of mutant oxaloacetatedecarboxylases synthesized in E. coli and ... accumu-lated upon incubation with [4-14C]oxaloacetate (not shown).The mutant enzyme therefore contained an intact carboxyl-transferase and an impaired carboxybiotin decarboxylaseactivity.Kinetic...
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