Báo cáo khoa học: "Neoangiogenesis in early cervical cancer: Correlation between color Doppler findings and risk factors A prospective observational study" pot
Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... establishing the kinetic constants at optimal and physiological pH values, analyzing the effect of activators and inhibitors, and investigating the storage stability and oligo- meric state. Determination ... recombinant glycolytic enzymes and flux control analysis Emma Saavedra, Rusely Encalada, Erika Pineda, Ricardo Jasso-Cha ´ vez and Rafael Moreno-Sa ´ nchez Departamento de Bioq...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
... amplified from plasmid pHook (Invitrogen) using primers Z1 (5-CCGATATCGTGTTGACAATT AATC-3¢)andZ2(5¢-CCGATATCCAGACATGATAA GATAC-3¢). All primers contain an EcoRV restriction site and resulting PCR products, ... Then, plasmid pBSK+D2 was amplified by PCR using Pfu turbo polymerase (Stratagene) and primers BU (5¢-CCGATATCATACTCTTCC-3¢)andBL(5¢-CC GATATCAGACCAAGTTTAC-3¢).Inthesameway,the zeo ge...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo khoa học: "Differences in the serum immunoglobulin concentrations between dairy and beef calves from birth to 14 days of age" pdf
... systemic invasion by microorganisms and septic disease during the neonatal period. Unabsorbed Igs and Igs re-secreted back into the gut play an important role in protection against intestinal disease ... without anticoagulant before suckling or colostrum-feeding (0 hrs), at one and four days, and thereafter at two-day intervals up to 14 days after birth. Blood samples were allowed t...
Ngày tải lên: 07/08/2014, 17:22
báo cáo hóa học:" Shiftwork in the Norwegian petroleum industry: overcoming difficulties with family and social life – a cross sectional study" pdf
... coping strategies scales were entered as independent variables. In general, the results of this analysis allowed us to use the dependent variables in sep- arate analyses, as shown in Table 2 and ... time to be with friends and to main- tain adequate social relations. Further, wishful thinking as a coping strategy was associated with problems in social and domestic/family life....
Ngày tải lên: 20/06/2014, 00:20
Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... (5¢-TTT TTC TAG AGC AGG GTA GGT TGA TTT CAT GTC GAA TG-3¢; additional XbaI site underlined) and OB 3 (5¢-AAA AGA ATT CTT AGA AGT CCC AGT CAT CGT C-3¢; additional EcoRI site underlined). The amplified ... nrdF + gene was sequenced by a primer walking approach. For DNA analysis, dnastar software (DNAS- TAR Inc., Madison, WI, USA) and clone manager 5.0 (Scientific & Educational Software,...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc
... IL, USA) in 1 m NaOH as standard (final concentration of BSA stock solution in 1 m NaOH was 1.8 mgÆmL )1 ). Fermentative capacity and steady-state fluxes The fermentative capacity is measured as the ... the capacities of hexokinase (HXK), aldolase (ALD), PGK, GPM, PYK and pyruvate decarboxylase (PDC) were upregulated. The capacity of alcohol dehy- drogenase (ADH) was downregulated and...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma pptx
... variants with improved membrane binding characteristics and hyper- anticoagulant activity. Binding ability was established using a SPR membrane binding assay and anticoagu- lant activity was assessed ... numbered lanes) was applied to each lane and visualized by silver staining. Protein C vari- ants and molecular weight markers (MWM) ran in each lane are indicated. The location of...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Changes in purine specificity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx
... cyaB1 and rPDE2 tandem GAF domains that dis- criminate between the adenine and guanine of cAMP and cGMP as primary ligands. Previous experiments investigating cGMP binding to GAF domains of mammalian ... domains in PDEs 2, 5, 6 and 11, and cAMP is the ligand for PDE10. Structurally related tandem GAF domains signalling via cAMP are present in the cyanobacterial adenylate cycla...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... antiparallel b-sheet (strands bC–bG) facing the interior of the phage particle, with an N-terminal hairpin (strands bAandbB) and two a- helices (aAandaB) shielding most of the upper surface of the b-sheet ... conformational chan- ges are analyzed by fluorescence spectra, light scattering, CD, HPLC, infectivity assays and the bis-8-anilinonaph- thalene-1-sulfonate (bis-ANS) binding assay....
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3-kinase pathways including thr ombin, dexamethasone, angiotensin II and prostaglandins ... nucleation and stabilizing actin filaments and swinholide A, a drug that sequesters G-actin as dimers [28]. As shown in Fig. 6A, cells treated with jasplakinolide assumed a diamond...
Ngày tải lên: 19/02/2014, 16:20