Báo cáo khoa học: "Alternative reconstruction after pancreaticoduodenectomy" pps

... uncut Roux reconstruction after gastrectomy: limitations of the stapling technique. Am J Surg 1995, 170:262-264. 6. Klaus A, Hinder RA, Nguyen JH, Nelson KL: Small bowel transit and gastric emptying after ... [5-7]. Conclusion The uncut Roux-en-Y offers significant advantages over the classic Whipple reconstruction and pylorus-preserving reconstruction. Our technique has eliminated d...

Ngày tải lên: 09/08/2014, 07:21

Ngày tải lên: 06/03/2014, 09:22

Ngày tải lên: 06/03/2014, 09:22

... iso- lated at a position that corresponds to the protein plus RNA between 50 and 100 nucleotides. After proteinase K digestion, the RNA is recovered from the membrane and ligated on its 3¢ end to

Ngày tải lên: 06/03/2014, 09:22

... mole- cules that inhibit splicing by specifically targeting indi- vidual SR proteins was developed [46]. After screening a collection of chemical compounds, one indole deriva- tive (IDC16) was discovered

Ngày tải lên: 06/03/2014, 09:22

... solution 6 [25]. Precipitate wasallowedtoformat37°C for 40 min and added to the cells (on 5 cm plates). After 4 –5 h i ncubation at 37 °Ca glycerol shock was performed. The medium was removed and 1.5 ... (v/v) glycerol was added for 90 s on SK-N-SH cells and 60 s on SH-SY5Y cells. Glycerol was removed after dilution with 2 m L NaCl/P i and the plates were washed three times with 3 mL NaCl...

Ngày tải lên: 16/03/2014, 18:20

... leaves a small extension of four amino acid residues at the N-terminus of each recombinant protein after removal of the His tag (Fig. S4). The kinetic differences between the normal and alternative ... Synechocystis as substrate. H-proteins carrying an N-terminal extension of four amino acids, which remain after thrombin cleav- age of the His tag, are labelled ‘+4’. Values are means ± SE...

Ngày tải lên: 23/03/2014, 04:20

... fluorescence energy resonance transfer; gp120, glycoprotein 120; SPA, staphylococcal protein A; SPPS, solid-phase peptide synthesis; T m , melting temperature. 2668 FEBS Journal 275 (2008) 2668–2676 ... different principles for their attachment to the surface. Here, either monomeric affibody proteins, produced by SPPS and containing site-specifically introduced biotin or cysteine groups for dire...

Ngày tải lên: 23/03/2014, 07:20

... Author Journal compilation ª 2008 FEBS to be effective in reversing the digoxin-induced toxicity after administering just a moderate stoichiometric excess [11], thus demonstrating the acute protective effect ... & Kayser H (1987) Molecular structure of the bilin binding protein (BBP) from Pieris brassicae after refinement at 2.0 A ˚ resolu- tion. J Mol Biol 198, 499–513. 25 Hauptman PJ...

Ngày tải lên: 23/03/2014, 07:20

... that resides midway between the Gln and UTP binding sites (Fig. 1). Our observations suggest that, after binding to L109A CTPS, p assage of exogenous NH 3 or its analogues through the NH 3 tunnel ... pH 8.0) containing EGTA (0.5 m M ) using a thrombin ratio of 0.5 unitsÆmg )1 of target protein. After 8 h at 25 °C, cleavage was complete and the biotinylated thrombin was removed from the re...

Ngày tải lên: 23/03/2014, 13:20