... aheme covalently attached to the polypeptide chain via twothioether bonds, formed from the vinyl groups of the hemeand the two cysteine residues in the consensus Cys-X-X-Cys-His motif [1–4]. ... proteins in the native state are stabilized by 10–20 kJÆmol)1compared with those in the denatured state; the energy is equivalent to the formation of only a few hydrogenbonds. Therefore, to ... T., Yamazaki, K., Kyogoku, Y. &Kobayashi, Y. (1998) Solution structure of thermostable cyto-chrome c-552 from Hydrogenobacter thermophilus determined by1H-NMR spectroscopy. Biochemistry...