Báo cáo y học: "Shared expression of phenotypic markers in systemic sclerosis indicates a convergence of pericytes and fibroblasts to a myofibroblast lineage in fibrosis" pdf

... functionally distinct with regards to production of cytokines and extracellular matrix [16,17] and it was recently demonstrated that only Thy-1 +ve fibroblasts are capable of differentiating into myofibroblasts after ... of myofibroblasts in dcSSc skinDetection of myofibroblasts in dcSSc skin. Cryosections from (a) nor- mal and (b-f) dcSSc skin were stained with an antib...

Ngày tải lên: 09/08/2014, 07:20

... 5'-GCAGACAGTGAAACACCAACTATAC-3'; ∆BLyS sense 5'-CAGAAGAAACAGGATCTTACACAT-3'; and full- length BLyS/∆BLyS anti-sense 5'-TGCCAGCTGAATAG- CAGGAATTAT-3'. A 165 bp amplicon ... in normal individuals, and RA, and SLE patients. (a) Plasma from normal individuals (Nl), and RA and SLE patients were assayed for BLyS levels by ELISA. Each symbol indicates a...

Ngày tải lên: 09/08/2014, 07:20

... gene expression profiling of human erythrocyte could be an important key for under- standing the machinery of anucleate protein synthesis and its meaning in the pathophysiology of diseases. Acknowledgements ... in human RBCs encoding initiation, activation and regulation of transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD finger- DNA-bindi...

Ngày tải lên: 03/11/2012, 10:58

... promoter TGA AGC TGG AAA CCA TCA TTC AAA ACA TTA GCA GGA ATT TTC 52 343 Lower promoter GGA GTT CTG CCA GGG AAC CAC GAC AGG GGA GAA CGC CAC TTA 57 587 Exon 1 GTG CTG CCT GAG AAG GAT TG GAA AGT GCC ... were incubated with a specific polyclonal antiserum against recombinant cystatin F and subsequently hybridized with a secondary FITC labelled antibody against rabbit IgG. A vesicular stain...

Ngày tải lên: 21/02/2014, 01:21

... a- D -galactopyranoside, a- L -arabinopyranoside, b- L -arabinopyranoside, a- L -arabino-furanoside, a- D -man- nopyranoside, a- D -mannopyranoside, a- D -fucopyranoside, a- D -xylopyranoside, a- L -rhamnopyranoside) ... The crystal structures of Sinapis alba myro- sinase and a covalent glycosyl±enzyme intermediate provide insights into the substrate recogn ition and a...

Ngày tải lên: 08/03/2014, 16:20

... Hidekazu Saito 2 , Hirohisa Kurachi 2 and Junichi Fujii 1 Departments of 1 Biochemistry, 2 Obstetrics and Gynecology, and 3 Urology, Yamagata University School of Medicine, Yamagata, Japan; 4 Cell ... function of GR in the male genital tract of the rat was examined by meas- uring its enzymatic activity and examining the gene expression and localization of the protein....

Ngày tải lên: 17/03/2014, 17:20

... IgA, pIgA and SIgA. SDS/PAGE and Western blot of untransfected CHO-K1 cells (lane 1), IgA-29 (lane 2), pIgA-D (lane 3 and 5) and SIgA-3 (lane 4 and 6) detected by antihuman IgA or strippedandredetectedwithantihumanSC,as indicated. ... of individual transfectants Lars Norderhaug 1 , Finn-Eirik Johansen 2 and Inger Sandlie 3 1 Antibody Design AS, Nesoddtangen, Norway; 2 Departme...

Ngày tải lên: 18/03/2014, 01:20

... which are components of the eukaryotic cytoskeleton. Misfolded actin and tubulin appear to bind to the apical domain of the CCT ring [9,10] in a nearly native conformation [10,11]. Substrate binding ... [8] whereas GroEL has a sevenfold symmetry and is made of a single polypeptide chain. In addition, and in contrast to GroEL, CCT binds and folds a small range...

Ngày tải lên: 23/03/2014, 21:20

... Subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus roseus and Tabernamontana divaricata. Plant ... neuroleptic reserpine, the antihypertensive ajmalicine and the anti- arrhythmic ajmaline. The complex chemical structure of ajmaline, an alkaloid from the Indian medicinal plant Rau...

Ngày tải lên: 24/03/2014, 03:21

... recombinant NaoA after DEAE-Sepharose Fast Flow chromatography; lane 6, standard molecular mass markers (phosphorylase b,97kDa;BSA,66kDa; ovalbumin, 45 kDa). Fig. 3. Assay of NaoA activity. (A) ... of NaoA was determined from a standard curve of pI and migration distance (cm) of protein standards. Enzyme assay and analytical methods E. coli BL21(DE3) containing plasmid pNA101...

Ngày tải lên: 31/03/2014, 08:20