Báo cáo y học: "Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis" pot

... con- sidered statistically significant. Results Gene expression profile in joints at onset of arthritis To define the gene expression profile at early stages of CIA, we used the murine Affymetrix oligonucleotide ... http://arthritis-research.com/content/7/4/R868 Page 868 of 876 (page number not for citation purposes) Vol 7 No 4 Research article Gene expression profile...

Ngày tải lên: 09/08/2014, 06:23

... infliximab/methotrexate combination in methotrexate-resist- ant patients with very active disease. Materials and methods Patients A total of 33 patients, fulfilling the American College of Rheu- matology (ACR) ... to infliximab. Thirty three patients with very active disease (Disease Activity Score 28 >5.1) that resisted weekly methotrexate therapy were given infliximab at baseline...

Ngày tải lên: 09/08/2014, 08:22

... information greatly facilitated the discovery of novel disease-related genes and the mapping of signature genes for early diagnosis. More specifically, polynucleotide or oligonucleotide arrays have been ... 2000, 233:131-140. 4. Watanabe N, Ando K, Yoshida S, Inuzuka S, Kobayashi M, Matsui N, Okamoto T: Gene expression profile analysis of rheumatoid synovial fibroblast culture...

Ngày tải lên: 09/08/2014, 06:22

... by its ability to down- regulate RANTES expression and by stimulating the synthesis of MMP-1 and IL-1 [50]. We hypothesize that the relatively stable expression and tight regulation control of ... carbohydrate TLR ligands fall into the category of oli- gosaccharides generated by endoglycosidases, enzymes that Figure 7 Enzyme activities of synovial fibroblast samples of r...

Ngày tải lên: 09/08/2014, 14:20

... 10 min incubation at 20°C) at 42°C for 50 min by using 500 ng of total RNA in 5.5 µl of diethyl pyrocarbon- ate water (0.1% diethyl pyrocarbonate-treated water), 2 µl of 5 × first-strand buffer ... oligonucleotides of c-myc can induce apoptosis and downregulation of Fas expression in synoviocytes [37]. The present study demonstrates the potency of IL-17, IL-1β, and TNF-α...

Ngày tải lên: 09/08/2014, 01:22

... suggest that many of the genes in the autoimmune signature display high levels of heritability (K Maas and T Aune, unpublished data). Symbolic discriminant analysis of microarray data Relationships ... signatures The autoimmune signature is only one of many gene expression patterns that will be of importance in patients with diseases that involve the immune system (Fig. 4). It i...

Ngày tải lên: 09/08/2014, 01:23

... Therapy Vol 7 No 1 Rioja et al. R116 preparation. CC and SG performed the microarray experi- ments and statistical analysis of the array data. MD and PL carried out the study design and collaborated ... CL: Discovery of distinctive gene expression profiles in rheumatoid synovium using cDNA microarray technology: evidence for the existence of multiple pathways of tissue destr...

Ngày tải lên: 09/08/2014, 06:22

... resolution of inflammation. We hypothesized that this involves tissue-specific regulation of glucocorticoids, mediated via differential regulation of the enzyme 11β-hydroxysteroid dehydrogenase type ... using primary cul- tures of fibroblasts isolated from matched synovium, bone marrow and skin. Analysis of mRNA from these cells indicated that expression of 11β-HSD1 was greate...

Ngày tải lên: 09/08/2014, 08:22

... IL-1β, the association of TNF-α and IL-17, and the association of TNF-α and IL-1β. Materials and methods Patients and synovial biopsies Twenty-five patients (18 women and 7 men, median age 55.2 years, ... specific pathways such as T-cell-mediated immunity, macrophage-mediated immunity, cytokine- and chemokine-mediated signaling pathways, major histocompatibility complex II...

Ngày tải lên: 09/08/2014, 14:20

... apoptosis may be related to the mitochon- drial pathway BCL-2 family ge nes and the TNFSF10 gene of the death re ceptor signaling pathway. Moreover, the SPP1andCREBBP genes in the TGF-b pathway may also ... intrin- sicmitochondrialpathwayaswellastheTNFS F10 gene in the death receptor signaling pathway (Figure 2) [20]. Upregulation of the TNFSF10 gene activated the death rec eptor signal...

Ngày tải lên: 10/08/2014, 21:23