Báo cáo y học: "Regulation of CD154-induced interleukin-12 production in synovial fluid macrophage" pps

... using the Prime-a-gene labeling system (Promega). Double-positive clones were purified by secondary and tertiary r ounds of screening and genomic DNA inserts were purified using poly(ethylene glycol) ... promoter is independent of TATA-binding-protein-associated factors and initiator-binding proteins. Mol. Cell Biol. 17, 2973–2984. 32. Weis, L. & Reinberg, D. (1992) Transcription by RNA...

Ngày tải lên: 08/03/2014, 16:20

... translo- cation and in poly(U)-directed polyphenylalanine synthesis [26,27], by preventing it from binding to the ribosome [28]. Early data showed that the phosphorylation of eEF2 was increased by very low ... members of main protein kinase superfamily, Ryazanov coined the term Ôa-kinaseÕ for this unusual group of enzymes. Further discussion of this family may be found in a recent...

Ngày tải lên: 23/03/2014, 21:20

... and restrain the inner NP region. Molecular changes in degenera- tion include altered matrix synthesis, including a decrease in glycosaminoglycan production and an increase in collagen type I within ... S, Amin R, Kunisch E, Jasin H, Kinne R: Preferential induction of prodestructive matrix metalloproteinase-1 and proinflammatory interleukin 6 and prostaglandin E2 in rheu- matoid a...

Ngày tải lên: 09/08/2014, 14:20

... with fibromyalgia syndrome. Pain 2003, 102:87-95. 44. Martinez-Lavin M: Fibromyalgia as a sympathetically main- tained pain syndrome. Curr Pain Headache Rep 2004, 8:385-389. 45. Grimm DR, Cunningham ... The protocol con- sisted of 7 phases (Figure 1): 1. Adaptation (30 minutes): sitting quietly in a chair with eyes open. 2. Resting BL (4 minutes): sitting quietly with eyes open and to mov...

Ngày tải lên: 09/08/2014, 07:20

... surgery is not required [1]. • 4/3 joint training pathway requires 4 years of gen- eral surgery residency training followed by 2 years of cardiothoracic surgery fellowship, both part of the training ... laboratory. In Ger- many, research training takes place simultaneously with clinical training. This is facil itated by flexibility of training in Germany. 6. Change from a trainee...

Ngày tải lên: 10/08/2014, 09:23

... fluid levels of aggrecan turnover markers in inflamed jointsSynovial fluid levels of aggrecan turnover markers in inflamed joints. Synovial fluid concentrations of (a) glycosaminoglycans (GAG) ... and a transient inflammatory stimulus may limit Figure 2 Synovial fluid inflammatory mediators and matrix metalloproteinase activity in inflamed jointsSynovial fluid inflam...

Ngày tải lên: 09/08/2014, 13:22

... bind are similar and binding of these factors is independently regulated by different coactivators in a cell-cycle-dependent manner. The distinct functions of Sp1 and Sp3 in the regulation of ... presence of a potent inhibitory domain in Sp3 [34]. The relative abundance of Sp1 and Sp3 allows the fine tuning of the regulation of gene activities. In endothelial cells that c...

Ngày tải lên: 22/02/2014, 07:20

... these proteins can be activated independently, suggesting that each link is func- tional [5–7]. In addition, in megakaryocytic cell lines and in primary megakaryocytes, the precursor of platelets, ... protein con taining the RAS-b inding domain o f RAF. Precipi- tated proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig (top). Proteins of wh...

Ngày tải lên: 08/03/2014, 16:20

... phosphorylation of conserved tyrosine and threonine residues within the catalytic domain. This phosphorylation is mediated by dual specificity protein kinases, members of the MAPK kinase (MKK) family. ... systems. REGULATION OF SAPK SIGNAL PATHWAYS BY PROTEIN PHOSPHATASES IN YEAST CELLS A molecular g enetic analysis of ye ast cells indicated that two distinct protein phosphatase grou...

Ngày tải lên: 17/03/2014, 23:20

... the increase of intracel- lular cyclic AMP and calcium levels. Effect of intracellular cAMP level increase The involvement of the cAMP pathway was evaluated using three approaches, all inducing ... by a specific extracellular domain displaying low sequence homology, and highly conserved transmembrane and cytoplasmic domains. How- ever, the cytoplasmic domain of all four syndecans conta...

Ngày tải lên: 17/03/2014, 23:20